Abstract
The restoration of the C-type cytoplasmic male sterility (Cms) has been a common agriculture practice in the production of hybrid seed for many years. In this study, a series of crosses between select sterile and restorer lines, as well as a backcross population of (Cms-C77 × 6233) × 6233, were used to investigate the restoration of C-type Cms. Our results demonstrated that there was an inhibitor of the Rf5 restorer gene. This inhibitor gene, Rf-I, maps to chromosome 7 and is tightly linked with SSR markers, umc2326 and umc2327, at a genetic distance 4.7 and 3.4 cM, respectively. After analyzing our data combined with previous studies, we propose that the restoration of C-type Cms has two dominant genes, Rf4 and Rf5. Rf4 has the ability to restore all genotypes of Cms-C lines; however, there exists an inhibitor for the other restorer gene, Rf5; thus, it can restore only those genotypes of Cms-C lines lacking the Rf-I inhibitor.
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References
Beckett JB (1971) Classification of male sterile cytoplasms in maize (Zea mays L.). Crop Sci 11:724–726
Chen WC, Luo FH, Ji LY (1979) Some genetic aspects of the C-type cytoplasmic male-sterility in maize and its use in breeding. Acta Agronom Sin 5(4):21–28
Duvick DN (1972) Potential usefulness of new cytoplasmic male sterile and sterility system. In: Proceedings of the 27th annual corn and sorghum research conference, pp 197–201
Duvick DN, Snyder RJ, Anderson EC (1961) The chromosomal location of Rf1, a restorer gene for cytoplasmic pollen sterile maize. Genetics 46:1245–1252
Josephson LM, Morgan TE (1978) Genetics and inheritance of fertility restoration of male sterile cytoplasms in corn. In: Proceedings of the 33rd corn and sorghum research conference 7:13
Khey-Pour A, Grace VE, Everett HL (1981) Genetics of fertility restorations in the C-group of cytoplasmic male sterility in maize. Genetics 98:379–388
Li JX, Dai JR, Xu QF (1963) Study on the inheritance of cytoplasmic male-sterile and fertility restoration in maize. Acta Agronom Sin 2:339–362
Michelmore RW, Paran I, Kesseli V (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic region is by using segregating population. Pro Natl Aca Sci USA 88:9828–9832
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Qin TC, Xu ML, Deng DX (1990) Cytoplasmic male-sterility: identification of the number of restorer genes. Maize Genet Coop News Lett 64:124
Qin TC, Xu ML, Deng DX, Chen RL, Bian YL (2001) Study on male-sterility in Zea mays VIII. RFLP analysis of mtDNA of YII-1 type maize male-sterile cytoplasm. Acta Agronom Sin 27:185–189
Sisco PH (1991) Duplication complicates genetic mapping of Rf4 a restoring gene for cms-C cytoplasmic male sterility in corn. Crop Sci 31:1263–1266
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744
Tang JH, Ji HQ, Huang ZW, Ji LY, Wang CH (2001a) Mapping of the main restorer gene (Rf4) of C-cytoplasmic male sterility in maize with RFLP. J Henan Agric Univ 35:99–102
Tang JH, Liu ZH, Chen WC, Hu YM, Ji LY (2001b) Mapping the main restorer genes of C-cytoplasmic male sterility using SSR method. Acta Agronom Sin 34:592–596
Vidakovic M (1988) Genetics of fertility restoration in cytoplasmic male sterility of the C-type (Cms-c) in maize. Maydica 33:51–64
Vidakovic M, Vancetovic J, Vidakovic M (1997a) Complementary genes Rf4, Rf5 and Rf6 are not the unique genetic system for fertility restoration in cmsC of maize (Zea mays L.). Maize Genet Coop News Lett 71:10
Vidakovic M, Vancetovic J, Vidakovic M (1997b) The existence of a duplicated or parallel genetic system for fertility restoration in cmsC of maize (Zea mays L.). Maydica 42:313–316
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This work was supported by the National High-technology Project of China and National Natural Science Foundation of China.
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Hu, Y.M., Tang, J.H., Yang, H. et al. Identification and mapping of Rf-I an inhibitor of the Rf5 restorer gene for Cms-C in maize (Zea mays L.). Theor Appl Genet 113, 357–360 (2006). https://doi.org/10.1007/s00122-006-0302-6
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DOI: https://doi.org/10.1007/s00122-006-0302-6