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Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat

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Abstract

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Functional markers were developed based on the critical sequence deletion of TaHRC in the Fhb1 region and validated to be diagnostic in a worldwide wheat collection.

Abstract

Wheat Fusarium head blight (FHB) is a devastating disease in wheat and barley worldwide. Growing FHB-resistant cultivars is an effective strategy to minimize FHB damage in wheat production. Fhb1 is a quantitative trait locus for FHB resistance with the largest effect on disease severity identified to date. With this study, we developed diagnostic DNA markers for Fhb1 by comparing the genomic sequences in Fhb1 region between near-isogenic lines contrasting in Fhb1 alleles and phenotypic effects of the markers. Two markers were developed based on a deletion mutation in an gene encoding a putative histidine-rich calcium-binding protein (TaHRC) and validated in different types of populations. Haplotype or sequence analyses of the two markers in the three sets of diversity panels demonstrated that they are diagnostic for Fhb1, and superior to all previously used markers in selection accuracy. They also have the advantages of low cost, easy assay, and are suitable for breeding programs with either high- or low-throughput marker laboratories.

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Acknowledgements

This is contribution number 18-211-J from the Kansas Agricultural Experiment Station. This project is partly funded by US Wheat and Barley Scab Initiative and the National Research Initiative Competitive Grants 2011-68002-30029, 2017-67007-25939, and 2017-67007-25929 from the National Institute of Food and Agriculture, US Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035, Hebei Province, China

    Zhenqi Su

  2. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Zhenqi Su, Sujuan Jin, Dadong Zhang & Guihua Bai

  3. Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050041, China

    Sujuan Jin

  4. USDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

    Guihua Bai

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  1. Zhenqi Su
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  2. Sujuan Jin
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  3. Dadong Zhang
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  4. Guihua Bai
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Correspondence to Guihua Bai.

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The authors declared that they have no conflict of interest.

Additional information

Communicated by Thomas Miedaner.

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Su, Z., Jin, S., Zhang, D. et al. Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat. Theor Appl Genet 131, 2371–2380 (2018). https://doi.org/10.1007/s00122-018-3159-6

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  • DOI: https://doi.org/10.1007/s00122-018-3159-6

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