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Comparative mapping of ZYMV resistances in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.)

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Abstract

Zucchini yellow mosaic virus (ZYMV) routinely causes significant losses in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). ZYMV resistances from the cucumber population ‘TMG1’ and the melon plant introduction (PI) 414723 show different modes of inheritance and their genetic relationships are unknown. We used molecular markers tightly linked to ZYMV resistances from cucumber and melon for comparative mapping. A 5-kb genomic region (YCZ-5) cosegregating with the zym locus of cucumber was cloned and sequenced to reveal single nucleotide polymorphisms and indels distinguishing alleles from ZYMV-resistant (TMG1) and susceptible (Straight 8) cucumbers. A low-copy region of the YCZ-5 clone was hybridized to bacterial artificial chromosome (BAC) clones of melon and a 180-kb contig assembled. One end of this melon contig was mapped in cucumber and cosegregated with ZYMV resistance, demonstrating that physically linked regions in melon show genetic linkage in cucumber. However the YCZ-5 region segregated independently of ZYMV resistance loci in two melon families. These results establish that these sources of ZYMV resistances from cucumber TMG1 and melon PI414723 are likely non-syntenic.

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Acknowledgements

We gratefully acknowledge the gift of F4 melon lines from Vedrantais crossed with PI414723 from Dr. Michel Pitrat, INRA, to Seminis Seed Company and the funding support of Y.H. Park by the Pickle Seed Research Foundation.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA

    Y. Park

  2. Department of Vegetable Crops, Agricultural Research Organization, Newe Ya’ar Research Center, PO Box 1021, Ramat Yishay, 30095, Israel

    N. Katzir & Y. Brotman

  3. Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 52900, Israel

    Y. Brotman

  4. Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA 95695, USA

    J. King

  5. Seminis Vegetable Seeds, Mas de Rouzel, Chemin des Canaux, 30900, Nîmes, France

    F. Bertrand

  6. Vegetable Crops Unit, Agricultural Research Service, US Department of Agriculture, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA

    M. Havey

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  1. Y. Park
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  2. N. Katzir
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  3. Y. Brotman
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  4. J. King
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  5. F. Bertrand
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  6. M. Havey
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Correspondence to M. Havey.

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Communicated by C. Möllers

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Park, Y., Katzir, N., Brotman, Y. et al. Comparative mapping of ZYMV resistances in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). Theor Appl Genet 109, 707–712 (2004). https://doi.org/10.1007/s00122-004-1684-y

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  • DOI: https://doi.org/10.1007/s00122-004-1684-y

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