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Fine mapping of qSTV11 TQ, a major gene conferring resistance to rice stripe disease

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Abstract

The indica rice cultivar, Teqing, shows a high level of resistance to rice stripe virus (RSV). It is believed that this resistance is controlled by the gene, qSTV11 TQ. For positional cloning of the resistance gene, a set of chromosome single segment substitution lines (CSSSLs) was constructed, all of which had the genetic background of the susceptible japonica cultivar, Lemont, with different single substituted segments of Teqing on chromosome 11. By identifying the resistance of the CSSSLs-2006 in a field within a heavily diseased area, the resistance gene qSTV11 TQ was mapped between the markers Indel7 and RM229. Furthermore, in that region, six new markers were developed and 52 subregion CSSSLs (CSSSLs-2007) were constructed. The natural infection experiment was conducted again at different sites, with two replicates used in each site in order to identify the resistance phenotypes of the CSSSLs-2007 and resistant/susceptible controls in 2007. Through the results of 2007, qSTV11 TQ was localized in a region defined by the markers, CAPs1 and Indel4. In order to further confirm the position of qSTV11 TQ, another set of subregion CSSSLs (CSSSLs-2009) was constructed. Finally, qSTV11 TQ was localized to a 55.7 kb region containing nine annotated genes according to the genome sequence of japonica Nipponbare. The relationship between qSTV11 TQ and Stvb-i (Hayano-Saito et al. in Theor Appl Genet 101:59–63, 2000) and the reliability of the markers used on both sides of qSTV11 TQ for marker-assisted breeding of resistance to rice stripe disease are discussed.

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Acknowledgments

We would like to thank Dr. Aihong Li, Dr. Cunhong Pan and Mr. Zongliang Chen for their pertinent suggestions and great help in field management. This work was partially supported by the grants from the ‘863’ High Technology Project of China (2007AA10Z191) and the Key Project of Transgenic Rice Cultivation of the Ministry of Agriculture of China (2008ZX08001-002).

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Authors and Affiliations

  1. Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Xujiang Wu, Shimin Zuo, Zongxiang Chen, Yafang Zhang, Junkai Zhu, Ning Ma & Xuebiao Pan

  2. Key Laboratory for Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Xujiang Wu, Shimin Zuo, Zongxiang Chen, Yafang Zhang, Junkai Zhu, Ning Ma & Xuebiao Pan

  3. National Plant Gene Research Center, Institute of Genetics and Development Biology, Chinese Academy of Science, Beijing, 100101, People’s Republic of China

    Jiuyou Tang & Chengcai Chu

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  1. Xujiang Wu
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  2. Shimin Zuo
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  3. Zongxiang Chen
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  4. Yafang Zhang
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  5. Junkai Zhu
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  6. Ning Ma
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  7. Jiuyou Tang
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  8. Chengcai Chu
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  9. Xuebiao Pan
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Correspondence to Xuebiao Pan.

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Communicated by L. Xiong.

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Wu, X., Zuo, S., Chen, Z. et al. Fine mapping of qSTV11 TQ, a major gene conferring resistance to rice stripe disease. Theor Appl Genet 122, 915–923 (2011). https://doi.org/10.1007/s00122-010-1498-z

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  • DOI: https://doi.org/10.1007/s00122-010-1498-z

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