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Identification of QTL regions and SSR markers associated with resistance to reniform nematode in Gossypium barbadense L. accession GB713

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Abstract

The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford & Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers to specific chromosomes. We grew and scored plants for RN reproduction in the P1, P2, F1, F2, BC1P1, and BC1P2 generations from the cross of GB713 × Acala Nem-X. The generation means analysis using the six generations indicated that one or more genes were involved in the RN resistance of GB713. The interspecific F2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton (G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position 168.6 (LOD 28.0) flanked by SSR markers, BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions 180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions 27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Ren barb1 and Ren barb2 to designate these QTLs on chromosome 21 and Ren barb3 on chromosome 18.

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Abbreviations

cM:

Centimorgans

MAS:

Marker-assisted selection

SSRs:

Simple sequence repeats

RN:

Reniform nematode

QTL:

Quantitative trait loci

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Acknowledgments

The authors would like to express thanks to Mr. Douglas Dollar for his technical assistance. In addition, the authors want to express their gratitude to Dr. Alois A. Bell for his fruitful discussion regarding the inheritance of RN resistance. This research was partially funded by a cooperative research agreement from Cotton Incorporated Breeding and Genetics Initiative Program. Contribution of the Mississippi Agricultural and Forestry Experiment Station, in cooperation with the USDA-ARS. Approved for publication as Journal Article No. J-11873 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University. Mention of a trademark, warranty, proprietary product or vendor does not constitute a guarantee by Mississippi State University or the US Department of Agriculture and does not imply approval or recommendation of the product to the exclusion of others that may be suitable.

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Authors and Affiliations

  1. Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS, 39762-5367, USA

    Osman A. Gutiérrez

  2. USDA-ARS, Cotton Pathology Research, College Station, TX, 77843-2474, USA

    Arin F. Robinson

  3. Genetics and Precision Agriculture Research Unit, Crop Science Research Laboratory, USDA-ARS, Mississippi State, MS, 39762-5367, USA

    Johnie N. Jenkins, Jack C. McCarty, Martin J. Wubben & Franklin E. Callahan

  4. Cotton Incorporated, 6399 Weston Parkway, Cary, NC, 27513, USA

    Robert L. Nichols

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  1. Osman A. Gutiérrez
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  2. Arin F. Robinson
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Correspondence to Osman A. Gutiérrez.

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Communicated by D. Mather.

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Gutiérrez, O.A., Robinson, A.F., Jenkins, J.N. et al. Identification of QTL regions and SSR markers associated with resistance to reniform nematode in Gossypium barbadense L. accession GB713. Theor Appl Genet 122, 271–280 (2011). https://doi.org/10.1007/s00122-010-1442-2

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