Abstract
The stem rust resistance gene Sr2 has provided durable broad-spectrum, adult-plant resistance to the fungal pathogen Puccinia graminis Pers. f. sp. tritici throughout wheat-growing regions of the world for more than 50 years. The ability to select for Sr2 in wheat breeding programs was recently improved by the identification of a tightly linked microsatellite marker gwm533. This marker typically amplifies a 120-bp polymerase chain reaction fragment from wheat lines carrying Sr2. In instances where the 120-bp fragment is not associated with the presence of Sr2, DNA sequence analysis has shown that a second allele was amplified, differing in the structure of the microsatellite repeat. To discriminate this allelic homoplasy (alleles identical in size, but not identical by descent), sequence-tagged microsatellites (STM) markers were developed for the Xgwm533 locus. These markers were shown to be diagnostic for the presence of Sr2 in a wide range of germplasm, representative of all major wheat varieties historically grown in Australia. The STMs will be particularly useful for marker-assisted selection in Southern Australian breeding programs, where the use of the marker gwm533 is often precluded by the presence of the non-Sr2-associated 120-bp allele in the pedigree of current breeding germplasm. The STMs also revealed a high incidence of previously undetected allelic homoplasy at the Xgwm533 locus and may have broader utility in genetic research and breeding, as this locus is also reported to be strongly associated with a major gene conferring resistance to Fusarium head blight.
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Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Mitchell Fetch J, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor Appl Genet 102:1164–1168
Bariana HS, Bell JA, McIntosh RA (1998) Cereal cultivars, rust reactions and other information. National Cereal Rust Control Program Circular No. 38, University of Sydney
Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust J Agric Res 52:1247–1255
Benham JJ (2001) Genographer, version 1.6.0. http://hordeum.oscs.montana.edu/genographer
Bhowal JG, Narkhede A (1981) Genetics of pseudo-black chaff in wheat. Z Planzenzuecht 86:298–304
Brown GN (1993) A seedling marker for gene Sr2 in wheat. In: Imrie BC, Hacker JB (eds) Proceedings of the tenth Australian plant breeding conference, vol. 2. Conference Organising Committee, Gold Coast, pp 139–140
Brown GN (1997) The inheritance and expression of leaf chlorosis associated with gene Sr2 for adult plant resistance to wheat stem rust. Euphytica 95:67–71
Buerstmayr H, Steiner B, Hatrl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schnieder B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508
Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ, Henschke PH, Carter M (2001) Implementation of markers in Australian wheat breeding. Aust J Agric Res 52:1349–1356
Grimaldi MC, Crouau-Roy B (1997) Microsatellite allelic homoplasy due to variable flanking sequences. J Mol Evol 44:336–340
Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable, adult-plant resistances in Hope and related cultivars to rusts. Z Planzenzuecht 83:350–367
Hayden MJ, Stephenson P, Logojan AM, Khatkar D, Rogers C, Koebner RMD, Snape JW, Sharp PJ (2004) A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs. Theor Appl Genet 108:733–742
Kammholz SJ, Campbell AW, Sutherland MW, Hollamby GJ, Martin PJ, Eastwood RF, Barclay I, Wilson RE, Brennan PS, Sheppard JA (2001) Establishment and characterisation of wheat genetic mapping populations. Aust J Agric Res 52:1079–1088
McIntosh RA (1988) The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 1–9
McIntosh RA (1992) Pre-emptive breeding to control wheat rusts. Euphytica 63:103–113
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO, Australia
Paull JG, Chalmers KJ, Karakousis A, Kretschmer JM, Manning S, Langridge P (1998) Genetic diversity in Australian wheat varieties and breeding material based on RFLP data. Theor Appl Genet 96:435–446
Rajaram S, Ceccarelli S (1988) International collaboration in cereal breeding. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico pp 533–537
Rajaram S, Singh RP, Torres E (1988) Current CIMMYT approaches in breeding for rust resistance. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 101–118
Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 10–22
Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterisation of wheat–rye recombinants involving chromosome arm 1DS of wheat. Theor Appl Genet 82: 537–554
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336
Sunderwirth SD, Roelfs AP (1980) Greenhouse evaluation of the adult plant resistance of Sr2 to wheat stem rust. Phytopathology 70:634–637
Zhou WC, Kolb FL, Bai GH, Domier LL, Boze LK, Smith NJ (2003) Validation of a major QTL for scab resistance with SSR markers and use of marker assisted selection in wheat. Plant Breeding 122:40–46
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Communicated by J.W. Snape
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Hayden, M.J., Kuchel, H. & Chalmers, K.J. Sequence tagged microsatellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.). Theor Appl Genet 109, 1641–1647 (2004). https://doi.org/10.1007/s00122-004-1787-5
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DOI: https://doi.org/10.1007/s00122-004-1787-5