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Levels of DNA Adducts in the Blood and Follicular Fluid of Women Undergoing In Vitro Fertilization Treatment and Its Correlation with the Pregnancy Outcome

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Abstract

This study is designed to investigate the impact of DNA damage on pregnancy and fertilization rate outcome in a sub-sample of women undergoing IVF treatment. Blood and follicular fluid samples (n = 60) were analyzed for DNA adducts. While no BPDE-DNA adducts were detected, other unknown lipophilic adducts were seen in blood and follicular fluid. Women who failed to achieve pregnancy had higher DNA adducts in follicular fluid than those who succeeded (p < 0.05). Follicular fluid cotinine levels were associated with DNA adduct levels in blood and follicular fluid (p < 0.05). Evaluation of DNA damage resulting from oxidative stress could have a role in predicting IVF success rate.

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Acknowledgments

The authors are thankful to the staff of the IVF clinic and participants for their cooperation during the study. They are; also, indebted to King Faisal Specialist Hospital and Research Centre for its support (RAC#2010 006).

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Authors and Affiliations

  1. Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh, 11211, Saudi Arabia

    Iman Al-Saleh & Inaam El-Doush

  2. Departments of Biotechnology, Microbiology and Bioinformatics, Integral University, Kursi Road, Lucknow, 226026, India

    Jamal Arif

  3. Pathology Laboratory Medicine Department, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh, 11211, Saudi Arabia

    Serdar Coskun

  4. Obstetrics and Gynecology Department, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh, 11211, Saudi Arabia

    Kamal Jaroudi & Abdulaziz Al-Shahrani

  5. Disease Control Strategy Group, Liverpool School of Tropical Medicine, Liverpool, Pembroke Place, L3 5QA, UK

    Gamal El-Din Mohamed

Authors
  1. Iman Al-Saleh
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  2. Inaam El-Doush
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  3. Jamal Arif
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  4. Serdar Coskun
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  5. Kamal Jaroudi
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  6. Abdulaziz Al-Shahrani
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  7. Gamal El-Din Mohamed
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Corresponding author

Correspondence to Iman Al-Saleh.

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Al-Saleh, I., El-Doush, I., Arif, J. et al. Levels of DNA Adducts in the Blood and Follicular Fluid of Women Undergoing In Vitro Fertilization Treatment and Its Correlation with the Pregnancy Outcome. Bull Environ Contam Toxicol 84, 23–28 (2010). https://doi.org/10.1007/s00128-009-9889-z

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  • DOI: https://doi.org/10.1007/s00128-009-9889-z

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