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Genetic characterization of qSCN10 from an exotic soybean accession PI 567516C reveals a novel source conferring broad-spectrum resistance to soybean cyst nematode

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Abstract

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The qSCN10 locus with broad-spectrum SCN resistance was fine-mapped to a 379-kb region on chromosome 10 in soybean accession PI 567516C. Candidate genes and potential application benefits of this locus were discussed.

Abstract

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most devastating pests of soybean, causing significant yield losses worldwide every year. Genetic resistance has been the major strategy to control this pest. However, the overuse of the same genetic resistance derived primarily from PI 88788 has led to the genetic shifts in nematode populations and resulted in the reduced effectiveness in soybean resistance to SCN. Therefore, novel genetic resistance resources, especially those with broad-spectrum resistance, are needed to develop new resistant cultivars to cope with the genetic shifts in nematode populations. In this study, a quantitative trait locus (QTL) qSCN10 previously identified from a soybean landrace PI 567516C was confirmed to confer resistance to multiple SCN HG Types. This QTL was further fine-mapped to a 379-kb region. There are 51 genes in this region. Four of them are defense-related and were regulated by SCN infection, suggesting their potential role in mediating resistance to SCN. The phylogenetic and haplotype analyses of qSCN10 as well as other information indicate that this locus is different from other reported resistance QTL or genes. There was no yield drag or other unfavorable traits associated with this QTL when near-isogenic lines with and without qSCN10 were tested in a SCN-free field. Therefore, our study not only provides further insight into the genetic basis of soybean resistance to SCN, but also identifies a novel genetic resistance resource for breeding soybean for durable, broad-spectrum resistance to this pest.

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Acknowledgements

The authors gratefully acknowledge the financial support for this study provided by the United States Department of Agriculture—National Institute of Food and Agriculture (USDA-NIFA) Grant 2019-67013-29370 and the USDA multiple state/hatch project MO-MSPS0002.

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  1. Lijuan Zhou and Li Song have contributed equally to this work.

Authors and Affiliations

  1. Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA

    Lijuan Zhou, Heng Ye, Mariola Usovsky, Jinrong Wan, Tri D. Vuong & Henry T. Nguyen

  2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, Yangzhou, 225009, China

    Li Song

  3. Institute of Industrial Crops, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Yun Lian

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  1. Lijuan Zhou
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Contributions

LZ, LS, HY, and HTN conceived and designed the project; LS and TV contributed to the development of the mapping populations; LZ, LS, YL, and MK collected plant materials and performed genotyping and phenotyping experiments. LZ and LS contributed to the data analysis; LZ wrote the manuscript; HY, TV, and JW contributed to discussion of the manuscript; and all authors critically revised the paper.

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Correspondence to Henry T. Nguyen.

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Zhou, L., Song, L., Lian, Y. et al. Genetic characterization of qSCN10 from an exotic soybean accession PI 567516C reveals a novel source conferring broad-spectrum resistance to soybean cyst nematode. Theor Appl Genet 134, 859–874 (2021). https://doi.org/10.1007/s00122-020-03736-4

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