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Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.)

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Abstracts

Chalkiness of rice grain is an important quality component of rice, as it has a profound influence on eating and milling qualities. We has determined the inheritance of percentage of grain with chalkiness (PGWC) using a set of chromosome segment substitution lines, made from a cross between cv. PA64s and cv. 9311. Two loci controlling PGWC, designated as qPGWC-6 and qPGWC-7, were located on, respectively, chromosomes 6 and 7. Comparisons were made between C-51 (a CSSL harbouring qPGWC-7 and having a chalky endosperm) and the recurrent parent 9311 (translucent endosperm) to characterize the physical and chemical differences between translucent and chalky endosperm. Unlike the translucent endosperm, the chalky endosperm contains loosely packed starch granules, and there were significant difference between C-51 and 9311 for amylopectin structure and degree of crystallinity, but not for either amylose content or starch viscosity. Segregation analysis of the F2 population from the cross between C-51 and 9311 showed PGWC is a semi-dominant trait, controlled by single nuclear gene. A large F2 population was constructed from the cross C51 × 9311, and used for the fine mapping of qPGWC-7, which was located to a 44-kb DNA fragment, containing thirteen predicted genes. This result provides a springboard for the map-based cloning of qPGWC-7 and allowed for marker-assisted selection for endosperm texture.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30500315), 863 Program of China (2006AA10Z1A5, 2006AA100101, 2007AA10Z116), National Science and Technology Supporting Program (2006BAD13B01), Jiangsu Province Agricultural Germplasm Gene Pool Program (sx(2007)g02) and the 111 project (B08025).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, 210095, Nanjing, China

    Lijun Zhou, Liangming Chen, Ling Jiang, Wenwei Zhang, Linglong Liu, Xi Liu, Zhigang Zhao, Shijia Liu, Lujun Zhang & Jianmin Wan

  2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Jiankang Wang & Jianmin Wan

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  1. Lijun Zhou
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Correspondence to Jianmin Wan.

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Communicated by M. Morgante.

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Zhou, L., Chen, L., Jiang, L. et al. Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.). Theor Appl Genet 118, 581–590 (2009). https://doi.org/10.1007/s00122-008-0922-0

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  • DOI: https://doi.org/10.1007/s00122-008-0922-0

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