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Development of a new diagnostic marker for growth habit selection in faba bean (Vicia faba L.) breeding

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Abstract

Faba bean varieties with determinacy of the apical meristem are relevant to green production. A diagnostic CAPS (cleavage amplification polymorphic sequence) marker for determinate growth habit (ti) in faba bean was previously developed by Avila et al. (Mol Breed 17:185–190, 2006) but was effective only on a limited range of cultivars or genotypes. In this study, we studied the reasons for this limited application and developed a new marker useful for most faba bean-breeding programs. By designing a new set of primers, the complete genomic Vf_TFL1 sequences from different genotypes contrasting for the character were obtained and additional base changes associated with the ti phenotype were identified. The comparison among faba bean sequences showed that the previous CAPS marker was based on a SNP (single nucleotide polymorphism) at position 469 in the intron 2–3, a silent mutation. On the contrary, a SNP at position 26 that distinguishes determinate and indeterminate growth habit genotypes lead to an amino acid change (Leu-9 to Arg) in the determinate growth habit genotypes that could account for the ti phenotype. A dCAPS marker based on this SNP that creates a TaqI site in the ti allele was developed. The marker was 100% successful in predicting ti phenotypes in a broad range of faba bean germplasm representing all major cultivars historically grown in Europe. The outcome confirms the utility of the new dCAPS in worldwide marker-assisted selection programs.

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Acknowledgments

C. M. Avila acknowledges financial support from “Juan de la Cierva” program from MEC (Ministerio de Educación y Ciencia). S.G. Atienza acknowledges financial support from ‘Ramón y Cajal’ program, also from MEC. This research was granted by projects PTR1995-0682-OP from MEC and RTA-04-067 from INIA (Instituto Nacional de Investigaciones Agrarias).

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Authors and Affiliations

  1. Departamento de Genética-ETSIAM, Universidad de Córdoba, Edificio Mendel, Campus de Rabanales, 14071, Córdoba, Spain

    C. M. Avila

  2. Departamento de Mejora Genética Vegetal, IAS-CSIC, Apdo. 4084, 14080, Córdoba, Spain

    S. G. Atienza

  3. Area de Mejora y Biotecnología, IFAPA-Centro Alameda del Obispo, Junta de Andalucía, Apdo. 3092, 14080, Córdoba, Spain

    M. T. Moreno & A. M. Torres

Authors
  1. C. M. Avila
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  2. S. G. Atienza
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  3. M. T. Moreno
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  4. A. M. Torres
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Correspondence to C. M. Avila.

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Communicated by D. Hoisington.

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Avila, C.M., Atienza, S.G., Moreno, M.T. et al. Development of a new diagnostic marker for growth habit selection in faba bean (Vicia faba L.) breeding. Theor Appl Genet 115, 1075–1082 (2007). https://doi.org/10.1007/s00122-007-0633-y

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  • DOI: https://doi.org/10.1007/s00122-007-0633-y

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