Skip to main content
SpringerLink
Log in

Self-incompatibility genotypes in almond re-evaluated by PCR, stylar ribonucleases, sequencing analysis and controlled pollinations

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

As part of the almond breeding programme at IRTA, we investigated the S genotypes of several cultivars using a combination of RNase zymograms, testcrosses, pollen-tube growth analysis and molecular identification by PCR analysis. For some of the cultivars examined, discrepancies appeared between their S alleles as reported in the literature and those found in this investigation, leading to a re-evaluation of their S genotypes. Analysis of the stylar ribonucleases (RNases), which are known to correlate with S alleles, of cvs. Achaak, Ardechoise, Desmayo Largueta, Ferrastar, Gabaix, Garbí, Glorieta, Languedoc, Primorskiy and Texas revealed inconsistencies with respect to the S5 and S10 alleles. However, PCR with the conserved primer pair AS1II/AmyC5R failed to detect any of these inconsistencies. When the S alleles from Desmayo Largueta, Gabaix, Primorskiy and Texas were sequenced, Texas and Primorskiy were found to carry the reported S5 allele, while Desmayo Largueta and Gabaix carried a new allele, which has been tentatively denoted as S25 This new S allele, previously reported to be S10, was also identified in Achaak, Ardechoise and Ferrastar. The proposed new S genotypes are Achaak (S2S25), Ardechoise (S1S25), Desmayo Largueta (S1S25), Ferrastar (S2S25) and Gabaix (S10S25). The S alleles of Garbí, Glorieta, Languedoc, Texas and Primorskiy remain as reported in the literature. Testcrosses in the field and laboratory confirmed the new S genotypes. One cultivar (Gabaix) could be assigned to the existing cross-incompatibility group O of unique genotypes, and two new groups were established (XVI and XVII) consisting of two cultivars each. The clarification of these S alleles will be useful in almond breeding programmes and for planning new commercial orchards in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Arús P, Olarte C, Romero M, Vargas FJ (1994) Linkage analysis of ten isozyme genes in F1 segregating almond progenies. J Am Soc Hortic Sci 119:339-344

    Google Scholar 

  • Batlle I, Ballester J, Boskovic R, Romero MA, Tobutt KR, Vargas FJ (1997) Use of stylar ribonucleases in almond breeding to design crosses and select self-compatible seedlings. FAO-CIHEAM Netw Nuts Nucis Newsl 6:12-14

  • Boskovic R, Tobutt KR, Batlle I, Duval H (1997) Correlation of ribonuclease zymograms and incompatibility genotypes in almond. Euphytica 97:167-176

    Article  CAS  Google Scholar 

  • Boskovic R, Tobutt KR, Rovira M, Romero MA, Batlle I, Duval H, Dicenta F (1998) Inheritance of stylar ribonucleases in two almond progenies and their correlation with self-compatibility. In: Proc 2nd Int Symp Pistachios Almonds. Acta Hortic 470:118-122

    CAS  Google Scholar 

  • Boskovic R, Tobutt KR, Duval H, Batlle I, Dicenta F, Vargas FJ (1999) A stylar ribonuclease assay to detect self-compatible seedlings in almond progenies. Theor Appl Genet 99:800-810

    Article  CAS  Google Scholar 

  • Boskovic R, Tobutt KR, Batlle I, Duval H, Martínez-Gómez P, Gradziel TM (2003) Stylar ribonucleases in almond: correlation with and prediction of incompatibility genotypes. Plant Breed 122:70-76

    Article  CAS  Google Scholar 

  • Broothhaerts W (2003) New findings in apple S-genotype analysis resolve previous confusion and request the re-numbering of some S-alleles. Theor Appl Genet 106:703-714

    PubMed  Google Scholar 

  • Channuntapipat C, Sedgley M, Collins G (2001) Sequences of cDNAs and genomic DNAs encoding the S1, S7, S8 and Sf alleles from almond, Prunus dulcis. Theor Appl Genet 103:1115-1122

    Article  CAS  Google Scholar 

  • Channuntapipat C, Collins G, Sedgley M (2002a) Self-compatibility of almond varieties Australian Nutgrower Sept-Nov 2002, pp 40-41

