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An intra-specific linkage map of lettuce (Lactuca sativa) and genetic analysis of postharvest discolouration traits

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Abstract

Minimally processed salad packs often suffer from discolouration on cut leaf edges within a few days after harvest. This limits shelf life of the product and results in high wastage. Recombinant inbred lines (RILs) derived from a cross between lettuce cvs. Saladin and Iceberg were shown to be suitable for genetic analysis of postharvest discolouration traits in lettuce. An intra-specific linkage map based on this population was generated to enable genetic analysis. A total of 424 markers were assigned to 18 linkage groups covering all nine chromosomes. The linkage map has a total length of 1,040 cM with an average marker distance of 2.4 cM within the linkage groups and was anchored to the ultra-dense, transcript-based consensus map. Significant genetic variation in the postharvest traits ‘pinking’, ‘browning’ and ‘overall discolouration’ was detected among the RILs. Seven significant quantitative trait loci (QTL) were identified for postharvest discolouration traits providing markers linked to the QTL that can be used for marker-assisted selection. Phenotypic stability was confirmed for extreme lines possessing the corresponding QTL parental alleles and which had shown transgressive segregation. This study indicates that a desired phenotype with reduced levels of postharvest discolouration can be achieved by breeding using natural variation.

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Acknowledgments

The research was primarily conducted at the University of Warwick, Wellesbourne campus, formerly Warwick HRI. We would like to thank a number of laboratory personnel at Warwick HRI, in particular, Sandy McClement and Neale Grant who helped in the planting and maintaining of the field trials in the UK. Rijk Zwaan Ltd. maintained the field site in the Netherlands and we would like to specifically thank Wendy Deijkers for help during harvest and data collection. This work was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) Crop Science Initiative CASE PhD studentship (BBS/S/E/2006/13221) for LDA in collaboration with Rijk Zwaan Ltd, EU GenRes project “Leafy vegetables germplasm, stimulating use” and Department for Environment, Food and Rural Affairs (Defra) project IF0157 “Vegetable Genetic Improvement Network (VeGIN): Pre-breeding research to support sustainable farming of leafy vegetables and salads”. Travel and subsistence for LDA to work at UC Davis was provided by grants from the Vegetable Research Trust, Glasshouse Crop Research Institute Trust, UK Resource Centre for Women in Science, Engineering and Technology, American Study and Student Exchange Committee and a Warwick HRI Student Travel Award. The contributions of LKH, MJT, and RWM were part of the Compositae Genome Project and supported by the National Science Foundation Plant Genome Program Grant # DBI0421630.

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Author notes

  1. Laura D. Atkinson

    Present address: Monsanto UK Ltd, P.O. Box 663, Cambridge, CB1 0LD, UK

  2. Leah K. McHale

    Present address: Department of Horticulture and Crop Science, The Ohio State University, 2021 Coffey Road, Columbus, OH, 43210, USA

  3. Howard W. Hilton

    Present address: SGS UK Ltd, Meadow View, Alkerton Oaks Business Park, Stratford Road, Upton Estate, Banbury, Oxfordshire, OX15 6EP, UK

  4. James Lynn

    Present address: Applied Statistical Solutions, 10 Church Hill, Bishops Tachbrook, Leamington Spa, Warwickshire, CV33 9RJ, UK

  5. Paul Hand & David A. C. Pink

    Present address: Crop and Environment Science Department, Harper-Adams University, Newport, Shropshire, TF10 8NB, UK

Authors and Affiliations

  1. Department of Plant Sciences, Genome Center, University of California, Davis, CA, 95616, USA

    María José Truco & Richard W. Michelmore

  2. Rijk Zwaan Zaadteelt en Zaadhandel B.V, P.O. Box 40, 2678, De Lier, The Netherlands

    Johan W. Schut

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  1. Laura D. Atkinson
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Correspondence to David A. C. Pink.

Additional information

Communicated by M. Havey.

Electronic supplementary material

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122_2013_2168_MOESM1_ESM.docx

Online Resource 1 Mapped IGG marker name conversions from the Saladin × Iceberg linkage map. Marker information for the Illumina GoldenGate assay available at http://chiplett.ucdavis.edu/public/Data/MAP_214RILs/map_2009/index.php (DOCX 23 kb)

122_2013_2168_MOESM2_ESM.docx

Online Resource 2 Mapped SPP marker sequences from the Saladin × Iceberg linkage map. Where GeneChip Sequence Assembly ID is the EST/Contig retrieved from http://cgpdb.ucdavis.edu/cgpdb2/CGP_ContigViewer/ and the SPP Position is single positional polymorphism location in that sequence (DOCX 11 kb)

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Atkinson, L.D., McHale, L.K., Truco, M.J. et al. An intra-specific linkage map of lettuce (Lactuca sativa) and genetic analysis of postharvest discolouration traits. Theor Appl Genet 126, 2737–2752 (2013). https://doi.org/10.1007/s00122-013-2168-8

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