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Identification of a novel gene ef7 conferring an extremely long basic vegetative growth phase in rice

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Abstract

A late heading-time mutant line, HS276, which was induced by gamma-irradiation of seeds of the japonica rice (Oryza sativa L.) variety Gimbozu, exhibits an extremely long basic vegetative growth phase (BVP). A genetic analysis using the F2 population from the cross between HS276 and Gimbozu revealed that the late heading of HS276 is governed by a single recessive mutant gene. The subsequent analysis on heading responses of HS276 and Gimbozu to four photoperiods (12, 13, 14, and 15 h) and to the photoperiodic transfer treatment from a short photoperiod to a long photoperiod revealed that the mutant gene confers an extremely long BVP and increases photoperiod sensitivity under long photoperiod (14 and 15 h). The BVP durations of HS276 and Gimbozu were estimated at 30.1 and 16.0 days, respectively; the mutant gene, compared with its wild type allele, elongates the duration of BVP by 14 days. Linkage analysis showed that the mutant gene is located in the 129 kb region between the two INDEL markers, INDELAP0399_6 and INDELAP3487_2, on the distal part of the short arm of chromosome 6. None of the other BVP genes are located in this region; therefore, we declared this a newly detected mutant gene and designated it ef7. A recently established program to breed rice suitable for low latitudes, where short photoperiodic conditions continue throughout the year, aims to develop varieties with extremely long BVPs and weak photoperiod sensitivities; the mutant gene ef7, therefore, will be quite useful in these programs because it confers an extremely long BVP and little enhances photoperiod sensitivity under short photoperiod.

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Acknowledgments

We thank the Rice Genome Resource Center (RGRC) (Tsukuba, Japan) for providing the seeds of chromosome segment substitution lines. This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Integrated research project for plant, insect and animal using genome technology QTL-4001).

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Authors and Affiliations

  1. Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyou, Kyoto, 606-8502, Japan

    Qingbo Yuan, Hiroki Saito, Yutaka Okumoto, Hiromo Inoue, Takuji Tsukiyama, Masayoshi Teraishi & Takatoshi Tanisaka

  2. Faculty of Agriculture, Okayama University, 1-1-1 Tsushima Naka, Okayama, 700-8530, Japan

    Hidetaka Nishida

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  1. Qingbo Yuan
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  2. Hiroki Saito
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  3. Yutaka Okumoto
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  6. Takuji Tsukiyama
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  7. Masayoshi Teraishi
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  8. Takatoshi Tanisaka
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Correspondence to Takatoshi Tanisaka.

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Communicated by J. Snape.

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Yuan, Q., Saito, H., Okumoto, Y. et al. Identification of a novel gene ef7 conferring an extremely long basic vegetative growth phase in rice. Theor Appl Genet 119, 675–684 (2009). https://doi.org/10.1007/s00122-009-1078-2

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  • DOI: https://doi.org/10.1007/s00122-009-1078-2

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