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Identification of the rice D-genome chromosomes by genomic in situ hybridisation

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Abstract

 The 24 rice D-genome chromosomes were identified among the 48 chromosomes of O. latifolia, which comprise the C- and D-genomes, using genomic in situ hybridisation (GISH). The B-genome chromosomes were also discriminated from the C-genome chromosomes in O. minuta (BBCC) by GISH. A comparison of the differences in the fluorescence intensity between the C and D genomes within O. latifolia (CCDD), and between the B and C genomes within O. minuta, indicated that the overall nucleotide-sequence homology between the B and C genomes is less than that between the C and D genomes. The origin of the D genome and the phylogenetic relationship of the D genome among the rice genomes are discussed, based on the results obtained.

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Received: 5 June 1997 / Accepted: 19 June 1997

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Fukui, K., Shishido, R. & Kinoshita, T. Identification of the rice D-genome chromosomes by genomic in situ hybridisation. Theor Appl Genet 95, 1239–1245 (1997). https://doi.org/10.1007/s001220050687

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  • DOI: https://doi.org/10.1007/s001220050687

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