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Comparative Antioxidant Profiling of Tolerant and Sensitive Varieties of Brassica juncea L. to Arsenate and Arsenite Exposure

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Abstract

Comparative antioxidant profiling of tolerant (TPM-1) and sensitive (TM-4) variety of Brassica juncea L. was performed after exposure to arsenate [As(V)] and arsenite [As(III)]. TPM-1 demonstrated higher accumulation of As upon exposure to both 500 μM As(V) and 250 μM As(III) (49 and 37 μg g−1 dw after 15 days) as compared with that observed in TM-4. The activities of various antioxidant enzymes and the level of glutathione and proline demonstrated, in general, a comparatively better response in TPM-1 than in TM-4 that presumably allowed TPM-1 to tolerate higher As concentrations as compared with that of TM-4.

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References

  • Ahsan N, Lee D-G, Alam I, Kim PJ, Lee JJ, Ahn Y-O, Kwak S-S, Lee I-J, Bahk JD, Kang KY, Renaut J, Komatsu S, Lee B-H (2008) Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics 8:3561–3576

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  Google Scholar 

  • De Tullio MC, Gara LD, Paciolla C, Arrigoni O (1998) Dehydroascorbate-reducing proteins in maize are induced by the ascorbate biosynthesis inhibitor lycorine. Plant Physiol Biochem 36:433–440

    Article  Google Scholar 

  • Drążkiewicz M, Skórzyńska-Polit E, Krupa Z (2003) Response of ascorbate–glutathione cycle to excess copper in Arabidopsis thaliana (L.). Plant Sci 164:195–202

    Article  Google Scholar 

  • Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plant 92:696–717

    Article  CAS  Google Scholar 

  • Gupta M, Sharma P, Sarin NB, Sinha AK (2009) Differential response of arsenic stress in two varieties of Brassica juncea L. Chemosphere 74:1201–1208

    Article  CAS  Google Scholar 

  • Hartley-Whitaker J, Ainsworth G, Meharg AA (2001) Copper- and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity. Plant Cell Environ 24:713–722

    Article  CAS  Google Scholar 

  • Hemeda HM, Klein BP (1990) Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. J Food Sci 55:184–185, 192

    Article  CAS  Google Scholar 

  • Hissin PJ, Hilf R (1976) A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74:214–226

    Article  CAS  Google Scholar 

  • Kaiser G, Kleiner O, Beisswenger C, Batschauer A (2009) Increased DNA repair in Arabidopsis plants overexpressing CPD photolyase. Planta 230:505–515

    Article  CAS  Google Scholar 

  • Khan I, Ahmad A, Iqbal M (2009) Modulation of antioxidant system for arsenic detoxification in Indian mustard. Ecotoxicol Environ Safety 72:626–634

    Article  CAS  Google Scholar 

  • Lee D, Schroeder J (2002) Phenotypic analysis of Arabidopsis mutants:heavy metal stress. In: Weigel D, Glazerbrook J (eds) Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, pp 75–77

    Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randal RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Mishra S, Srivastava S, Tripathi RD, Trivedi PK (2008) Thiol metabolism and antioxidant system complement each other during arsenate detoxification in Ceratophyllum demersum L. Aquat Toxicol 86:205–215

    Article  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione. Keeping active oxygen under control. Annu Rev Plant Physiol Mol Biol 49:249–279

    Article  CAS  Google Scholar 

  • Norton GJ, Lou-Hing DE, Meharg AA, Price AH (2008) Rice–arsenate interactions in hydroponics: whole genome transcriptional analysis. J Exp Bot 59:2267–2276

    Article  CAS  Google Scholar 

  • Sharma SS, Dietz K-J (2009) The relationship between metal toxicity and cellular redox balance. Trends Plant Sci 14:43–50

    Article  CAS  Google Scholar 

  • Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413

    Article  CAS  Google Scholar 

  • Srivastava S, Mishra S, Tripathi RD, Dwivedi S, Gupta DK (2006) Copper-induced oxidative stress and response of antioxidants and phytochelatins in Hydrilla verticillata (L.f.) Royle. Aquat Toxicol 80:405–415

    Article  CAS  Google Scholar 

  • Srivastava S, Mishra S, Tripathi RD, Dwivedi S, Trivedi PK, Tandon PK (2007) Phytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrilla verticillata (L.f.) Royle. Environ Sci Technol 41:2930–2936

    Article  CAS  Google Scholar 

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Acknowledgments

SS is thankful for the award of KSKRA fellowship from Department of Atomic Energy (DAE).

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Correspondence to Stanislaus F. D’Souza.

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Srivastava, S., Srivastava, A.K., Suprasanna, P. et al. Comparative Antioxidant Profiling of Tolerant and Sensitive Varieties of Brassica juncea L. to Arsenate and Arsenite Exposure. Bull Environ Contam Toxicol 84, 342–346 (2010). https://doi.org/10.1007/s00128-010-9937-8

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  • DOI: https://doi.org/10.1007/s00128-010-9937-8

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