  • Channuntapipat C, Sedgley M, Batlle I, Arús P, Collins G (2002b) Sequences of the genomic DNAs encoding the S2, S9, S10 and S23 alleles from almond, Prunus dulcis. J Hortic Sci Biotechnol 77:387-392

    CAS  Google Scholar 

  • Channuntapipat C, Wirthensohn M, Ramesh SA, Batlle I, Arús, P, Sedgley M, Collins G (2003) Identification of incompatibility genotypes in almond (Prunus dulcis Mill.) using specific primers based on the introns of the S-alleles. Plant Breed 122:164-168

    CAS  Google Scholar 

  • Crossa-Raynaud P, Grasselly C (1985) Existence de groupes dinterstérilité chez lamandier. Coll GREMPA Thessalonique. Options Mediterr 1985-I:43-45

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11-15

    Google Scholar 

  • Duval H, Batlle I, Boskovic R, Tobbut KR (1998) Confirmation of incompatibility alleles in almond cultivars predicted by analysis of stylar ribonucleases. In: 10th GREMPA Seminar. Options Mediterr 33:25-27

    Google Scholar 

  • Felipe AJ (1977) Estados fenológicos del almendro. Inf Tec Econ Agrar 8:8-9

    Google Scholar 

  • Grasselly C (1972) Lamandier: caractères morphologiques et physiologiques des variétés, modalité de leurs transmissions chez les hybrides de première génération. PhD, Université de Bordeaux, Bordeaux, France

  • Hall TA (1999) bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95-99

    CAS  Google Scholar 

  • Heslop-Harrison J, Heslop-Harrison Y, Shivanna KR (1984) The evaluation of pollen quality, and a further appraisal of the fluorochromatic (FCR) test procedure. Theor Appl Genet 67:367-375

    Google Scholar 

  • Ishimizu T, Endo T, Yamaguchi-Kabata Y, Makamura KT, Sakiyama F, Norioka S (1998) Identification of regions in which positive selection may operate in S-RNase of Rosaceae: implication for S-allele-specific recognition sites in S-RNase. FEBS Lett 440:337-342

    Article  CAS  PubMed  Google Scholar 

  • Kester DE, Asay RA (1975) Almonds. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University Press, Indianapolis, pp 387-419

  • Kester DE, Gradziel TM, Micke WC (1994a) Identifying pollen incompatibility groups in California almond cultivars. J Am Soc Hortic Sci 119:106-109

    Google Scholar 

  • Kester DE, Micke WC, Viveros M (1994b) A mutation in Nonpareil almond conferring unilateral incompatibility. J Am Soc Hortic Sci 119:1289-1292

    Google Scholar 

  • Kester DE, Gradziel TM (1996) Almonds (Prunus). In: Moore JN, Janick J (eds) Fruit breeding. Wiley, New York, pp 1-97

  • López M, Alonso JM, Martínez-Gómez P, Socías I Company R, Gradziel TM, Batlle I (2001) Biological and molecular assessment of self-incompatibility in almond. FAO-CIHEAM Netw Nuts Nucis Newslett 10:8-11

  • López M, Mnejja M, Romero MA, Vargas FJ, Batlle I (2004) Use of Sf specific PCR for early selection of self-compatible seedlings in almond breeding. In: 12th GREMPA Meet. Mirandela (in press)

  • Ma R, Oliveira MM (2001) Molecular cloning of the self-incompatibility genes S1 and S3 from almond (Prunus dulcis cv. Ferragnès). Sex Plant Reprod 14:163-167

    Article  CAS  Google Scholar 

  • Martínez-Gómez P, López M, Alonso JM, Ortega E, Batlle I, Socías and Company R, Dicenta F, Dandekar AM, Gradziel TM (2003a) Identification of self-incompatibility alleles in almond and related Prunus species using PCR. In: 26th ISHS Int Hortic Congr. Acta Hortic 622:397-401

    Google Scholar 

  • Martínez-Gómez P, Arulsekar S, Potter D, Gradziel TM (2003b) An extended interspecific gene pool available to peach and almond breeding as characterized using simple sequence repeat (SSR) markers. Euphytica 131:313-322

    Article  Google Scholar 

  • Mnejja M, López M, Romero MA, Vargas FJ, Batlle I (2002) Cross design for self-compatibility in almond breeding using stylar ribonucleases. In: 3rd Int Symp Pistachios Almonds and 12th GREMPA Colloq. Acta Hortic 591:233-238

