Abstract
Two eyespot resistance genes (Pch1 and Pch2) have been characterised in wheat. The potent resistance gene Pch1, transferred from Aegilops ventricosa, is located on the distal end of the long arm of chromosome 7D (7DL). Pch2 derives from the variety Cappelle Desprez and is located at the distal end of chromosome 7AL. The RFLP marker Xpsr121 and the endopeptidase isozyme allele Ep-D1b, are very closely linked to Pch1, probably due to reduced recombination in the region of the introgressed A. ventricosa segment. Pch2 is less closely linked to these markers but is thought to be closer to Xpsr121 than to Ep-A1b. In the present study simple sequence repeat (SSR) markers were integrated into the genetic map of a single chromosome (7D) recombinant (RVPM) population segregating for Pch1. Sequence-tagged-site (STS)-based assays were developed for Xpsp121 and a 7DL wheat EST containing a SSR. SSR markers Xwmc14 and Xbarc97 and the Xpsr121-derived marker co-segregated with Pch1 in the RVPM population. A single chromosome (7A) recombinant population segregating for Pch2 was screened for eyespot resistance and mapped using SSRs. QTL interval mapping closely associated Pch2 with the SSR marker Xwmc525.
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Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580
Bruehl GW, Nelson WL (1964) Technique for mass inoculations of winter wheat in the field with Cercosporella herpotrichoides. Plant Disease Rep 48:863–865
Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterisation of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563
Chalmers KJ, Campbell AW, Kretschmer J, Karakousis A, Henschke PH, Pierens S, Harker N, Pallotta M, Cornish GB, Shariflou MR, Rampling LR, McLauchlan A, Daggard G, Sharp PJ, Holton TA, Sutherland MW, Appels R, Langridge P (2001) Construction of three linkage maps in bread wheat, Triticum aestivum. Aust J Agr Res 52:1089–1119
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homologous group 7 chromosomes. Theor Appl Genet 78:495–504
Crous PW, Groenewald JZE, Gams W (2003) Eyespot of cereals revisited: ITS phylogeny reveals new species relationships. Eur J Plant Pathol 109:841–850
de la Peña RC, Murray TD, Jones SS (1996) Linkage relations among eyespot resistance gene Pch2, endopeptidase Ep-A1b, and RFLP marker Xpsr121 on chromosome 7A of wheat. Plant Breed 115:273–275
de la Peña RC, Murray TD, Jones SS (1997) Identification of an RFLP interval containing Pch2 on chromosome 7AL in wheat. Genome 40:249–252
Doussinault G, Delibes A, Sanchez-Monge R, Garcia-Olmedo F (1983) Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat. Nature 303:698–700
Fitt BDL (1992) Eyespot of cereals. In: Singh US, Mukhopadhyo AN, Kumar J, Chaube HS (eds) Plant diseases of international importance, diseases of cereals and pulses, vol I. Prentice-Hall, Englewood cliffs, pp 336–354
Groenewald JZ, Marais AS, Marais GF (2003) Amplified fragment length polymorphism-derived microsatellite sequence linked to the Pch1 and Ep-D1 loci in common wheat. Plant Breed 122:83–85
Gupta PK, Rustgi S, Sharma S, Singh R, Kumar N, Balyan HS (2003) Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol Genet Genomics 270:315–323
Hart GE, Langston PJ (1977) Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39:263–277
Hollins TW, Lockley KD, Blackman JA, Scott PR, Bingham J (1988) Field performance of Rendezvous, a wheat cultivar with resistance to eyespot (Pseudocercosporella herpotrichoides) derived from Aegilops ventricosa. Plant Pathol 37:251–260
Johnson R (1984) A critical analysis of durable resistance. Annu Rev Phyto 22:309–330
Johnson R (1992) Past, present and future opportunities in breeding for disease resistance, with examples from wheat. Euphytica 63:3–22
