Abstract
Brassica napus, an allopolyploid species having the A genome of B. rapa and the C genome of B. oleracea, is self-compatible, although both B. rapa and B. oleracea are self-incompatible. We have previously reported that SP11/SCR alleles are not expressed in anthers, while SRK alleles are functional in the stigma in B. napus cv. ‘Westar’, which has BnS-1 similar to B. rapa S-47 and BnS-6 similar to B. oleracea S-15. This genotype is the most frequent S genotype in B. napus, and we hypothesized that the loss of the function of SP11 is the primary cause of the self-compatibility of ‘Westar’. To verify this hypothesis, we transformed ‘Westar’ plants with the SP11 allele of B. rapa S-47. All the transgenic plants and their progeny were completely self-incompatible, demonstrating self-compatibility to be due to the S haplotype having the non-functional SP11 allele in the A genome, which suppresses a functional recessive SP11 allele in the C genome. An artificially synthesized B. napus line having two recessive SP11 alleles was developed by interspecific hybridization between B. rapa and B. oleracea. This line was self-incompatible, but F1 hybrids between this line and ‘Westar’ were self-compatible. These results suggest that the self-compatibility mechanism of ‘Westar’ is applicable to F1 seed production in B. napus.
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This work was supported in part by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).
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122_2011_1600_MOESM1_ESM.pdf
Supplementary Fig. 1. Genomic Southern-blot analysis of transgenic plants using a probe of BrSP11-47. Genomic DNAs of transgenic plants (1: TSP11-47-1, 2: TSP11-47-2, 3: TSP11-47-3) were digested with HindIII (left) or SacI (right). Bands different from those of ‘Westar’ are of transgenes (PDF 47 kb)
122_2011_1600_MOESM2_ESM.pdf
Supplementary Fig. 2. GUS activity in pollen grains of a transgenic plant with the BrSP11-47 promoter::GUS transgene. Pollen grains of 4, 5, and 10-mm-long buds were stained by X-gluc (PDF 161 kb)
122_2011_1600_MOESM5_ESM.pdf
Supplementary Fig. 5. Amounts of transcripts of BrSP11-47 analyzed by real-time RT-PCR in T1 plants. Relative values are shown with a standard of BrS-47 (= 1). Measurements were repeated three times, and bars indicate SE (PDF 46 kb)
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Tochigi, T., Udagawa, H., Li, F. et al. The self-compatibility mechanism in Brassica napus L. is applicable to F1 hybrid breeding. Theor Appl Genet 123, 475–482 (2011). https://doi.org/10.1007/s00122-011-1600-1
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DOI: https://doi.org/10.1007/s00122-011-1600-1