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OsMYB103 is essential for tapetum degradation in rice

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Abstract

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OsMYB103 positively regulates tapetum degradation, and functions downstream of TDR and upstream of EAT1 and PTC1.

Abstract

The precise regulation of programmed cell death (PCD) of the tapetum is crucial for the development of anthers and pollen in rice. In this study, we isolated and identified a male-sterile mutant of rice, osmyb103, which exhibited delayed tapetum degradation and defective mature pollen. Map-based cloning and genetic complementation revealed that OsMYB103 corresponded to the gene LOC_Os04g39470 and encoded a R2R3 MYB transcription factor. OsMYB103 was localized in the nucleus and was expressed preferentially in the tapetal cells and microspores of the anther. OsMYB103 regulated the expression of two transcription factors, ETERNAL TAPETUM 1 (EAT1) and PERSISTENT TAPETAL CELL 1 (PTC1), both of which regulated tapetum degradation positively. Moreover, the expression of OsMYB103 was directly regulated by the additional positive regulator of tapetum degradation TAPETUM DEGENERATION RETARDATION (TDR) and was able to interact with it. Genetic evidence confirmed that OsMYB103 acted upstream of EAT1. The results show that OsMYB103 is a positive regulator of tapetum degradation in rice. These findings provide a better understanding of the regulatory network that underlies degradation of the tapetum in rice.

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Acknowledgements

We thank Prof. Dabing Zhang for kindly offering the tdr mutant. This work is supported by the National Natural Science Foundation of China (32072028, 31771750).

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  1. Ting Lei, Lisha Zhang and Ping Feng these authors are contributed equally to this work

Authors and Affiliations

  1. Key Laboratory of Application and Safety Control of Genetically Modified Crops, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China

    Ting Lei, Lisha Zhang, Ping Feng, Yang Liu, Wuzhong Yin, Lina Shang, Guanghua He & Nan Wang

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  1. Ting Lei
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Contributions

WN and HG designed the experiments; TL, ZL, FP, and YL performed the experiments; WZ and NS analyzed the data; TL, WN and HG wrote the paper.

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Correspondence to Guanghua He or Nan Wang.

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Lei, T., Zhang, L., Feng, P. et al. OsMYB103 is essential for tapetum degradation in rice. Theor Appl Genet 135, 929–945 (2022). https://doi.org/10.1007/s00122-021-04007-6

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