Abstract
A rapid, sensitive and reliable analytical method for thidiazuron residues in apple and soil was established. The residual levels of the pesticide in apple and soil were determined by high performance liquid chromatography (HPLC) with UV detector. Samples of apple and soil were extracted with acetonitrile–water solutions, and then cleaned up by Florisil or C18 cartridges. The results showed good linearity (r 2 = 1.000) over the concentration range of 0.01–5.0 mg/L. Limits of quantification (LOQ) of the method were 0.01 mg/kg for both soil and apple. Recovery from the apple and soil samples were 83.36%–84.08% and 85.27%–89.83%, respectively, and the corresponding relative standard deviations (RSDs) of the recovery data were 0.155%–0.524% and 0.475%–4.79% for the three fortified levels (0.01, 0.1, 0.5 mg/kg). The analyte in the samples were further confirmed by electrospray ionization tandem mass spectrometry (ESI–MS/MS). It was demonstrated that the proposed method was simple and efficient, and particularly suitable for detecting thidiazuron residues in apple and soil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cai DL, Chen JX, Chen LH, Lie SQ, Wang CG (2009) Residue detection and degradation of 50% thidiazuron WP in leaves and seeds of cotton and soil. Modern Agrochem 8(5):40–43
Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thiadiazron on cytokinin autonomy and metabolism of N6-(2-isopentyl) [8–14C] adenosine in callus tissue of Phaseolus lunatus L. Plant Physiol 73:796–802
Fiola JA, Hassan MA, Swartz HJ, Bors RH, McNicols R (1990) Effect of thidiazuran, light influence rates and kanamycin in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant Cell Tiss Org Cult 20:223–228
Guo YZ, Zhang YT, Song SR, Liu L, Liu FL (2005) A method for determining residues of thidiazuron and its isomers in cotton seeds and soil. Chin J Pestic 44(3):123–124
Guo YZ, Zhang YT, Liu L, Shao H, Li H, Song SR, Li NJ (2010) Residual determination method of thidiazuron, diuron and their metabolites in soil. Anhui Agric Sci 38(2):856–857
Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgare L., high frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186:384–389
Potter TL, Marti L, Belflower S, Truman CC (2000) Multiresidue analysis of cotton defoliant, herbicide, and insecticide residues in water by solid-phase extraction and GC-NPD, GC-MS, and HPLC diode array detection. J Agric Food Chem 48:410–418
Tekel J, Hatrfk S (1996) Pesticide residue analyses in plant material by chromatographic methods: clean-up procedures detectors. J Chromatogr A 754:397–410
Wang X, Liu XG, Dong FS, An JJ, Zhen YQ (2010) Residue and degradation of thidiazuron in melon and soil. Environ Chem 29(2):277–280
Zhuang WJ (2010) The global regulations on maximum residue limits (MRLs) for pesticides in foodstuffs and feedetuffs, vol 1. Chemical Industry Press, Beijing, p 456
Acknowledgments
This work was supported by grants from Jiangsu Huifeng Agrochemical Co., Ltd. (Jiangsu, China) and the Institute for the Control of Agrochemicals, Ministry of Agriculture of the People’s Republic of China.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hu, JY., Hu, YQ., Chen, Y. et al. High Performance Liquid Chromatography Method for Residues Analysis of Thidiazuron in Apple and Soil. Bull Environ Contam Toxicol 87, 448–451 (2011). https://doi.org/10.1007/s00128-011-0383-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-011-0383-z