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Determination of Trenbolone Residual in Bovine Liver by Liquid Chromatography–Mass Spectrometry

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Abstract

A sensitive method was developed and validated for the quantitation of trenbolone residues in bovine liver. Target analytes were extracted from liver homogenate using solid phase cartridges, separated on a Phenyl column and detected using an electrospray ionization mass spectrometer operating in positive ion mode. The mean recovery of the analytes was between 62% and 69%. The method provided detection capabilities of 1 μg/kg for trenbolone. The method is suitable for application in veterinary drug residue in surveillance programme.

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Acknowledgements

Funding support from Natural Science Foundation of China (20621703) and the Foundation of Ministry of Science & Technology of China (2002BA906A75) are gratefully acknowledged.

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Correspondence to Hai Wang.

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Wang, H., Wang, Z., Shao, B. et al. Determination of Trenbolone Residual in Bovine Liver by Liquid Chromatography–Mass Spectrometry. Bull Environ Contam Toxicol 82, 415–418 (2009). https://doi.org/10.1007/s00128-008-9589-0

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  • DOI: https://doi.org/10.1007/s00128-008-9589-0

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