Skip to main content
SpringerLink
Log in

Genotoxic Effects Induced by Cd+2, Cr+6, Cu+2 in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae)

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Genotoxic effects of Cd+2, Cr+6, and Cu+2 on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr+6 and Cu+2, and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd+2 by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd+2 and Cr+6 and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ahmed MK, Parvin E, Arif M et al (2010) Measurements of genotoxic potential of cadmium in different tissues of fresh water climbing perch Anabas testudineus (Bloch), using the comet assay. Environ Toxicol Pharmacol 30:80–84

    Article  CAS  Google Scholar 

  • Ahmed MK, Kundu GK, Al-Mamun MH et al (2013) Chromium (VI) induced acute toxicity and genotoxicity in freshwater stinging catfish, Heteropneustes fossilis. Ecotoxicol Environ Saf 92:64–70

    Article  CAS  Google Scholar 

  • Arkhipchuk VV, Garanko NN (2005) Using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. Ecotoxicol Environ Saf 62:42–52

    Article  CAS  Google Scholar 

  • Avigliano E, Schenone NF, Volpedo AV et al (2015) Heavy metals and trace elements in muscle of silverside (Odontesthes bonariensis) and water from different environments (Argentina): aquatic pollution and consumption effect approach. Sci Total Environ 506–507:102–108

    Article  Google Scholar 

  • Bopp S, Abicht H, Knauer K (2008) Copper-induced oxidative stress in rainbow trout gill cells. Aquat Toxicol 86:197–204

    Article  CAS  Google Scholar 

  • Cambier S, Gonzalez P, Durrieu G, Bourdineaud JP (2010) Cadmium-induced genotoxicity in zebrafish at environmentally relevant doses. Ecotoxicol Environ Saf 73:312–319

    Article  CAS  Google Scholar 

  • Carriquiriborde P, Ronco A (2002) Sensitivity of the neotropical teleost Odonthestes bonariensis (pisces, Atherinidae) to chromium (VI), copper (II), and cadmium (II). Bull Environ Contam Toxicol 69:294–301

    Article  CAS  Google Scholar 

  • Carriquiriborde P, Ronco AE (2008) Distinctive accumulation patterns of Cd(II), Cu(II), and Cr(VI) in tissue of the South American teleost, pejerrey (Odontesthes bonariensis). Aquat Toxicol 86:313–322

    Article  CAS  Google Scholar 

  • Collins AR, Oscoz AA, Brunborg G et al (2008) The comet assay: topical issues. Mutagenesis 23:143–151

    Article  CAS  Google Scholar 

  • Deventer K (1996) Detection of genotoxic effects on cells of liver and gills of B. rerio by means of single cell gel electrophoresis. Bull Environ Contam Toxicol 56:911–918

    Article  CAS  Google Scholar 

  • Filipič M (2012) Mechanisms of cadmium induced genomic instability. Mutat Res Mol Mech Mutagen 733:69–77

    Article  Google Scholar 

  • Frenzilli G, Nigro M, Lyons BP (2009) The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutat Res 681:80–92

    Article  CAS  Google Scholar 

  • Handy RD (1992) The assessment of episodic metal pollution. I. Uses and limitations of tissue contaminant analysis in rainbow trout (Oncorhynchus mykiss) after short waterborne exposure to cadmium or copper. Arch Environ Con Tox 22(1):74–81

    Article  CAS  Google Scholar 

  • Jindal R, Verma S (2015) In vivo genotoxicity and cytotoxicity assessment of cadmium chloride in peripheral erythrocytes of Labeo rohita (Hamilton). Ecotoxicol Environ Saf 118:1–10

    Article  CAS  Google Scholar 

  • Lushchak VI (2011) Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 101:13–30

    Article  CAS  Google Scholar 

  • Merlo C, Abril A, Amé MV et al (2011) Integral assessment of pollution in the Suquía River (Córdoba, Argentina) as a contribution to lotic ecosystem restoration programs. Sci Total Environ 409:5034–5045

    Article  CAS  Google Scholar 

  • Nickens KP, Patierno SR, Ceryak S (2010) Chromium genotoxicity: a double-edged sword. Chem Biol Interact 188:276–288

    Article  CAS  Google Scholar 

  • Rigacci LN, Giorgi ADN, Vilches CS et al (2013) Effect of a reservoir in the water quality of the Reconquista River, Buenos Aires, Argentina. Environ Monit Assess 185:9161–9168

    Article  CAS  Google Scholar 

  • Russo C, Rocco L, Morescalchi MA, Stingo V (2004) Assessment of environmental stress by the micronucleus test and the Comet assay on the genome of teleost populations from two natural environments. Ecotoxicol Environ Saf 57:168–174

    Article  CAS  Google Scholar 

  • Sanchez-Galan S, Linde AR, Garcia-Vazquez E (1999) Brown trout and European minnow as target species for genotoxicity tests: differential sensitivity to heavy metals. Ecotoxicol Environ Saf 43:301–304

    Article  CAS  Google Scholar 

  • Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191

    Article  CAS  Google Scholar 

  • Somoza GM, Miranda LA, Berasain GE et al (2008) Historical aspects, current status and prospects of pejerrey aquaculture in South America. Aquac Res 39:784–793

    Article  Google Scholar 

  • Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ (2012) Heavy metal toxicity and the environment. EXS 101:133–164

    Google Scholar 

  • USEPA (1991) Methods for the determination of metals in environmental samples. U.S. Environmental Protection Agency. EPA/600/4-91/010, Washington

  • Velma V, Tchounwou PB (2010) Chromium-induced biochemical, genotoxic and histopathologic effects in liver and kidney of goldfish, Carassius auratus. Mutat Res 698:43–51

    Article  CAS  Google Scholar 

  • Villar C, Stripeikis J, Tudino M et al (1999) Trace metal concentrations in coastal marshes of the Lower Paraná River and the Río de la Plata Estuary. Hydrobiologia 397:187–195

    Article  CAS  Google Scholar 

  • Yadav KK, Trivedi SP (2009) Sublethal exposure of heavy metals induces micronuclei in fish, Channa punctata. Chemosphere 77:1495–1500

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was funded by CONICET and ANPCyT of Argentina. We acknowledge the Buenos Aires Province Ministry of Agriculture Affairs, particularly to the Chascomús Aquaculture Station, for providing the O. bonariensis larvae.

Author information

Authors and Affiliations

  1. Instituto de Microbiología y Zoología Agrícola (IMyZA), Instituto Nacional de Tecnología Agropecuaria (INTA) – CONICET, Las Cabañas y De los Reseros s/n, Hurlingham, Buenos Aires, Argentina

    J. Gasulla

  2. Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata – CONICET, Calle 47 y 115 s/n (1900), La Plata, Buenos Aires, Argentina

    P. Carriquiriborde & A. E. Ronco

  3. Instituto de Genética Veterinaria “Fernando Noel Dulout” (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata – CONICET, Calle 60 y 117 (1900), La Plata, Buenos Aires, Argentina

    S. J. Picco, F. N. Dulout & J. C. de Luca

Authors
  1. J. Gasulla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. J. Picco
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Carriquiriborde
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. N. Dulout
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. E. Ronco
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. C. de Luca
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Carriquiriborde.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gasulla, J., Picco, S.J., Carriquiriborde, P. et al. Genotoxic Effects Induced by Cd+2, Cr+6, Cu+2 in the Gill and Liver of Odontesthes bonariensis (Piscies, Atherinopsidae). Bull Environ Contam Toxicol 96, 591–595 (2016). https://doi.org/10.1007/s00128-016-1774-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-016-1774-y

Keywords

Search

Navigation