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Genetic variability and QTL mapping of freezing tolerance and related traits in Medicago truncatula

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Abstract

Freezing is a major environmental limitation to crop productivity for a number of species including legumes. We investigated the genetic determinism of freezing tolerance in the model legume Medicago truncatula Gaertn (M. truncatula). After having observed a large variation for freezing tolerance among 15 M. truncatula accessions, the progeny of a F6 recombinant inbred line population, derived from a cross between two accessions, was acclimated to low above-freezing temperatures and assessed for: (a) number of leaves (NOL), leaf area (LA), chlorophyll content index (CCI), shoot and root dry weights (SDW and RDW) at the end of the acclimation period and (b) visual freezing damage (FD) during the freezing treatment and 2 weeks after regrowth and foliar electrolyte leakage (EL) 2 weeks after regrowth. Consistent QTL positions with additive effects for FD were found on LG1, LG4 and LG6, the latter being the most explanatory (R 2 ≈ 40 %). QTL for NOL, QTL for EL, NOL and RDW, and QTL for EL and CCI colocalized with FD QTL on LG1, LG4 and LG6, respectively. Favorable alleles for these additive effects were brought by the same parent suggesting that this accession contributes to superior freezing tolerance by affecting plants’ capacity to maintain growth at low above-freezing temperatures. No epistatic effects were found between FD QTL, but for each of the studied traits, 3–6 epistatic effects were detected between loci not detected directly as QTL. These results open the way to the assessment of syntenic relationships between QTL for frost tolerance in M. truncatula and cultivated legume species.

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Acknowledgments

We are very grateful to M. Delalande from INRA center of Mauguio (Montpellier, France) for kindly providing seeds of the M. truncatula LR3 population. We are also grateful to F. Depta, J-F. Hu, B. Decaux, R. Devaux, M. Boilleau, O. Jaminon, A-S. Niquet, K. Lourgant, A. Ketele, G. Deniot and I. Le Goff for either their technical support for devices used or great help for periodical sampling. This work was supported by the Picardie region and by the UNIP (Union Nationale Interprofessionnelle des Plantes Riches en Protéines). The development of the F83005-5 × DZA045-5 genetic map was supported within the EU-FP6 GLIP project. We thank two anonymous referees and the editor of this paper for their helpful and constructive comments.

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  1. Komlan Avia

    Present address: Plant Genetics Group, Department of Biology, University of Oulu, PO Box 3000, 90014, Oulu, Finland

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  1. INRA, UMR 1281 SADV, Estrées-Mons, BP 50136, 80203, Péronne Cedex, France

    Komlan Avia, Nasser Bahrman, Véronique Fontaine, Eric Hanocq, Martine Niarquin, Hélène Sellier & Isabelle Lejeune-Hénaut

  2. INRA, UMR 1349 IGEPP, Domaine de la Motte, BP 35327, 35653, Le Rheu Cedex, France

    Marie-Laure Pilet-Nayel, Alain Baranger & Céline Hamon

  3. USTL, UMR 1281 SADV, Bâtiment SN2, 59655, Villeneuve d’Ascq, France

    Bruno Delbreil & Christophe Vuylsteker

  4. INRA, UMR 1334 AGAP, 2 Place Viala, 34060, Montpellier Cedex 2, France

    Jean-Marie Prosperi

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Avia, K., Pilet-Nayel, ML., Bahrman, N. et al. Genetic variability and QTL mapping of freezing tolerance and related traits in Medicago truncatula . Theor Appl Genet 126, 2353–2366 (2013). https://doi.org/10.1007/s00122-013-2140-7

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