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Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.).

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Abstract

A microsatellite-based high-density linkage map for oil palm (Elaeis guinensis Jacq.) was constructed from a cross between two heterozygous parents, a tenera palm from the La Mé population (LM2T) and a dura palm from the Deli population (DA10D). A set of 390 simple sequence repeat (SSR) markers was developed in oil palm from microsatellite-enriched libraries and evaluated for polymorphism along with 21 coconut SSRs. A dense and genome-wide microsatellite framework as well as saturating amplified fragments length polymorphisms (AFLPs) allowed the construction of a linkage map consisting of 255 microsatellites, 688 AFLPs and the locus of the Sh gene, which controls the presence or absence of a shell in the oil palm fruit. An AFLP marker E-Agg/M-CAA132 was mapped at 4.7 cM from the Sh locus. The 944 genetic markers were distributed on 16 linkage groups (LGs) and covered 1,743 cM. Our linkage map is the first in oil palm to have 16 independent linkage groups corresponding to the plant’s 16 homologous chromosome pairs. It is also the only high-density linkage map with as many microsatellite markers in an Arecaceae species and represents an important step towards quantitative trait loci analysis and physical mapping in the E. guineensis species.

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Acknowledgements

Our sincere thanks are extended to Genoscope (Evry, France) for sequencing the oil palm microsatellite clones. Likewise, we thank the Commission of the European Communities for its financial backing of this research (EC project no. ICA4-CT-2001-10066—Directorate General of Research—INCO-Dev.). We are grateful to the SOCFINDO estate (Medan, Indonesia) and CNRA La Mé Station (Ivory Coast) for providing plant samples and phenotypic observations for this study. We would also like to thank Dr. T. Schiex (INRA, France) for his help in using the carthagene software. We are grateful to Dr. M. Seguin (Cirad, France) for his advice on the statistics. Finally, we would like to thank two anonymous reviewers for their comments and fruitful suggestions for the improvement of this publication.

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Authors and Affiliations

  1. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1096 Polymorphismes d’Intérêt Agronomique, TA 40/03 Avenue Agropolis, 34398, Montpellier Cedex 5, France

    N. Billotte, N. Marseillac, A.-M. Risterucci, F.-C. Baurens, C. Billot, P. Amblard, T. Durand-Gasselin & B. Courtois

  2. Centre National de Recherche Agronomique (CNRA), Station de La Mé, 13, BP 989, Abidjan, 13, Ivory coast

    B. Adon

  3. ENSAM, INRA, Université Montpellier II, 2 Place Pierre Viala, 34060, Montpellier Cedex 01, France

    A. Charrier

  4. Centre National de Séquençage (GENOSCOPE), 2 rue Gaston Crémieux, CP 5706, 91057, Evry Cedex, France

    P. Brottier

  5. Indonesian Oil Palm Research Institute (IOPRI), Jl. Brigjen Katamso 51, Medan, 20158, Indonesia

    D. Asmono

  6. Centro de Arkaute, Departamento de Producción y Proteccion Vegetal, NEIKER, Apartado 46, 01080, Vitoria, Spain

    A. Herrán & E. Ritter

  7. Max-Planck-Institut für Zuechtungsforschung (MPIZ), Carl-von-Linne-Weg 10, 50829, Cologne, Germany

    W. Rohde

  8. Malaysia Palm Oil Board (MPOB), 6 Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang Selangor, Malaysia

    R. Singh & S. C. Cheah

  9. SOCFINDO (P.T. Socfin Indonesia), Jl. K.L. Yos Sudarso No. 105 Medan 20115, P.O. Box 1254, Medan, 2001, Indonesia

    H. Asmady

Authors
  1. N. Billotte
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  2. N. Marseillac
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  3. A.-M. Risterucci
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  4. B. Adon
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  5. P. Brottier
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  6. F.-C. Baurens
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  7. R. Singh
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  8. A. Herrán
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  9. H. Asmady
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  10. C. Billot
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  11. P. Amblard
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  12. T. Durand-Gasselin
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  13. B. Courtois
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  14. D. Asmono
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  15. S. C. Cheah
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  16. W. Rohde
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  17. E. Ritter
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  18. A. Charrier
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Corresponding author

Correspondence to N. Billotte.

Additional information

Communicated by O. Savolainen

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Billotte, N., Marseillac, N., Risterucci, AM. et al. Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.).. Theor Appl Genet 110, 754–765 (2005). https://doi.org/10.1007/s00122-004-1901-8

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  • DOI: https://doi.org/10.1007/s00122-004-1901-8

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