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Tetralocular ovary and high silique width in yellow sarson lines of Brassica rapa (subspecies trilocularis) are due to a mutation in Bra034340 gene, a homologue of CLAVATA3 in Arabidopsis

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Genetic locus for tetralocular ovary ( tet - o ) in Brassica rapa was identified and it was shown that the number of locules and width of silique are associated.

Abstract

Brassica rapa is a highly polymorphic species containing many vegetables and oleiferous types. An interesting group of oleiferous types is the yellow sarson group (subspecies trilocularis) grown mostly in eastern India. This group contains lines that have bilocular ovaries, a defining trait of Brassicaceae, but also lines that have tetralocular ovaries. Yellow sarson lines commonly have high silique width which is further enhanced in the tetralocular types. We mapped the locus influencing tetralocular ovary in B. rapa using three mapping populations (F2, F6 and F7) derived from a cross between Chiifu (subspecies pekinensis, having bilocular ovary) and Tetralocular (having tetralocular ovary). QTL mapping of silique width was undertaken using the three mapping populations and a F2 population derived from a cross between Chiifu and YSPB-24 (a bilocular line belonging to yellow sarson group). Qualitative mapping of the trait governing locule number (tet-o) in B. rapa mapped the locus to linkage group A4. QTL mapping for silique width detected a major QTL on LG A4, co-mapping with the tet-o locus in bilocular/tetralocular cross. This QTL was not detected in the bilocular/bilocular cross. Saturation mapping of the tet-o region with SNP markers identified Bra034340, a homologue of CLAVATA3 of Arabidopsis thaliana, as the candidate gene for locule number. A C → T transition at position 176 of the coding sequence of Bra034340 revealed co-segregation with the tetralocular phenotype. The study of silique related traits is of interest both for understanding evolution under artificial selection and for breeding of cultivated Brassica species.

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Acknowledgments

The study was funded by JC Bose fellowship (Department of Science and Technology, Government of India) to Deepak Pental and also partly supported by the National Dairy Development Board and the Department of Biotechnology, Government of India through the award of a Centre of Excellence on Brassica breeding. Technical support provided by Meena Bhandari, Simi Pahwa and J K Verma is duly acknowledged.

Conflict of interest

The authors declare that they have no competing interests.

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The manuscript entitled “Tetralocular ovary and high silique width in yellow sarson lines of Brassica rapa (subspecies trilocularis) are due to a mutation in Bra034340 gene, a homologue of CLAVATA3 in Arabidopsis” being submitted for publication in Theoretical and Applied Genetics complies with the current laws of India for academic and research purposes.

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  1. Priya Panjabi-Massand

    Present address: Department of Botany, University of Delhi, Delhi, 110007, India

  2. Atika Chandra

    Present address: Department of Botany, Maitreyi College, University of Delhi, New Delhi, 110021, India

Authors and Affiliations

  1. Centre for Genetic Manipulation of Crop Plants, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Satish Kumar Yadava, Kumar Paritosh, Priya Panjabi-Massand, Vibha Gupta, Atika Chandra, Y. S. Sodhi, Akshay K. Pradhan & Deepak Pental

  2. Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Akshay K. Pradhan & Deepak Pental

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  1. Satish Kumar Yadava
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Correspondence to Deepak Pental.

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Communicated by Lixi Jiang.

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Yadava, S.K., Paritosh, K., Panjabi-Massand, P. et al. Tetralocular ovary and high silique width in yellow sarson lines of Brassica rapa (subspecies trilocularis) are due to a mutation in Bra034340 gene, a homologue of CLAVATA3 in Arabidopsis. Theor Appl Genet 127, 2359–2369 (2014). https://doi.org/10.1007/s00122-014-2382-z

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