Skip to main content
SpringerLink
Log in

Partial DNA sequencing of Douglas-fir cDNAs used for RFLP mapping

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

 DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) cDNA RFLP probes were determined. Sequences were submitted to the GenBank dbEST database and searched for similarity against nucleotide and protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were from plant species. Six sequences aligned with conifer genes, including genes from Douglas-fir. Similarities among the 87 sequences were revealed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA mapped markers will increase the understanding of structural gene organization of the Douglas-fir genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Pacific Southwest Research Station, USDA Forest Service, Institute of Forest Genetics, 2480 Carson Road, Placerville, CA 95667, USA, , , , , , US

    K. D. Jermstad & D. L. Bassoni

  2. Department of Environmental Horticulture, University of California, Davis, CA 95616, and Pacific Southwest Research Station, USDA Forest Service, Institute of Forest Genetics, 2480 Carson Road, Placerville, CA 95667, USA, , , , , , US

    C. S. Kinlaw & D. B. Neale

Authors
  1. K. D. Jermstad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. L. Bassoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. S. Kinlaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. B. Neale
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 10 April 1998 / Accepted: 29 April 1998

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jermstad, K., Bassoni, D., Kinlaw, C. et al. Partial DNA sequencing of Douglas-fir cDNAs used for RFLP mapping. Theor Appl Genet 97, 771–776 (1998). https://doi.org/10.1007/s001220050954

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s001220050954

Search

Navigation