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A bean common mosaic virus (BCMV)-resistance gene is fine-mapped to the same region as Rsv1-h in the soybean cultivar Suweon 97

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Abstract

Key message

In the soybean cultivar Suweon 97, BCMV-resistance gene was fine-mapped to a 58.1-kb region co-localizing with the Soybean mosaic virus (SMV)-resistance gene, Rsv1-h raising a possibility that the same gene is utilized against both viral pathogens.

Abstract

Certain soybean cultivars exhibit resistance against soybean mosaic virus (SMV) or bean common mosaic virus (BCMV). Although several SMV-resistance loci have been reported, the understanding of the mechanism underlying BCMV resistance in soybean is limited. Here, by crossing a resistant cultivar Suweon 97 with a susceptible cultivar Williams 82 and inoculating 220 F2 individuals with a BCMV strain (HZZB011), we observed a 3:1 (resistant/susceptible) segregation ratio, suggesting that Suweon 97 possesses a single dominant resistance gene against BCMV. By performing bulked segregant analysis with 186 polymorphic simple sequence repeat (SSR) markers across the genome, the resistance gene was determined to be linked with marker BARSOYSSR_13_1109. Examining the genotypes of nearby SSR markers on all 220 F2 individuals then narrowed down the gene between markers BARSOYSSR_13_1109 and BARSOYSSR_13_1122. Furthermore, 14 previously established F2:3 lines showing crossovers between the two markers were assayed for their phenotypes upon BCMV inoculation. By developing six more SNP (single nucleotide polymorphism) markers, the resistance gene was finally delimited to a 58.1-kb interval flanked by BARSOYSSR_13_1114 and SNP-49. Five genes were annotated in this interval of the Williams 82 genome, including a characteristic coiled-coil nucleotide-binding site-leucine-rich repeat (CC-NBS-LRR, CNL)-type of resistance gene, Glyma13g184800. Coincidentally, the SMV-resistance allele Rsv1-h was previously mapped to almost the same region, thereby suggesting that soybean Suweon 97 likely relies on the same CNL-type R gene to resist both viral pathogens.

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Acknowledgements

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript. This research was financed by National Natural Science Foundation of China (31470327 and 31570217).

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Authors and Affiliations

  1. Laboratory of Plant Genetics and Molecular Evolution, School of Life Sciences, Nanjing University, 163 XianLin Avenue, Nanjing, 210023, China

    Mian Wu, Wen-Ping Wu, Cheng-Chen Liu, Ying-Na Liu, Xiao-Yi Wu, Fang-Fang Ma, An-Qi Zhu, Bin Wang & Jian-Qun Chen

  2. Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai’an, 223001, Jiangsu Province, China

    Jia-Yin Yang

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  1. Mian Wu
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  2. Wen-Ping Wu
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Correspondence to Jia-Yin Yang, Bin Wang or Jian-Qun Chen.

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Communicated by Brian Diers.

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Fig. S1

Phenotypes of resistant and susceptible F2 individuals upon inoculation by BCMV-HZZB011 in the cross Suweon 97 × Williams 82. The appearance of soybean plants after 3 to 4 weeks inoculation by HZZB011 is shown in A (left, resistant; right, susceptible). A trifoliate leaf (B) derived from resistant recombinant offspring of the cross Suweon 97 × Williams 82 that shows no disease symptoms. Panel C shows classical mosaic symptoms and D show deformed leaves in the susceptible recombinant offspring. E and F showed the old leaves from the resistant and susceptible plants, respectively. (PDF 5638 kb)

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Wu, M., Wu, WP., Liu, CC. et al. A bean common mosaic virus (BCMV)-resistance gene is fine-mapped to the same region as Rsv1-h in the soybean cultivar Suweon 97. Theor Appl Genet 131, 1851–1860 (2018). https://doi.org/10.1007/s00122-018-3117-3

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