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Analysis of introgression of Aegilops ventricosa Tausch. genetic material in a common wheat background using C-banding

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Abstract

Seven Triticum aestivum (cv. Moisson)–Aegilops ventricosa addition lines and four VPM-1 lines were studied by C-banding, and compared with the parental common wheat cultivars Marne-Desprez (hereafter Marne), Moisson, and A. ventricosa lines 10 and 11. All of the VPM-1 lines had similar C-banding patterns and carried the same major 5B:7B translocation as the parental Marne cultivar. According to the C-banding analysis, the VPM-1 lines carry a complete 7D(7Dv) chromosome substitution and a translocation involving the 5D and 5Dv chromosomes. However, the translocation of the 2Nv/6Nv chromosome of A. ventricosa to the short arm of the 2A chromosome of wheat that had been identified in an earlier study using molecular analysis (Bonhomme A, Gale MD, Koebner RMD, Nicolas P, Jahier J, Bernard M in Theor Appl Genet 90:1042–1048, 1995; Jahier J, Abelard P, Tanguy AM, Dedryver F, Rivoal R, Khatkar S, Bariana HS Plant Breed 120:125–128, 2001) was not detected in our study. However, the appearance of a small pAs1 site at the tip of the chromosome 2A short arm in VPM-1 could be indicative of a minor translocation of the A. ventricosa chromosome. The 5B:7B translocation was also found in all seven T. aestivum–A. ventricosa addition lines, although it was not present in the parental common wheat cultivar Moisson. These lines showed different introgression patterns; besides the addition of the five Nv-genome chromosomes, they also possessed different D(Dv) genome substitutions or translocations. A whole arm translocation between chromosome 1Nv and 3Dv was identified in lines v86 and v137, and also in the A. ventricosa line 10. This observation lends further support to the idea that A. ventricosa line 10, rather than line 11, was used to develop a set of wheat A. ventricosa addition lines.

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Acknowledgments

The authors would like to thank Dr J. Jahier (INRA-Rennes, France) for kindly providing the material for investigation. This work was supported by a grant from the Russian State Foundation for Basic Research (project # 08-04-00302) and an INRA-MRI grant for Franco-Russian cooperation.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow, 119991, Russia

    E. D. Badaeva

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow, 119991, Russia

    O. S. Dedkova

  3. INRA, Plant Breeding and Health, UMR1095 INRA-UPB, 234 av. du Brézet, 63100, Clermont-Ferrand, France

    J. Koenig, S. Bernard & M. Bernard

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  1. E. D. Badaeva
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  2. O. S. Dedkova
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  3. J. Koenig
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  4. S. Bernard
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  5. M. Bernard
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Correspondence to E. D. Badaeva.

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Communicated by M. Kearsey.

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Badaeva, E.D., Dedkova, O.S., Koenig, J. et al. Analysis of introgression of Aegilops ventricosa Tausch. genetic material in a common wheat background using C-banding. Theor Appl Genet 117, 803–811 (2008). https://doi.org/10.1007/s00122-008-0821-4

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