Abstract
Wild perennial Glycine species are an invaluable gene resource for the cultivated soybean [Glycine max (L.) Merr., 2n=40]. However, these wild species have been largely unexplored in soybean breeding programs because of their extremely low crossability with soybean and the need to employ in vitro embryo rescue methods to produce F1 hybrids. The objective of this study was to develop molecular markers to identify gene introgression from G. tomentella, a wild perennial Glycine species, to soybean. A selection of 96 soybean simple sequence repeat (SSR) markers was evaluated for cross-specific amplification and polymorphism in G. tomentella. Thirty-two SSR markers (33%) revealed specific alleles for G. tomentella PI 483218 (2n=78). These SSR markers were further examined with an amphidiploid line (2n=118) and monosomic alien addition lines (MAALs), each with 2n=40 chromosomes from soybean and one from G. tomentella. The results show that the use of SSR markers is a rapid and reliable method to detect G. tomentella chromosomes in MAALs. We also developed a cleaved amplification polymorphism sequence (CAPS) marker according to the sequences of internal transcribed spacer (ITS) regions in soybean and G. tomentella. Four MAALs that carry the ITS (rDNA) locus from G. tomentella were identified. The SSR and ITS-CAPS markers will greatly facilitate the introgression and characterization of gene transfer from G. tomentella to soybean.
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Bommineni VR, Jauhar PP, Peterson TS, Chibbar RN, Almouslem AB (1997) Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers. Theor Appl Genet 95:757–763
Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, VanToai TT, Lones DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490
Cregan PB, Kollipara KP, Xu SJ, Singh RJ, Hymowitz T (2001) Primary trisomics and SSR markers as tools to associate chromosomes with linkage groups in soybean. Crop Sci 41:1262–1267
Demirbas A, Rector BG, Lohnes DG, Fioritto RJ, Graef GL, Cregan PB, Shoemaker RC, Specht JE (2001) Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Theor Appl Genet 41:1220–1227
Doyle MJ, Brown AHD (1985) Numerical analysis of isozyme variation in Glycine tomentella. Biochem Syst Ecol 13:413–419
Escalante A, Imanishi S, Hossain M, Ohmido N, Fukui K (1998) RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides. Theor Appl Genet 96:719–726
Hernandez P, Laurie DA, Martin A, Snape JW (2002) Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum. Theor Appl Genet 104:735–739
Hymowitz T, Singh RJ, Kollipara KP (1998) The genomes of the Glycine. Plant Breed Rev 16:289–317
Kollipara KP, Singh RJ, Hymowitz T (1994) Genomic diversity and multiple origins of tetraploid (2n=78, 80) Glycine tomentella Hayata. Genome 37:448–459
Kollipara, KP, Singh RJ, Hymowitz T (1997) Phylogenetic and genomic relationships in the genus Glycine Willd. based on sequences from the ITS region on nuclear rDNA. Genome 40:57–68
Marek LF, Mudge J, Darnielle L, Grant D, Hanson N, Paz M, Yan HH, Denny R, Larson K, Foster-Hartnett D, Cooper A, Danesh D, Larsen D, Schmidt T, Staggs R, Crow JA, Retzel E, Young ND, Shoemaker RC (2001) Soybean genomic survey: BAC-end sequences near RFLP and SSR markers. Genome 44:572–581
Mian MAR, Wang TY, Phillips DV, Alvernaz J, Boerman HR (1999) Molecular mapping of the Rcs3 gene for resistance to frogeye leaf spot in soybean. Crop Sci 39:1687–1691
Orf JH, Chase K, Jarvik T, Mansur LM, Cregan PB, Adler FR, Lark KG (1999) Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci 39:1642–1651
Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol Biol Evol 15:1275–1287
Peil A, Schubert V, Schumann E, Weber WE (1997) RAPDs as molecular markers for the detection of Aegilops markgrafi chromatin in addition and euploid introgression lines of hexaploid wheat. Theor Appl Genet 94:934–940
Rauscher JT, Doyle JJ, Brown AHD (2004) Multiple origin and nrDNA internal transcribed spacer homologue evolution in the Glycine tomentella (leguminosae) allopolyploid complex. Genetics 166:987–998
Riggs RD, Wang S, Singh RJ, Hymowitz T (1998) Possible transfer of resistance to Herterodera glycines from Glycine tomentella to Glycine max. J Nematol [Suppl] 30:547–552
Rossetto M (2001) Sourcing of SSR marker from related plant species. In: Henry RJ (ed) Plant genotyping: the DNA fingerprinting of plants. CAB International, Wallingford, pp 211–224
Schoen DJ, Burdon JJ, Brown AHD (1992) Resistance of Glycine tomentella to soybean leaf rust Phakopsora pachyrhiz in relation to ploidy level and geographic distribution. Theor Appl Genet 83:827–832
Singh RJ, Hymowitz T (1988) The genomic relationship between Glycine max (L.) Merr and G. soja Sieb. and Zucc. revealed by pachytene analysis. Theor Appl Genet 76:705–711
Singh RJ, Hymowitz T (1999) Soybean genetic resources and crop improvement. Genome 42:605–616
Singh RJ, Kollipara KP, Hymowitz T (1998) Monosomic alien addition lines derived from Glycine max (L.) Merr. and G. tomentella Hayata: production, characterization and breeding behavior. Crop Sci 38:1483–1489
Singh RJ, Kim HH, Hymowitz T (2001) Distribution of rDNA loci in the genus Glycine Willd. Theor Appl Genet 103:212–218
Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet (in press)
Walbot V (1988) Preparation of DNA from single rice seedlings. Rice Genet Newsl 5:149–151
Yan HH, Liu GQ, Cheng ZK, Min SK, Zhu LH (2001) Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and Oeichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH). Genome 44:86–95
Zou JJ, Singh RJ, Lee J, Xu SJ, Cregan PB, Hymowitz T (2003) Assignment of molecular linkage groups to soybean chromosomes by primary trisomics. Theor Appl Genet 107:745–750
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This research was supported in part by Illinois Agriculture Experimental Station.
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Communicated by F.J. Muehlbauer
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Zou, J.J., Singh, R.J. & Hymowitz, T. SSR marker and ITS cleaved amplified polymorphic sequence analysis of soybean × Glycine tomentella intersubgeneric derived lines. Theor Appl Genet 109, 769–774 (2004). https://doi.org/10.1007/s00122-004-1680-2
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DOI: https://doi.org/10.1007/s00122-004-1680-2