Abstract
The partially dominant, autoactive maize disease resistance gene Rp1-D21 causes hypersensitive response (HR) lesions to form spontaneously on leaves and stems in the absence of pathogen recognition. The maize nested association mapping (NAM) population consists of 25 200-line subpopulations each derived from a cross between the maize line B73 and one of 25 diverse inbred lines. By crossing a line carrying the Rp1-D21 gene with lines from three of these subpopulations and assessing the F1 progeny, we were able to map several novel loci that modify the maize HR, using both single-population quantitative trait locus (QTL) and joint analysis of all three populations. Joint analysis detected QTL in greater number and with greater confidence and precision than did single population analysis. In particular, QTL were detected in bins 1.02, 4.04, 9.03, and 10.03. We have previously termed this technique, in which a mutant phenotype is used as a “reporter” for a trait of interest, Mutant-Assisted Gene Identification and Characterization (MAGIC).
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Acknowledgments
This work was funded by USDA-ARS, Purdue University and an NSF Grant # 0822495. We thank Jim Holland for donating seed and David Rhyne, Abbey Sutton, Joe Bundy, Donna Stephens, Sandeep Marla and Kevin Chu for help with fieldwork.
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Communicated by T. Luebberstedt.
V. Chaikam and A. Negeri made equal contributions to the work.
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Chaikam, V., Negeri, A., Dhawan, R. et al. Use of mutant-assisted gene identification and characterization (MAGIC) to identify novel genetic loci that modify the maize hypersensitive response. Theor Appl Genet 123, 985–997 (2011). https://doi.org/10.1007/s00122-011-1641-5
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DOI: https://doi.org/10.1007/s00122-011-1641-5