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Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions

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Abstract

Despite the collective efforts of the international community to sequence the complete rice genome, telomeric regions of most chromosome arms remain uncharacterized. In this report we present sequence data from subtelomere regions obtained by analyzing telomeric clones from two 8.8 × genome equivalent 10-kb libraries derived from partial restriction digestion with HaeIII or Sau3AI (OSJNPb HaeIII and OSJNPc Sau3AI). Seven telomere clones were identified and contain 25–100 copies of the telomere repeat (CCCTAAA)n on one end and unique sequences on the opposite end. Polymorphic sequence-tagged site markers from five clones and one additional PCR product were genetically mapped on the ends of chromosome arms 2S, 5L, 10S, 10L, 7L, and 7S. We found distinct chromosome-specific telomere-associated tandem repeats (TATR) on chromosome 7 (TATR7) and on the short arm of chromosome 10 (TATR10s) that showed no significant homology to any International Rice Genome Sequencing Project (IRGSP) genomic sequence. The TATR7, a degenerate tandem repeat which is interrupted by transposable elements, appeared on both ends of chromosome 7. The TATR10s was found to contain an inverted array of three tandem repeats displaying an interesting secondary folding pattern that resembles a telomere loop (t-loop) and which may be involved in a protective function against chromosomal end degradation.

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Acknowledgements

This work was supported by grants from the United States Department of Agriculture Cooperative Research, Education and Extension Service, the National Science Foundation and the Department of Energy (Principal Investigator—RAW).

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Authors and Affiliations

  1. Brassica Genomics Team, National Institute of Agricultural Biotechnology, RDA, Suwon, 441-707, Korea

    Tae-Jin Yang

  2. Arizona Genomics Institute, University of Arizona, 303 Forbes building, Tucson, AZ, 85721, USA

    Tae-Jin Yang, Yeisoo Yu & Rod A. Wing

  3. Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands

    Song-Bin Chang & Hans de Jong

  4. Department of Agronomy, ChungNam Nat’l University, Daejeon, Korea

    Chang-Sik Oh & Sang-Nag Ahn

  5. Clemson University, Clemson, SC, 29760, USA

    Eric Fang

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  1. Tae-Jin Yang
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Correspondence to Rod A. Wing.

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Yang, TJ., Yu, Y., Chang, SB. et al. Toward closing rice telomere gaps: mapping and sequence characterization of rice subtelomere regions. Theor Appl Genet 111, 467–478 (2005). https://doi.org/10.1007/s00122-005-2034-4

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