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Retrotransposon and gene activation in wheat in response to mycotoxigenic and non-mycotoxigenic-associated Fusarium stress

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Abstract

Despite inhibition of protein synthesis being its mode of action, the trichothecene mycotoxin deoxynivalenol (DON) induced accumulation of transcripts encoding translation elongation factor 1α (EF-1α), class III plant peroxidase (POX), structure specific recognition protein, basic leucine zipper protein transcription factor (bZIP), retrotransposon-like homologs and genes of unknown function in the roots of wheat cultivars CM82036 and Remus. Fusarium head blight (FHB) studies using Fusarium graminearum and its trichothecene-minus (Tri5 −) mutant derivative and adult plant DON tests showed that these transcripts were responsive to both mycotoxigenic- and non-mycotoxigenic-associated Fusarium stress. In tests using the parents ‘CM82036’, ‘Remus’ and 14 double-haploid progeny that segregated for quantitative trait locus (QTL) Fhb1 on chromosome 3BS (syn. Qfhs.ndsu-3BS) (from ‘CM82036’ that confers DON tolerance), bZIP expression was significantly more DON-up-regulated in lines that inherited this QTL. Basal accumulation of the bZIP transcript in spikelets treated with Tween20 (control), DON and in DON-relative to Tween20-treated spikelets was negatively correlated with DON-induced bleaching above (but not below) the treated spikelets (AUDPCDON) (r = −0.41, −0.75 and −0.72, respectively; P ≤ 0.010). bZIP-specific PCR analysis of ‘Chinese spring’ and its 3BS deletion derivatives indicated that bZIP is located in chromosomal region(s) other than 3BS. These results, and the fact that a homologous cold-regulated wheat bZIP (wLIP19) maps to group 1 chromosomes suggests that wheat bZIP may participate in defence response cascades associated with Fhb1 and that there is a cross-talk between biotic and abiotic stress signalling pathways.

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Abbreviations

BZIP:

Basic leucine zipper protein

cv. or cvs:

Cultivar(s)

DDRT-PCR:

Differential display reverse transcriptase-polymerase chain reaction

DON:

Deoxynivalenol

EF-1α:

Translation elongation factor 1 alpha

FHB:

Fusarium head blight

GAPDH:

Glyceraldehyde phosphate dehydrogenase

GDNA:

Genomic DNA

LTR:

Long terminal repeat

PCR:

Polymerase chain reaction

POX:

Peroxidase

QTL:

Quantitative trait loci

RT-PCR:

Reverse transcriptase-PCR

SSRP:

Structure-specific recognition protein

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Acknowledgments

This research was funded by EU FP5 project FUCOMYR (QLRT-2000-02044) and Science Foundation Ireland. We thank Austrian and UK partners (Hermann Buerstmayr, IFA-Tulln, Austria and Paul Nicholson, JIC-UK), and the Wheat Genetics Resource Center of Kansas State University (Manhattan, KS, USA) for providing wheat seed. We thank Robert Proctor (USDA Agricultural Research Service, Peoria, IL, USA) for providing the Fusarium strains.

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Authors and Affiliations

  1. Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Sciences, College of Life Sciences, University College Dublin, Belfield, Dublin 4, Ireland

    Khairul I. Ansari, Stephanie Walter, Josephine M. Brennan, Angela McGahern, Damian Egan & Fiona M. Doohan

  2. Institute for Plant Production Biotechnology, Department IFA-Tulln, BOKU, University of Natural Resources and Applied Life Sciences, Konrad Lorenzstr. 20, 3430, Tulln, Austria

    Marc Lemmens

  3. Department of Botany and Plant Pathology, Lilly Hall, Purdue University, 915 West State Street, West Lafayette, IN, 47907-2054, USA

    Sarah Kessans

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  1. Khairul I. Ansari
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  2. Stephanie Walter
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Correspondence to Khairul I. Ansari.

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Communicated by D. A. Hoisington.

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Ansari, K.I., Walter, S., Brennan, J.M. et al. Retrotransposon and gene activation in wheat in response to mycotoxigenic and non-mycotoxigenic-associated Fusarium stress. Theor Appl Genet 114, 927–937 (2007). https://doi.org/10.1007/s00122-006-0490-0

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