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RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat

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Abstract

Tan spot, caused by Pyrenophora tritici-repentis, is an economically important disease in major wheat production areas. The fungus can produce two genetically distinct symptoms on leaves of susceptible wheat genotypes: tan necrosis (nec) and extensive chlorosis (ch1). Our objectives were to determine the number of genes conditioning resistance to tan spot in a population of wheat recombinant inbred lines, and map the chromosomal location of the resistance genes using RFLPs. Conidia produced by the P. tritici-repentis isolate Pti2 (nee + chl +) were used to inoculate seedlings of 135 recombinant inbred lines derived from the cross of the synthetic hexaploid wheat W-7984 with Opata 85. A subset of the population was inoculated with conidia produced by the isolates D308 (nec − chl +) and 86–124 (nec + chl−). Inoculated seedlings were rated on a scale of 1 to 5 based on lesion type. Necrosis-inducing culture filtrate produced by the isolate 86–124 was also used to screen the entire population. A map consisting of 532 markers was employed to identify significant associations between marker loci and tan spot resistance. The entire population was insensitive to culture filtrate produced by the isolate 86–124, and the entire subset was resistant to conidial inoculation of the same isolate. The population segregated for reaction to isolates D308 and Pti2, indicating that this population segregates for resistance to extensive chlorosis only, and not to tan necrosis. RFLP analysis indicated the presence of a gene with a major effect in 1AS, a gene with a minor effect in 4AL, and an interaction between the 1AS gene and a gene in 2DL. Together, these loci explained 49.0% of the variation in this population for resistance to tan spot produced by the isolate Pti2. Two regions one in 1BL and one in 3BL, were significantly associated with resistance to extensive chlorosis, but were not significant in the multiple regression model. It should be feasible to introgress these resistance loci into adapted genetic backgrounds by using a marker-assisted selection scheme.

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Author notes

  1. J. D. Faris

    Present address: Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State Univ., 66506-5502, Manhattan, KS, USA

Authors and Affiliations

  1. Department of Plant Sciences, Loftsgard Hall, North Dakota State Univ., 58105-5051, Fargo, ND, USA

    J. D. Faris & J. A. Anderson

  2. Department of Plant Pathology, Walster Hall, North Dakota State Univ., 58105-5051, Fargo, ND, USA

    L. J. Francl & J. G. Jordahl

Authors
  1. J. D. Faris
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  2. J. A. Anderson
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  3. L. J. Francl
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  4. J. G. Jordahl
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Communicated by G. E. Hart

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Faris, J.D., Anderson, J.A., Francl, L.J. et al. RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat. Theoret. Appl. Genetics 94, 98–103 (1997). https://doi.org/10.1007/s001220050387

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  • DOI: https://doi.org/10.1007/s001220050387

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