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Identification of candidate signaling genes including regulators of chromosome condensation 1 protein family differentially expressed in the soybean–Phytophthora sojae interaction

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Abstract

Stem and root rot caused by the oomycete pathogen, Phytophthora sojae, is a serious soybean disease. Use of Phytophthora resistance genes (Rps) in soybean cultivars has been very effective in controlling this pathogen. Resistance encoded by Rps genes is manifested through activation of defense responses. In order to identify candidate signaling genes involved in the expression of Phytophthora resistance in soybean, a cDNA library was prepared from infected etiolated hypocotyl tissues of a Phytophthora resistant soybean cultivar harvested 2 and 4 h following P. sojae inoculation. In silico subtraction of 101,833 expressed sequence tags (ESTs) originating from unstressed cDNA libraries from 4,737 ESTs of this library resulted in identification of 204 genes that were absent in the unstressed libraries. Of the 204 identified genes, seven were P. sojae genes. Putative function of 91 of the 204 genes could not be assigned based on sequence comparison. Macroarray analyses of all 204 genes led to identification of 60 genes including 15 signaling-related soybean genes and three P. sojae genes, transcripts of which were induced twofold in P. sojae-infected tissues as compared to that in water controls. Eight soybean genes were down-regulated twofold following P. sojae infection as compared to water controls. Differential expression of a few selected genes was confirmed by conducting Northern and RT-PCR analyses. We have shown that two putative regulators of chromosome condensation 1 (RCC1) family proteins were down-regulated in the incompatible interaction. This observation suggested that the nucleocytoplasmic transport function for trafficking protein and non-coding RNA is suppressed during expression of race-specific Phytophthora resistance. Characterization of a cDNA library generated from tissues harvested almost immediately following P. sojae-infection of a resistant cultivar allowed us to identify many candidate signaling genes that are presumably involved in regulating the expression of defense-related pathways for expression of Phytophthora resistance in soybean.

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Acknowledgments

This research was funded by USDA-NRI (Grant No. 2001-35301-10577) and Iowa Soybean Association. Technical assistance by Mr. Datta Prasad Kamat is highly appreciated.

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  1. Narayanan N. Narayanan

    Present address: Department of Plant Cellular and Molecular Biology, The Ohio State University, Columbus, OH, 43210, USA

Authors and Affiliations

  1. Department of Agronomy, Iowa State University, Ames, IA, 50011, USA

    Narayanan N. Narayanan, Sehiza Grosic, I. M. Tasma & Madan K. Bhattacharyya

  2. USDA-ARS-CICGR, Ames, IA, 50011, USA

    David Grant & Randy Shoemaker

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  1. Narayanan N. Narayanan
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Correspondence to Madan K. Bhattacharyya.

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Communicated by E. Guiderdoni.

N. N. Narayanan and M. K. Bhattacharya equally contributed to the article.

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Narayanan, N.N., Grosic, S., Tasma, I.M. et al. Identification of candidate signaling genes including regulators of chromosome condensation 1 protein family differentially expressed in the soybean–Phytophthora sojae interaction. Theor Appl Genet 118, 399–412 (2009). https://doi.org/10.1007/s00122-008-0895-z

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