Abstract
The level of genetic variation in 24 hop genotypes was studied using the recently developed technique for producing random amplified polymorphic DNAs (RAPDs). Of the 60 primers screened, eight produced polymorphic RAPD bands, 38 produced bands that were monomorphic for all genotypes and 19 did not produce any amplification product. It appeared that the level of polymorphism among the genotypes was generally low. Three of the primers, A11, A17 and C9, were used to determine the stability and segregation of RAPD markers in five families with a total of 182 F1 progeny. The segregation ratios of these markers in the f1 progeny suggested that they were inherited in a Mendelian manner. RAPD markers were stable and may be useful for the construction of linkage maps in hop.
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Abo-elwafa A, Murai K, Shimada T (1995) Intra- and interspecific variation in Lens revealed by RAPD markers. Theor Appl Genet 90:335–340
Arnold ML, Buckner CM, Robinson JJ (1991) Pollen-mediated introgression and hybrid speciation in Louisiana irises. Proc Natl Acad Sci USA 88:1398–1402
Carlson JE, Tulsieram LK, Glaubitz JC, Luk VWK, Kauffeldt C, Rutledge R (1991) Segregation of random amplified DNA markers in F1 progeny of conifers. Theor Appl Genet 83:194–200
Deragon JM, Landry BS (1992) RAPD and other PCR-based analyses of plant genomes using DNA extracted from small leaf disks. PCR Methods Appl 1:175–180
Devos KM, Gale MD (1992) The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567–572
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Echt CS, Erdahl LA, McCoy TJ (1992) Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome 35:84–87
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6558
Halward T, Stalker T, Larue E, Kochert G (1992) Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.). Plant Mol Biol 18:315–325
Heun M, Helentjaris T (1993) Inheritance of RAPDs in F1 hybrids of corn. Theor Appl Genet 85:961–968
Hormaza JI, Dollo L, Polito VS (1994) Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis. Theor Appl Genet 89:9–13
Hosaka K, Hanneman Jr RE (1994) Random amplified polymorphic DNA markers detected in a segregating hybrid population of Solanum chacoense x S. phureja. Jpn J Genet 69:53–66
Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511
Hunt GJ, Page RE (1992) Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphisms in the honey bee. Theor Appl Genet 85:15–20
Jarret RL, Vuylsteke DR, Gawel NJ, Pimentel RB, Dunbar LJ (1993) Detecting genetic diversity in diploid bananas using PCR and primers from a highly repetitive DNA sequence. Euphytica 68:69–76
Kazan K, Manners JM, Cameron DF (1993) Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes. Genome 36:50–56
Keil M, Griffin AR (1994) Use of random amplified polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes in Eucalyptus. Theor Appl Genet 89:442–450
Lanham PG, Brennan RM, Hackett C, McNicol RJ (1995) RAPD fingerprinting of blackcurrant (Ribes nigrum L.) cultivars. Theor Appl Genet 90:166–172
Link W, Dixkens C, Singh M, Schwall M, Melchinger AE (1995) Genetic diversity in European and Mediterranean faba bean germ plasm revealed by RAPD markers. Theor Appl Genet 90:27–32
Mailer RJ, Scarth R, Fristensky B (1994) Discrimination among cultivars of rapeseed (Brassica napus L) using DNA polymorphisms amplified from arbitrary primers. Theor Appl Genet 87:697–704
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:1212–1216
Neve RA (1976) Hops. In: Simmonds NW (ed) Evolution of crop plants. Longman, New York, pp 208–211
Neve RA (1991) Hops. Chapman and Hall, London
Ohmori T, Murata M, Motoyoshi F (1995) Identification of RAPD markers linked to the Tm-2 locus in tomato. Theor Appl Genet 90:307–311
Perron M, Gordon AG, Bousquet J (1995) Species-specific RAPD fingerprints for the closely related Picea mariana and P. rubens. Theor Appl Genet 91:142–149
Pillay M (1993) Chloroplast genome organization of brome grass, Bromus inermis Leyss. Theor Appl Genet 86:281–287
Pillay M, Kenny ST (1995) Anomalies in direct pairwise comparison of RAPDs for genetic analysis. Biotechniques 19:694–698
Rafalski JA, Tingey SV, Williams JGK (1991) RAPD markers — a new technology for genetic mapping and plant breeding. Agric Biotech News Info 3:645–648
Reed KC, Mann DA (1995) Rapid transfer of DNA agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Reiter RS, Williams JGK, Feldmann KA, Rafalski JA, Tingey SV, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs. Proc Natl Acad Sci USA 89:1477–1481
Roy A, Frascaria N, Mackay J, Bousquet J (1992) Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis. Theor Appl Genet 85:173–180
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Schnell RJ, Ronning CM, Knight Jr RJ (1995) Identification of cultivars and validation of genetic relationships in Mangifera indica L. using RAPD markers. Theor Appl Genet 90:269–274
Stiles JI, Lemme C, Sondur S, Morshidi MB, Manshardt R (1991) Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor Appl Genet 85:697–701
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technol 7:257–264
Tinker NA, Fortin MG, Mather DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet 85:976–984
Welsh J, Honeycutt RJ, McClelland M, Sobral BWS (1991) Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theor Appl Genet 82:473–476
Wilkie SE, Isaac PG, Slater RJ (1993) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor Appl Genet 86:497–504
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Wolff K, Peters-Van Rijn J (1993) Rapid detection of genetic variability in chrysanthemum (Dendranthema grandiflora Tzvelev) using random primers. Heredity 71:335–341
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Communicated by H. F. Linskens
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Pillay, M., Kenny, S.T. Random amplified polymorphic DNA (RAPD) markers in hop, Humulus lupulus: level of genetic variability and segregation in F1 progeny. Theoret. Appl. Genetics 92, 334–339 (1996). https://doi.org/10.1007/BF00223676
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DOI: https://doi.org/10.1007/BF00223676