    CAS  Google Scholar 

  • Ortega E (2002) Mejora genética del almendro: nuevas estrategias para la obtención de variedades autocompatibles. CSIC, Centro de Edafología y Biología Aplicada del Segura. PhD thesis, Universidad de Murcia, Spain

  • Ortega E, Dicenta F (2003) Inheritance of self-compatibility in almond: breeding strategies to assure self-compatibility in the progeny. Theor Appl Genet 106:904-911

    CAS  PubMed  Google Scholar 

  • Page RDM (1996) Treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357-358

    CAS  PubMed  Google Scholar 

  • Rovira M, Clavé J, Romero M, Santos J, Vargas FJ (1998) Self-compatibility in almond progenies. In: Proc 2nd Int Symp Pistachios Almonds. Acta Hortic 470:66-71

    Google Scholar 

  • Sonneveld T, Tobutt KR, Robbins TP (2003) Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers. Theor Appl Genet 107:1059-1070

    Article  CAS  PubMed  Google Scholar 

  • Tamura M, Ushijima K, Sassa H, Hirano H, Tao R, Gradziel TM, Dandekar AM (2000) Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis. Theor Appl Genet 101:344-349

    Article  CAS  Google Scholar 

  • Tao R, Yamane H, Sassa H, Mori H, Gradziel TM, Dandekar AM, Sugiura A (1997) Identification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis). Plant Cell Physiol 38:304-311

    CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The clustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876-4882

    Article  Google Scholar 

  • Van Nerum I, Geerts M, Van Haute A, Keulemans J, Broothaerts W (2001) Re-examination of the self-incompatibility genotype of apple cultivars containing putative new S-alleles. Theor Appl Genet 103:584-591

    Google Scholar 

  • Vargas FJ (1975) El almendro en la provincia de Tarragona. Diputación Provincial de Tarragona

  • Viruel MA (1995) Construcción de un mapa genético con marcadores moleculares en almendro (Prunus amygdalus Batsch.). Estudio de la variabilidad intraespecífica y aplicación a la identificación varietal. PhD thesis, IRTA, Departament de Genètica Vegetal, Universitat de Barcelona, Barcelona, Spain

  • Wiersma PA, Wu Z, Zhou L, Hampson C, Kappel F (2001) Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis. Theor Appl Genet 102:700-708

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to P. Martínez-Gómez (CEBAS, CSIC, Murcia) and T.M. Gradziel (University of California, Davis, USA) for their valuable comments and suggestions. We also thank J. Clavé, M.A. Romero, J. Santos and J. Vergés (IRTA-Mas Bové), D. Gregory and K. Malone (University of Adelaide, Australia) and members of the laboratory of Genètica Vegetal at IRTA-Cabrils for their help with the field and laboratory work. This research was conducted under two INIA (Spanish Ministry of Education and Science) Projects (SC97-049 and RTA01-081). M.L. gratefully acknowledges a grant from the INIA postgraduate programme.

Author information

Authors and Affiliations

  1. Departament d’Arboricultura Mediterrània, IRTA-Centre de Mas Bové, Ap. 415, 43280, Reus-Tarragona, Spain

    Mercè López, Mercè Rovira, Francisco J. Vargas & Ignasi Batlle

  2. Departament de Genètica Vegetal, IRTA-Centre de Cabrils, Ctra Cabrils s/n, 08348, Cabrils-Barcelona, Spain

    Mourad Mnejja & Pere Arús

  3. Department of Horticulture, Viticulture and Oenology, University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia

    Graham Collins

Authors
  1. Mercè López
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mourad Mnejja
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mercè Rovira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Graham Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Francisco J. Vargas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pere Arús
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ignasi Batlle
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ignasi Batlle.

Additional information

Communicated by H.F. Linskens

Rights and permissions

Reprints and permissions

About this article

Cite this article

López, M., Mnejja, M., Rovira, M. et al. Self-incompatibility genotypes in almond re-evaluated by PCR, stylar ribonucleases, sequencing analysis and controlled pollinations. Theor Appl Genet 109, 954–964 (2004). https://doi.org/10.1007/s00122-004-1656-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1656-2

Keywords

Search

Navigation