Skip to main content
SpringerLink
Log in

Molecular genetic mapping of Gby, a new greenbug resistance gene in bread wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The greenbug, Schizaphis graminum (Rhodani), is one of the major insect pests of wheat worldwide and it is important to develop a basic understanding of the chromosomal locations of known and new greenbug resistance genes. Gby is a new greenbug resistance gene in the wheat line ‘Sando’s selection 4040’. A mapping population used in this study was derived from a cross of Sando’s 4040 and PI220127, a greenbug susceptible wheat land race from Afghanistan. A progeny test indicated that Gby is inherited as a single semi-dominant gene. A genetic linkage map consisting of Gby, Xgwm322 (a wheat microsatellite marker), XksuD2 (an STS marker) and 18 restriction fragment length polymorphism (RFLP) loci was constructed. We used DNA from Chinese Spring 7A deletion lines to show that the gwm332 and ksuD2 amplified fragments mapped in this study are located on a long arm of chromosome 7A. This suggests that Gby is located on wheat chromosome 7A. Gby was mapped to the area in the middle of the ‘island’ of putative defense response genes that are represented by RFLP markers (Xpsr119, XZnfp, Xbcd98 and Pr1b) previously mapped to the distal part of the short arm of wheat chromosome group 7. This region of chromosome 7A is characterized by a high recombination rate and a high physical density of markers which makes Gby a very good candidate for map-based cloning. The selection accuracy when the RFLP markers Xbcd98, Xpsr119 or XZnfp and Pr1b flanking Gby are used together to tag Gby is 99.78%, suggesting that they can be successfully used in marker assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–c
Fig. 2

Similar content being viewed by others

References

  • Anonymous (1996) Agricultural statistics (1995–1996) Wheat, National Agricultural Statistics Service, US Department of Agriculture, Washington, p 1

  • Boyko EV, Gill KS, Mickelson-Young L, Nasuda S, Raupp WJ, Ziegle JN, Singh S, Hassawi DS, Fritz AK, Namuth D, Lapitan NLV, Gill BS (1999) A high-density genetic linkage map of Aegilops tauschii, the D-genome progenitor of bread wheat. Theor Appl Genet 99:16–26

    Article  CAS  Google Scholar 

  • Boyko EV, Kalendar R, Korzun V, Fellers J, Korol A, Schulman A, Gill BS (2002) A high density genetic map of Aegilops tauschii incorporating retrotransposons and defense-related genes: insights into cereal chromosome structure and function. Plant Mol Biol 48:767–790

    Article  CAS  PubMed  Google Scholar 

  • Curtis BC, Schlehuber AM, Wood EA Jr (1960) Genetics of greenbug [Toxoptera graminum (Rond.)] resistance in two strains of common wheat. Agron J 52:599–602

    Google Scholar 

  • Dubcovsky J, Lukaszewski AJ, Echaide M, Antonelli EF, Porter DR (1998) Molecular characterization of two Triticum speltoides interstitial translocations carrying leaf rust and greenbug resistance genes. Crop Sci 38:1655–1660

    CAS  Google Scholar 

  • Eddleman BR, Chang CC, McCarl BA (1999) Economic benefits from grain sorghum variety improvement in the United States. In: Wiseman BR, Webster JA (eds) Economic, environmental, and social benefits of resistance in field crops. Entomological Society of America, Thomas Say, Lanham, pp 17–44

  • Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991) Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germplasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389

    Google Scholar 

  • Friebe B, Mukai Y, Gill BS (1992) C-banding polymorphism in several accessions of Triticum tauschii. Genome 35:192–199

    Google Scholar 

  • Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat. Genome 34:362–374

    Google Scholar 

  • Harvey TL, Martin TJ (1990) Resistance to Russian wheat aphid Diuraphis noxia in wheat (Triticum aestivum). Cereal Res Commun 18:127–129

    Google Scholar 

  • Hohmann U, Endo TR, Gill KS, Gill BS (1994) Comparison of genetic and physical maps of group-7 chromosomes from Triticum aestivum L. Mol Gen Genet 245:644–653

    CAS  PubMed  Google Scholar 

  • Hohmann U, Graner A, Endo TR, Gill BS, Herrmann RG (1995) Comparison of wheat physical maps with barley linkage maps for group 7 chromosomes. Theor Appl Genet 91:618–626

