Abstract
Ten plant species were grown in constructed wetlands (CWs) to remediate water containing 2% (w/v) crude oil. The plant species with better growth and biomass production were Typha latifolia and Cyperus laevigatus, and they were significantly correlated (R2 = 0.91) with hydrocarbon degradation. From T. latifolia and C. laevigatus, 33 hydrocarbon-degrading bacterial strains were isolated from the rhizosphere, and root and shoot interiors. More diversified bacteria were found in the rhizosphere and endosphere of C. laevigatus than those of T. latifolia. The predominant cultural hydrocarbon-degrading bacteria were shown to belong to the genera Pseudomonas, Acinetobacter and Bacillus. In addition to genes involved in hydrocarbon degradation, most of the bacteria displayed multiple plant growth promoting (PGP) activities. This study suggests the importance of selecting suitable bacterial strains with hydrocarbon degradation and PGP activities for improving the efficacy of CWs used in remediating water contaminated with crude oil.
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References
Afzal M, Yousaf S, Reichenauer TG, Kuffner M, Sessitsch A (2011) Soil type affects plant colonization, activity and catabolic gene expression of inoculated bacterial strains during phytoremediation of diesel. J Hazard Mater 186:1568–1575
Bhattacharyya PN, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World J Microbiol Biotechnol 28:1327–1350
Brix H (1997) Do macrophytes play a role in constructed treatment wetlands? Water Sci Technol 35:11–17
Das N, Chandran P (2010) Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnol Res Int 2011:941810. https://doi.org/10.4061/2011/941810
Fatima K, Afzal M, Imran A, Khan QM (2015) Bacterial rhizosphere and endosphere populations associated with grasses and trees to be used for phytoremediation of crude oil contaminated soil. Bull Environ Contam Toxicol 94:314–320
Glick BR (2010) Using soil bacteria to facilitate phytoremediation. Biotechnol Adv 28:367–374
Khan S, Afzal M, Iqbal S, Khan QM (2013) Plant-bacteria partnerships for the remediation of hydrocarbon contaminated soils. Chemosphere 90:1317–1332
Kirk JL, Klirnomos JN, Lee H, Trevors JT (2002) Phytotoxicity assay to assess plant species for phytoremediation of petroleum-contaminated soil. Bioremediat J 6:57–63
Li R-L, Liu B-B, Zhu Y-X, Zhang Y (2016) Effects of flooding and aging on phytoremediation of typical polycyclic aromatic hydrocarbons in mangrove sediments by Kandelia obovata seedlings. Ecotoxicol Environ Saf 128:118–125
Liu X, Wang Z, Zhang X, Wang J, Xu G, Cao Z, Zhong C, Su P (2011) Degradation of diesel-originated pollutants in wetlands by Scirpus triqueter and microorganisms. Ecotoxicol Environ Saf 74:1967–1972
Mahmood T, Zhang J, Zhang G (2016) Assessment of constructed wetland in nutrient reduction, in the commercial scale experiment ponds of freshwater prawn Macrobrachium rosenbergii. Bull Environ Contam Toxicol 96:361–368
Reva ON, Hallin PF, Willenbrock H, Sicheritz-Ponten T, Tummler B, Ussery DW (2008) Global features of the Alcanivorax borkumensis SK2 genome. Environ Microbiol 10:614–625
Ruan X, Xue Y, Wu J, Ni L, Sun M, Zhang X (2006) Treatment of polluted river water using pilot-scale constructed wetlands. Bull Environ Contam Toxicol 76:90–97
Schneiker S, dos Santos VAPM, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C (2006) Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nat Biotechnol 24:997–1004
Shehzadi M, Afzal M, Khan MU, Islam E, Mobin A, Anwar S, Khan QM (2014) Enhanced degradation of textile effluent in constructed wetland system using Typha domingensis and textile effluent-degrading endophytic bacteria. Water Res 58:152–159
Siciliano SD, Fortin N, Mihoc A, Wisse G, Labelle S, Beaumier D, Ouellette D, Roy R, Whyte LG, Banks MK (2001) Selection of specific endophytic bacterial genotypes by plants in response to soil contamination. Appl Environ Microbiol 67:2469–2475
Singh T, Singh DK (2018) Assessing the bacterial community structure in the rhizoplane of wetland plants. Bull Environ Contam Toxicol 101:521–526
Sung K, Kim KS, Park S (2013) Enhancing degradation of total petroleum hydrocarbons and uptake of heavy metals in a wetland microcosm planted with Phragmites communis by humic acids addition. Int J Phytorem 15:536–549
Tara N, Afzal M, Ansari TM, Tahseen R, Iqbal S, Khan QM (2014) Combined use of alkane-degrading and plant growth-promoting bacteria enhanced phytoremediation of diesel contaminated soil. Int J Phytorem 16:1268–1277
Van Beilen JB, Funhoff EG (2007) Alkane hydroxylases involved in microbial alkane degradation. Appl Microbiol Biotechnol 74:13–21
Wang L, Wang W, Lai Q, Shao Z (2010) Gene diversity of CYP153A and AlkB alkane hydroxylases in oil-degrading bacteria isolated from the Atlantic Ocean. Environ Microbiol 12:1230–1242
Weyens N, van der Lelie D, Taghavi S, Vangronsveld J (2009) Phytoremediation: plant-endophyte partnerships take the challenge. Curr Opin Biotechnol 20:248–254
Wu T, Hong B, Zhou S, Zhao J, Xia C, Liu H (2012) Residues of HCHs and DDTs in soils and sediments of preconstructing urban wetland. Bull Environ Contam Toxicol 89:563–567
Ying X, Dongmei G, Judong L, Zhenyu W (2011) Plant-microbe interactions to improve crude oil degradation. Energy Procedia 5:844–848
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This research was supported by the Higher Education Commission, Pakistan (Grant No. 20-3854/R&D/HEC/14).
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Hashmat, A.J., Afzal, M., Fatima, K. et al. Characterization of Hydrocarbon-Degrading Bacteria in Constructed Wetland Microcosms Used to Treat Crude Oil Polluted Water. Bull Environ Contam Toxicol 102, 358–364 (2019). https://doi.org/10.1007/s00128-018-2518-y
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DOI: https://doi.org/10.1007/s00128-018-2518-y