Skip to main content
SpringerLink
Log in

Production of transgenic eggplant (Solanum melongena L.) resistant to Colorado Potato Beetle (Leptinotarsa decemlineata Say)

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

 A modified gene of Bacillus thuringiensis var. Tolworthi (Bt), encoding a coleopteran insect-specific CryIIIB toxin, was transferred via Agrobacterium tumefaciens to the female parent of the eggplant commercial F1 hybrid ‘Rimina’. One-hundred and fifty eight transgenic plants were regenerated and tested by PCR and NPTII expression assays. The presence of the CryIIIB toxin in leaf extracts was demonstrated in 57 out of 93 transgenic plants tested by DAS-ELISA assay. High Bt-expressing plants contained a 74-kDa protein cross-reacting with serum anti-CryIIIB toxin. Twenty three out of 44 S. melongena plants tested by insect bioassay showed significant insecticidial activity on neonate larvae of Colorado Potato Beetle (CPB). The Bt transgene and the toxic effect on CPB larvae were transmitted to progenies derived by selfing. Thus, transgenic Bt eggplants represent a very effective means of CPB pest control.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Metapontum Agrobios, S.S. Jonica 106 Km 448.2, I-75010 Metaponto (MT), Italy, , , , , , IT

    S. Arpaia & F. Sunseri

  2. Istituto Sperimentale per l’Orticoltura, P.O. Box 48, I-84098 Pontecagnano (SA), Italy, , , , , , IT

    G. Mennella & V. Onofaro

  3. Istituto Sperimentale per l’Orticoltura, Via Paullese 28, I-20075 Montanaso L. (LO), Italy, , , , , , IT

    E. Perri & G. L. Rotino

Authors
  1. S. Arpaia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Mennella
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Onofaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Perri
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. Sunseri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. L. Rotino
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 25 November 1996/Accepted: 31 January 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arpaia, S., Mennella, G., Onofaro, V. et al. Production of transgenic eggplant (Solanum melongena L.) resistant to Colorado Potato Beetle (Leptinotarsa decemlineata Say). Theor Appl Genet 95, 329–334 (1997). https://doi.org/10.1007/s001220050567

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s001220050567

Search

Navigation