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A genetic linkage map for tef [Eragrostis tef (Zucc.) Trotter]

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Abstract

Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in Ethiopia. Tef is an allotetraploid with a base chromosome number of 10 (2n = 4x = 40) and a genome size of 730 Mbp. Ninety-four F9 recombinant inbred lines (RIL) derived from the interspecific cross, Eragrostis tef cv. Kaye Murri × Eragrostis pilosa (accession 30-5), were mapped using restriction fragment length polymorphisms (RFLP), simple sequence repeats derived from expressed sequence tags (EST–SSR), single nucleotide polymorphism/insertion and deletion (SNP/INDEL), intron fragment length polymorphism (IFLP) and inter-simple sequence repeat amplification (ISSR). A total of 156 loci from 121 markers was grouped into 21 linkage groups at LOD 4, and the map covered 2,081.5 cM with a mean density of 12.3 cM per locus. Three putative homoeologous groups were identified based on multi-locus markers. Sixteen percent of the loci deviated from normal segregation with a predominance of E. tef alleles, and a majority of the distorted loci were clustered on three linkage groups. This map will be useful for further genetic studies in tef including mapping of loci controlling quantitative traits (QTL), and comparative analysis with other cereal crops.

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Acknowledgments

J.-K. Yu and R.V. Kantety contributed equally to the research described herein. The authors would like to acknowledge financial support from the McKnight Foundation Collaborative Crop Research Program (Grant # 00-933) and Hatch project 149419. We are grateful to Drs. Susan McCouch at Cornell University, Bikram Gill at Kansas State University, Andrew Paterson and Katrien Devos at University of Georgia for providing the primers of RM, KSUM, SRSC/PRSC and inf/lfm markers, respectively. We also wish to acknowledge R. Greene, C. Bermudez and C. T. Jayasuriya for their generous assistance.

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Author notes

  1. Ramesh V. Kantety

    Present address: Department of Plant and Soil Science, Alabama A&M University, 134 ARC Building, P.O. Box 1927, Normal, AL, 35762, USA

Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Cornell University, 240 Emerson Hall, Ithaca, NY, 14853, USA

    Ju-Kyung Yu, Ramesh V. Kantety, Elizabeth Graznak, David Benscher & Mark E. Sorrells

  2. Debre Zeit Agricultural Research Center, P.O. Box 32, Debre Zeit, Ethiopia

    Hailu Tefera

Authors
  1. Ju-Kyung Yu
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  2. Ramesh V. Kantety
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  3. Elizabeth Graznak
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  4. David Benscher
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  5. Hailu Tefera
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  6. Mark E. Sorrells
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Correspondence to Mark E. Sorrells.

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Communicated by C. Gebhardt.

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Yu, JK., Kantety, R.V., Graznak, E. et al. A genetic linkage map for tef [Eragrostis tef (Zucc.) Trotter]. Theor Appl Genet 113, 1093–1102 (2006). https://doi.org/10.1007/s00122-006-0369-0

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  • DOI: https://doi.org/10.1007/s00122-006-0369-0

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