Abstract
By-products of a two-phase and a three-phase olive oil mill process treated in an anaerobic fermentation system for hydrogen production, were evaluated by three bioassays: the zebrafish Danio rerio embryo test and two microbiotests, Thamnotoxkit F and Daphtoxkit FTM pulex. Samples from both processes were classified as “very toxic” with LC50 values ranging from 1.52% (T. platyurus 24 h test) to 4.48% (D. pulex 48 h-LC50). Toxicity values were differently correlated to physicochemical parameters showing different degree of influence. The treated effluents of both process systems remained very toxic showing the necessity for further treatment, aiming to environmentally safe discharges.
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Acknowledgments
We wish to thank Dr. Eleni Koutrouli and professor Gerasimos Lyberatos, University of Patras, Department of Chemical Engineering, Laboratory of Biochemical Engineering and Environmental Technology, Greece. This work could not have been completed without the collaboration with Dr. Koutrouli who conducted the effluent treatment in the anaerobic fermentation system.
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Rouvalis, A., Iliopoulou–Georgudaki, J. Comparative Assessment of Olive Oil Mill Effluents from Three-phase and Two-phase Systems, Treated for Hydrogen Production. Bull Environ Contam Toxicol 85, 432–436 (2010). https://doi.org/10.1007/s00128-010-0106-x
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DOI: https://doi.org/10.1007/s00128-010-0106-x