    CAS  Google Scholar 

  • Hollenhorst MM, Joppa LR (1983) Chromosomal location of genes for resistance to greenbug in ‘Largo’ and ‘Amigo’ wheats. Crop Sci 23:91–93

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lazar MD, Worrall WD, Peterson GL, Porter KB, Rooney LW, Tuleen NA, Marshall DS, McDaniel ME, Nelson R (1997) Registration of ‘TAM 110’ wheat. Crop Sci 37:1978–1979

    Google Scholar 

  • LeClerg EL, Cook HT, Van Hauweling CD, Anderson RJ, Vance AM, Dorwood KH, Thomas R (1965) Injurious crop insects, losses in agriculture. US Department of Agriculture, Agricultural Research Service, Agriculture Handbook No. 291, Washington, p 43

  • Maestra B, Naranjo T (1998) Homologous relationships of Aegilops speltoides chromosomes to bread wheat. Theor Appl Genet 97:181–186

    Article  Google Scholar 

  • McClave JT, Dietrich FH II (1982) Statistics. Dellen, San Francisco, pp 96, 496–527, 729–730

  • Plaschke J, Börner A, Wendehake K, Ganal MW, Röder MS (1996) The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40

    CAS  Google Scholar 

  • Porter KB, Peterson GL, Vise O (1982) A new greenbug biotype. Crop Sci 22:847–850

    Google Scholar 

  • Porter DR, Webster JA, Burton RL, Puterka GL, Smith EL (1991) New sources of resistance to greenbug in wheat. Crop Sci 31:1502–1504

    Google Scholar 

  • Porter DR, Webster JA, Friebe B (1994) Inheritance of greenbug biotype G resistance in wheat. Crop Sci 34:625–628

    Google Scholar 

  • Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of the wheat genome. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Sambrook J, Maniatis T, Fritsch EF (1998) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Sebesta EE, Wood EA Jr (1978) Transfer of greenbug resistance from rye to wheat with X-rays. Agron Abstr 61–62

  • Smith CM (1999) Plant resistance to insects. In: Rechcigl J, Rechcigl N (eds) Biological and biotechnological control of insects. Lewis, Boca Raton, pp 171–207

  • Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794

    CAS  Google Scholar 

  • Tyler JM, Webster JA, Smith EL (1985) Biotype E greenbug resistance in wheat streak mosaic virus-resistant wheat germplasm lines. Crop Sci 25:686–688

    Google Scholar 

  • Tyler JM, Webster JA, Merkle OG (1987) Designations for genes in wheat germplasm conferring greenbug resistance. Crop Sci 27:526–527

    Google Scholar 

  • Webster JA, Kenkel P (1999) Economic, environmental, and social benefits of resistance in field crops. Entomological Society of America, Thomas Say, Lanham

  • Weng Y, Lazar MD (2002) Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat. Plant Breed 121:218–223

    Article  CAS  Google Scholar 

  • Yencho GC, Cohen MB, Byrne PF (2000) Applications of tagging and mapping insect resistance loci in plants. Annu Rev Entomol 45:393–422

    Article  CAS  PubMed  Google Scholar 

  • Zhu L, Smith CM, Fritz A, Boyko EV, Flinn M (2004) Genetic analysis and molecular mapping of a wheat gene conferring tolerance to the greenbug (Schizaphis graminum Rodani). Theor Appl Genet 104: (in press)

Download references

Acknowledgements

The authors thank Ming Chen and Mitch Tuinstra for reviews of the original manuscript, and Bikram Gill (Kansas State University Wheat Genetic Resource Center) for providing seeds of parents and F2 mapping population. This research was supported by the National Science Foundation, the Kansas Wheat Commission, The Kansas State University Wheat Research Center, and the Kansas Crop Improvement Association. Kansas Agricultural Experiment Station Contribution No. 03-148-5.

Author information

Authors and Affiliations

  1. Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA

    E. Boyko, S. Starkey & M. Smith

Authors
  1. E. Boyko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Starkey
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Boyko.

Additional information

Communicated by J. Dvorak

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boyko, E., Starkey, S. & Smith, M. Molecular genetic mapping of Gby, a new greenbug resistance gene in bread wheat. Theor Appl Genet 109, 1230–1236 (2004). https://doi.org/10.1007/s00122-004-1729-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1729-2

Keywords

Search

Navigation