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Fine genetic mapping of target leaf spot resistance gene cca-3 in cucumber, Cucumis sativus L.

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The cucumber target leaf spot resistance gene cca - 3 was fine mapped in a 79-kb region harboring a CC-NB-ARC type R gene that may be responsible for the hypersensitive responses to infection of the target leaf spot pathogen in cucumber.

Abstract

The target leaf spot (TLS) is one of the most important foliar diseases in cucumber (Cucumis sativus L.). In this study, we conducted fine genetic mapping of a simply inherited recessive resistance gene, cca-3 against TLS with 193 F2:3 families and 890 F2 plants derived from the resistant cucumber inbred line D31 and the susceptible line D5. Initial mapping with microsatellite markers and bulked segregant analysis placed cca-3 in a 2.5-Mbp region of cucumber chromosome 6. The D5 and D31 lines were re-sequenced at 10× genome coverage to explore new markers in the target region. Genetic mapping in the large F2 population delimited the cca-3 locus in a 79-kb region with flanking markers Indel16874230 and Indel16953846. Additional fine mapping and gene annotation in this region revealed that a CC-NB-ARC type resistance gene analog, Csa6M375730, seems to be the candidate gene for cca-3. One single nucleotide polymorphism (SNP) was found in the NB-ARC domain of this candidate gene sequence between D31 and D5 that may lead to amino acid change, thus altering the function of the conserved NB-ARC motif. This SNP was validated in the segregating population as well as 24 independent cucumber lines. There was significantly higher level of cca-3 expression in the leaves of D5 (susceptible) than in D31 (resistant), and the expression level was positively correlated with the areas of necrotic spots on leaves after inoculation. It seems the cca-3 resistance gene was able to induce hypersensitive responses to the infection by TLS pathogen.

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Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (No. 31401873 to CW), the National Science & Technology Pillar Program (2014BAD01B09 and 2012BAD02B03), the National High Technology Research and Development Program of China (2012AA100103), the Beijing Municipal Science and Technology Commission(Z131100003113012), and Beijing Academy of Agricultural and Forestry Sciences (CXJJ201305/QNJJ201501).

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    Authors and Affiliations

    1. National Engineering Research Center for Vegetables, Beijing Vegetable Research Center (BVRC), Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China

      Changlong Wen, Aijun Mao, Congjuan Dong, Huyu Liu, Shuancang Yu & Yong Xu

    2. Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing, 100097, China

      Changlong Wen, Aijun Mao, Congjuan Dong, Huyu Liu, Shuancang Yu & Yong Xu

    3. College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, China

      Yang-Dong Guo

    4. USDA-ARS, Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI, 53706, USA

      Yiqun Weng

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    1. Changlong Wen
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    Correspondence to Changlong Wen.

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    Communicated by C. Gebhardt.

    C. Wen, A. Mao, and C. Dong contributed equally to this work.

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    Wen, C., Mao, A., Dong, C. et al. Fine genetic mapping of target leaf spot resistance gene cca-3 in cucumber, Cucumis sativus L.. Theor Appl Genet 128, 2495–2506 (2015). https://doi.org/10.1007/s00122-015-2604-z

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