Skip to main content
SpringerLink
Log in

QTLs mapping for fruit size and shape in chromosomes 2 and 4 in pepper and a comparison of the pepper QTL map with that of tomato

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Quantitative trait locus (QTL) mapping for fruit weight and shape in pepper (Capsicum spp.) was performed using C. chinense and C. frutescens introgression lines of chromosomes 2 and 4. In chromosome 2, a single major fruit-weight QTL, fw2.1, was detected in both populations that explained 62% of the trait variation. This QTL, as well as a fruit-shape QTL, fs2.1, which had a more minor effect, were localized to the tomato fruit-shape gene ovate. The cloned tomato fruit-weight QTL, fw2.2, did not play a major role in controlling fruit size variations in pepper. In chromosome 4, two fruit-weight QTLs, fw4.1 and fw4.2, were detected in the same genomic regions in both mapping populations. In addition, a single fruit-shape QTL was detected in each of the mapping populations that co-localized with one of the fruit-weight QTLs, suggesting pleiotropy or close linkage of the genes controlling size and shape. fw2.1 and fw4.2 represent major fruit-weight QTLs that are conserved in the three Capsicum species analyzed to date for fruit-size variations. Co-localization of the pepper QTLs with QTLs identified for similar traits in tomato suggests that the pepper and tomato QTLs are orthologous. Compared to fruit-shape QTLs, fruit-weight QTLs were more often conserved between pepper and tomato. This implies that different modes of selection were employed for these traits during domestication of the two Solanaceae species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Ben Chaim A (2002) Analysis of quantitative traits in pepper (Capsicum spp.) using molecular markers and comparative mapping with tomato. A PhD thesis submitted to the Hebrew University of Jerusalem

  • Ben Chaim A, Paran I, Grube R, Jahn M, van Wijk R, Peleman J (2001) QTL mapping of fruit related traits in pepper (Capsicum annuum). Theor Appl Genet 102:1016–1028

    Article  Google Scholar 

  • Ben Chaim A, Borovsky E, Rao GU, Tanyolac B, Paran I (2003a) fs3.1: a major fruit shape QTL conserved in Capsicum. Genome 46:1–9

    Article  CAS  Google Scholar 

  • Ben Chaim A, Borovsky Y, De Jong W, Paran I (2003b) Linkage of the A locus for the presence of anthocyanin and fs10.1, a major fruit-shape QTL in pepper. Theor Appl Genet 106:889–894

    Google Scholar 

  • Cong B, Liu J, Tanksley SD (2002) Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations. Proc Natl Acad Sci USA 99:13606–13611

    Article  CAS  PubMed  Google Scholar 

  • Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002a) A comparative genetic linkage map of eggplant (Solanum melongena) and its implications for genome evolution in the Solanaceae. Genetics 161:1697–1711

    CAS  Google Scholar 

  • Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002b) Conservation of gene function in the Solanaceae as revealed by comparative mapping of domestication traits in eggplant. Genetics 161:1713–1726

    CAS  Google Scholar 

  • Eshed Y, Zamir D (1995) An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141:1147–1162

    CAS  PubMed  Google Scholar 

  • Frary A, Nesbitt TC, Frary A, Grandillo S, van der Knapp E, Cong B, Liu J, Meller J, Elber R, Alpert KB, Tanksley SD (2000) fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289:85–88

    Article  CAS  PubMed  Google Scholar 

  • Fridman E, Liu YS, Carmel-Goren L, Gur A, Shoresh M, Pleban T, Eshed Y, Zamir D (2002) Two tightly linked QTLs modify tomato sugar content via different physiological pathways. Mol Genet Genomics 266:821–826

    Article  CAS  PubMed  Google Scholar 

  • Grandillo S, Ku HM, Tanksley SD (1999) Identifying the loci responsible for natural variation in fruit size and shape in tomato. Theor Appl Genet 99:978–987

    Article  CAS  Google Scholar 

  • Grube RC, Radwanski ER, Jahn MM (2000) Comparative genetics of disease resistance within the Solanaceae. Genetics 155:873–887

    CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Lecomte L, Saliba-Colombani V, Gautier A, Gomez-Jimenez MC, Duffé P, Buret M, Causse M (2004) Fine mapping of QTLs of chromosome 2 affecting the fruit architecture and composition of tomato. Mol Breed 13:1–14

    Article  CAS  Google Scholar 

  • Lippman Z, Tanksley SD (2001) Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L. esculentum, var. Giant Heirloom. Genetics 158:413–422

    CAS  PubMed  Google Scholar 

  • Liu J, Van Eck J, Cong B, Tanksley SD (2002) A new class of regulatory genes underlying the cause of pear-shaped tomato fruit. Proc Natl Acad Sci USA 99:13302–13306

    Google Scholar 

  • Livingstone KD, Lackney VK, Blauth J, Wijk VR, Jahn MK (1999) Genome mapping in Capsicum and the evolution of genome structure in the Solanaceae. Genetics 152:1183–1202

    CAS  PubMed  Google Scholar 

  • Monforte A, Friedman E, Zamir D, Tanksley SD (2001) Comparison of a set of alleleic QTL-NILs for chromosome 4 of tomato: deductions about natural variation and implications for germplasm collection. Theor Appl Genet 102:572–590

    Article  CAS  Google Scholar 

  • Nelson CJ (1997) qgene: software for marker-based genomic analysis and breeding. Mol Breed 3:229–235

    Article  Google Scholar 

  • Paterson AH, Bowers JE, Burow MD, Draye X, Elsik CG, Jiang CX, Katsar CS, Lan TH, Lin YR, Ming R, Wright RJ (2000) Comparative genomics of plant chromosomes. Plant Cell 12:1523–1539

    Article  CAS  PubMed  Google Scholar 

  • Rao GU, Ben Chaim A, Borovsky E, Paran I (2003) Mapping of yield related QTLs in pepper in an inter-specific cross of Capsicum annuum and C. frutescens. Theor Appl Genet 106:1457–1466

    CAS  PubMed  Google Scholar 

  • SAS Institute (2000) JMP Users Guide: version 4.0. SAS Institute, Cary, N.C.

    Google Scholar 

  • Tanksley SD (2004) The genetic, developmental, and molecular bases of fruit size and shape variation in tomato. Plant Cell 16:S181–S189

    Article  CAS  PubMed  Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, deVicente MC, Bonierbale MW, Broun P, Fulton TM, Giovanonni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Thorup TA, Tanyolac B, Livingstone KD, Popovsky S, Paran I, Jahn M (2000) Candidate gene analysis of organ pigmentation loci in the Solanaceae. Proc Natl Acad Sci USA 97:11192–11197

    Google Scholar 

  • Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C (2002) mapqtl 4.0, software for the calculation of QTL positions on genetic maps. Plant research international, Wageningen

    Google Scholar 

  • Wilson LM, Whitt SR, Ibanez AM, Rocheford TR, Goodman MM, Buckler IV ES (2004) Dissection of maize kernel composition and starch production by candidate gene association. Plant Cell 16:2719–2733

    Article  CAS  PubMed  Google Scholar 

  • Yates HE, Frary A, Doganla S, Frampton A, Eannetta NT, Uhlig J, Tanksley SD (2004) Comparative fine mapping of fruit quality QTLs on chromosome 4 introgressions derived from two wild tomato species. Euphytica 135:283–296

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by The Israel Science Foundation (Grant No. 643/00) and by The United States-Israel Binational Agricultural Research and Development Fund Grant No. IS-3225-01C.

Author information

Authors and Affiliations

  1. Department of Plant Genetics and Breeding, The Volcani Center, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel

    S. Zygier, A. B. Chaim, A. Efrati, G. Kaluzky, Y. Borovsky & I. Paran

Authors
  1. S. Zygier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. B. Chaim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Efrati
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Kaluzky
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Borovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. Paran
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. Paran.

Additional information

Communicated by R. Hagemann

S. Zygier and A. Ben Chaim contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zygier, S., Chaim, A.B., Efrati, A. et al. QTLs mapping for fruit size and shape in chromosomes 2 and 4 in pepper and a comparison of the pepper QTL map with that of tomato. Theor Appl Genet 111, 437–445 (2005). https://doi.org/10.1007/s00122-005-2015-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-2015-7

Keywords

Search

Navigation