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Use of Behavioral Responses of Rainbow Trout Oncorhynchus mykiss in Identifying Sublethal Exposure to Hexavalent Chromium

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Abstract

Laboratory tests were conducted in a flow-through apparatus on 1-year-old rainbow trout Oncorhynchus mykiss to evaluate the sensitivity of a number of their behavioral responses to hexavalent chromium (Cr6+). Test fish were exposed to Cr6+ concentrations corresponding to 0.001–1 parts of the rainbow trout 96-h LC50 (0.029–28.5 mg Cr/L, respectively) in short-term (15 min) tests. Sensitivity parameter responses could be arranged into the following sequence: latent period of detection response = locomotor activity > gill ventilation frequency > coughing rate. All the rainbow trout responses were sensitive behavioral indicators of sublethal exposure. Behavioral responses meet the criteria as rapid tools for bioassay testing and could be easily standardized using Cr6+ as a reference toxicant.

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References

  • Anestis I, Neufeld J (1986) Avoidance-preference reactions of rainbow trout (Salmo gairdneri) after prolonged exposure to chromium (VI). Wat Res 20:1233–1241. doi:10.1016/0043-1354(86)90152-1

    Article  CAS  Google Scholar 

  • ASTM (2008a) Standard guide for measurement of behavior during fish toxicity tests. E1711–95(2008). Am Soc Test Mater, 12 pp. doi:10.1520/E1711-95R08

  • ASTM (2008b) Standard guide for ventilatory behavioral toxicology testing of freshwater fish. E1768–95(2008). Am Soc Test Mater, 10 pp. doi:10.1520/E1768-95R08

  • Atchison GJ, Henry MG, Sandheinrich MB (1987) Effects of metals on fish behavior: a review. Environ Biol Fish 18:11–25. doi:10.1007/BF00002324

    Article  Google Scholar 

  • Brown SB, Evans RE, Thompson BE, Hara TJ (1982) Chemoreception and aquatic pollutants. In: Hara TJ (ed) Chemoreception in fishes. Dev Aquac Fish Sci 8:362–393

  • Dorn PB, Rodgers JH Jr, Jop KM, Raia JC, Dickson KL (1986) Hexavalent chromium as a reference toxicant in effluent toxicity tests. Environ Toxicol Chem 6:435–444. doi:10.1897/1552-8618(1987)6[435:HCAART]2.0.CO;2

    Article  Google Scholar 

  • Eisler R (1986) Chromium hazards to fish, wildlife and invertebrates: a synoptic review. US Fish and Wildlife Service Biological Report 85, Laurel, MD

  • Ellgaard EG, Tusa JE, Malizia AA (1978) Locomotor activity of the bluegill Lepomis macrochirus: hyperactivity induced by sublethal concentrations of cadmium, chromium and zinc. J Fish Biol 1:19–23. doi:10.1111/j.1095-8649.1978.tb04146.x

    Article  Google Scholar 

  • EPA US (1985) Ambient water quality criteria for chromium-1984. Office of Water Regulations and Standards, Washington, DC. doi:EPA-440/5-84-029

    Google Scholar 

  • EPA US (2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th edn. Office of Water, Washington, DC. doi:EPA-821-R-02-012

    Google Scholar 

  • Gendusa TC, Beitinger TL (1992) External biomarkers to assess chromium toxicity in adult Lepomis macrochirus. Bull Environ Contam Toxicol 48:237–242. doi:10.1007/BF00194377

    Article  CAS  Google Scholar 

  • Irwin RJ, Van Mouwerik M, Stevens L, Seese MD, Basham W (1997) Chromium VI (Hexavalent chromium). Environmental contaminants encyclopedia. National Park Service Water Resources Division, Fort Collins

    Google Scholar 

  • Kane AS, Salierno JD, Brewer SK (2005) Fish models in behavioral toxicology: automated techniques, updates and perspectives. In: Ostrander GK (ed) Methods in aquatic toxicology, 2nd edn. Lewis Publishers, Boca Raton, pp 559–590

    Google Scholar 

  • Scherer E (1992) Behavioural responses as indicators of environmental alterations: approaches, results, developments. J Appl Ichthyol 8:122–131. doi:10.1111/j.1439-0426.1992.tb00674.x

    Article  Google Scholar 

  • Scherer E, Harrison SE, Brown SB (1986) Locomotor activity and blood plasma parameters of acid-exposed lake whitefish (Coregonus clupeaformis). Can J Fish Aquat Sci 43:1556–1561. doi:10.1139/f86-193

    Article  CAS  Google Scholar 

  • SCORECARD (2005) Water quality indicators. http://www.scorecard.org

  • Smith JM, Heath AG (1979) Acute toxicity of copper, chromate, zinc, and cyanide to freshwater fish: effect of different temperatures. Bull Environ Contam Toxicol 22:113–119. doi:10.1007/BF02026917

    Article  CAS  Google Scholar 

  • Svecevičius G (1991) The role of olfaction in avoidance reactions to pollutants by vimba Vimba vimba (L.). Ekologija 4:3–8

    Google Scholar 

  • Svecevičius G (2005) Behavioral responses of rainbow trout Oncorhynchus mykiss to sublethal toxicity of a model mixture of heavy metals. Bull Environ Contam Toxicol 74:845–852. doi:10.1007/s00128-005-0658-3

    Article  Google Scholar 

  • Svecevičius G (2006) Acute toxicity of hexavalent chromium to European freshwater fish. Bull Environ Contam Toxicol 77:741–747 doi:10.1007/s00128-006-1126-4

    Article  Google Scholar 

  • Svecevičius G (2007) Avoidance response of rainbow trout Oncorhynchus mykiss to hexavalent chromium solutions. Bull Environ Contam Toxicol 79:596–600. doi:10.1007/s00128-007-9236-1

    Article  Google Scholar 

  • Van der Putte I, Laurier MBHM, Van Eijk (1982) Respiration and osmoregulation in rainbow trout (Salmo gairdneri) to hexavalent chromium at different pH values. Aquat Toxicol 2:99–112. doi:10.1016/0166-445X(82)90009-1

    Article  Google Scholar 

  • Van der Scalie WH, Shedd TR, Knechtges PL, Widder MW (2001) Using higher organisms in biological early warning systems for real-time toxicity detection. Biosens Bioelectron 16(7–8):457–465

    Google Scholar 

  • Van Leeuwen CJ, Hermens JLM (eds) (1995) Risk assessment of chemicals: an introduction. Kluwer Academic Publishers, Dordrecht

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  1. Laboratory of Hydrobiont Ecology and Physiology, Institute of Ecology of Vilnius University, Akademijos 2, 08412, Vilnius-21, Lithuania

    Gintaras Svecevičius

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  1. Gintaras Svecevičius
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Correspondence to Gintaras Svecevičius.

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Svecevičius, G. Use of Behavioral Responses of Rainbow Trout Oncorhynchus mykiss in Identifying Sublethal Exposure to Hexavalent Chromium. Bull Environ Contam Toxicol 82, 564–568 (2009). https://doi.org/10.1007/s00128-009-9657-0

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