Koebner R, Summers R (2002) The impact of molecular markers on the wheat breeding paradigm. Cell Mol Biol Lett 7:695–702
Koebner RMD, Martin PK (1990) Association of eyespot resistance in wheat cv Cappelle Desprez with endopeptidase profile. Plant Breed 104:312–317
Koebner RMD, Miller TE, Snape JW, Law CN (1988) Wheat endopeptidase––genetic-control, polymorphism, intrachromosomal gene location, and alien variation. Genome 30:186–192
Law CN, Scott PR, Worland AJ, Hollins TW (1976) The inheritance of resistance to eyespot (Cercosporella herpotrichoides) in wheat. Genet Res Camb 25:73–79
Leonard JM, Watson CJW, Carter AH, Hansen JL, Zemetra RS, Santra DK, Campbell KG, Riera-Lizarazu O (2007) Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1. Theor Appl Genet. doi:10.1007/s00122-007-0664-4
Maia N (1967) Obtention de blés tendres résistants au piétin-verse (Cercosporella herpotrichoides) par crôisements interspécifiques blés x Aegilops. C R Acad Agric Fr 53:149–154
McMillin DE, Allan RE, Roberts DE (1986) Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theor Appl Genet 72:743–747
Mena M, Doussinault G, Lopez-Braña I, Aguaded S, García-Olmedo F, Delibes A (1992) Eyespot resistance gene Pch-1 in H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b. Theor Appl Genet 83:1044–1047
Muranty H, Jahier J, Tanguy AM, Worland AJ, Law C (2002) Inheritance of resistance of wheat to eyespot at the adult stage. Plant Breed 121:536–538
Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: Implications for the transferability of SSRs in plants. Mol Biol Evol 15:1275–1287
Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697
Röder MS, Korzun V, Wendehak K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333
Santra DK, Watt C, Little L, Kidwell K, Campbell KG (2006) Comparison of a modified assay method for the endopeptidase marker Ep-D1b with the sequence Tag Site marker XustSSR2001-7DL for strawbreaker foot rot resistance in wheat. Plant Breed 125:13–18
Scott PR (1971) The effect of temperature on eyespot (Cercosporella herpotrichoides) in wheat seedlings. Ann Appl Biol 68:169–175
Scott PR, Hollins TH, Summers RW (1989) Breeding for resistance to two soil-borne diseases of cereals. Vortr Pflanzenzüchtg 16:217–230
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Strausbaugh CA, Murray TD (1989) Inheritance of resistance to Pseudocercosporella herpotrichoides in three cultivars of winter wheat. Phytopathology 79:1048–1053
van Ooijen JW, Maliepaard C (1996) MapQTL (tm) version 4.0: Software for the calculation of QTL positions on genetic maps. CPRO-DLO, Wageningen
Varshney RK, Graner A, Sorrells ME (2005) Genic microsatellite markers in plants: features and applications. Trends Biotech 23:48–55
Worland AJ, Law CN, Hollins TW, Koebner RMD, Giura A (1988) Location of a gene for resistance to eyespot (Pseudocercosporella herpotrichoides) on chromosome 7D of bread wheat. Plant Breed 101:43–51
Acknowledgments
We wish to thank the John Innes Centre Genome Laboratory (JGL) for Pch2 SSR analysis. Thanks to Bill Hollins and members of his team who kindly co-ordinated the second disease trial. This work was jointly funded by BBSRC and Nickerson (UK) Ltd through a BBSRC-CASE award to N. H. Chapman, and BBSRC and the Home Grown Cereal Authority (HGCA) through a BBSRC-CASE award to C. Burt.
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Communicated by A. Kilian.
N. H. Chapman and C. Burt contributed equally to this work.
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Chapman, N.H., Burt, C., Dong, H. et al. The development of PCR-based markers for the selection of eyespot resistance genes Pch1 and Pch2 . Theor Appl Genet 117, 425–433 (2008). https://doi.org/10.1007/s00122-008-0786-3
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DOI: https://doi.org/10.1007/s00122-008-0786-3