Skip to main content
SpringerLink
Log in

Identification and mapping of resistance gene analogs and a white rust resistance locus in Brassica rapa ssp. oleifera

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The objective of this investigation was to tag a locus for white rust resistance in a Brassica rapa ssp. oleifera F2 population segregating for this trait, using bulked segregant analysis with random amplified polymorphic DNA (RAPD) markers, linkage mapping and a candidate gene approach based on resistance gene analogs (RGAs). The resistance source was the Finnish line Bor4109. The reaction against white rust races 7a and 7v was scored in 20 seedlings from each self-pollinated F2 individual. The proportion of resistant plants among these F3 families varied from 0 to 67%. Bulked segregant analysis did not reveal any markers linked with resistance and, therefore, a linkage map with 81 markers was created. A locus that accounted for 18.4% of the variation in resistance to white rust was mapped to linkage group (LG) 2 near the RAPD marker Z19a. During the study, a bacterial resistance gene homologous to Arabidopsis RPS2 and six different RGAs were sequenced. RPS2 and five of the RGAs were mapped to linkage groups LG1, LG4 and LG9. Unfortunately, none of the RGAs could be shown to be associated with white rust resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Aarts MGM, te Lintel Hekkert B, Holub EB, Beynon JL, Stiekema WJ, Pereira A (1998) Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana. Mol Plant-Microb Interact 11:251–258

    Google Scholar 

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped blast and psi-blast: a new generation of protein database search programmes. Nucleic Acids Res 25:3389–3402

    PubMed  Google Scholar 

  • Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt R, Giraudat J, Leung J, Staskawicz BJ (1994) RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265:1856–1860

    CAS  PubMed  Google Scholar 

  • Bernier CC (1972) Diseases of rapeseed in Manitoba in 1971. Can Plant Dis Surv 52:108

    Google Scholar 

  • Collins N, Park R, Spielmeyer W, Ellis J, Pryor AJ (2001) Resistance gene analogs in barley and their relationship to rust resistance genes. Genome 44:375–381

    Article  CAS  PubMed  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Rep 1:19–21

    CAS  Google Scholar 

  • Delwiche PA, Williams PH (1974) Resistance to Albugo candida race 2 in Brassica sp. Proc Am Phytopathol Soc 1:66

    Google Scholar 

  • Donald TM, Pellerone F, Adam-Blondon A-F, Bouquet A, Thomas MR, Dry IB (2002) Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. Theor Appl Genet 104:610–618

    CAS  Google Scholar 

  • Ebrahimi AG, Delwiche PA, Williams PH (1976) Resistance in Brassica juncea to Peronospora parasitica and Albugo candida race 2. Proc Am Phytopathol Soc 3:273–273

    Google Scholar 

  • Edwards MD, Williams PH (1987) Selection for minor gene resistance to Albugo candida in a rapid-cycling population of Brassica campestris. Phytopathology 77:527–532

    Google Scholar 

  • Fan Z, Rimmer SR, Stefansson BR (1983) Inheritance of resistance to Albugo candida in rape (Brassica napus L.). Can J Genet Cytol 25:420–424

    Google Scholar 

  • Ferreira ME, Williams PH, Osborn TC (1995) Mapping of a locus controlling resistance to Albugo candida in Brassica napus using molecular markers. Phytopathology 85:218–220

    CAS  Google Scholar 

  • Fourmann M, Charlot F, Froger N, Delourme R, Brunel D (2001) Expression, mapping, and genetic variability of Brassica napus disease resistance gene analogues. Genome 44:1083–1099

    Article  CAS  PubMed  Google Scholar 

  • Gawel M, Wisniewska I, Rafalski A (2002) Semi-specific PCR for the evaluation of diversity among cultivars of wheat and triticale. Cell Mol Biol Lett 7:577–582

    CAS  PubMed  Google Scholar 

  • Gedil MA, Slabaugh MB, Berry S, Johnson R, Michelmore R, Miller J, Gulya T, Knapp SJ (2001) Candidate disease resistance genes in sunflower cloned using conserved nucleotide-binding site motifs: Genetic mapping and linkage to the downy mildew resistance gene Pl1. Genome 44:205–212

    Article  CAS  PubMed  Google Scholar 

  • Grant MR, Godiard L, Straube E, Ashfield T, Lewald J, Sattler A, Innes RW, Dangl JL (1995) Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance. Science 269:843–846

    CAS  PubMed  Google Scholar 

  • Haley SD, Afanador L, Kelly JD (1994) Selection for monogenic pest resistance traits with coupling- and repulsion-phase RAPD markers. Crop Sci 34:1061–1066

    Google Scholar 

  • Hammond-Kosack KE, Jones JDG (1997) Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol Biol 48:575–607

    CAS  Google Scholar 

  • Hill CB, Crute IR, Sherriff C, Williams PH (1988) Specificity of Albugo candida and Peronospora parasitica pathotypes toward rapid-cycling crucifers. Cruciferae Newsl 13:112–113

    Google Scholar 

  • Joyeux A, Fortin MG, Mayerhofer R, Good AG (1999) Genetic mapping of plant disease resistance gene homologues using a minimal Brassica napus L. population. Genome 42:735–743

    CAS  Google Scholar 

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci 93:11746–11750

    CAS  PubMed  Google Scholar 

  • Kole C, Teutonico R, Mengistu A, Williams PH, Osborn TC (1996) Molecular mapping of a locus controlling resistance to Albugo candida in Brassica rapa. Phytopathology 86:367–369

    CAS  Google Scholar 

  • Kole C, Williams PH, Rimmer SR, Osborn TC (2002) Linkage mapping of genes controlling resistance to white rust (Albugo candida) in Brassica rapa (syn. campestris) and comparative mapping to Brassica napus and Arabidopsis thaliana. Genome 45:22–27

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lawrence GJ, Finnegan EJ, Aycliffe MA, Ellis JG (1995) The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. Plant Cell 7:1195–1206

    CAS  PubMed  Google Scholar 

  • Leister D, Ballvora A, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat Genet 14:421–429

    CAS  PubMed  Google Scholar 

  • Leister D, Kurth J, Laurie DA, Yano M, Sasaki T, Graner A, Schulze-Lefert P (1999) RFLP- and physical mapping of resistance gene homologues in rice (O.sativa) and barley (H.vulgare). Theor Appl Genet 98:509–520

    CAS  Google Scholar 

  • Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461

    Article  CAS  Google Scholar 

  • Liu JQ, Parks P, Rimmer SR (1996) Development of monogenic lines for resistance to Albugo candida from a Canadian Brassica napus cultivar. Phytopathology 86:1000–1004

    Google Scholar 

  • Liu Q, Rimmer SR (1991) Inheritance of resistance in Brassica napus to an Ethiopian isolate of Albugo candida from Brassica carinata. Can J Plant Pathol 13:197–290

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Meyers BC, Dickerman AW, Michelmore RW, Sivaramakrishnan S, Sobral BW, Young ND (1999) Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J 20:317–332

    Article  CAS  PubMed  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Genetics 88:9828–9832

    CAS  Google Scholar 

  • Peñuela S, Danesh D, Young ND (2002) Targeted isolation, sequence analysis, and physical mapping of nonTIR NBS-LRR genes in soybean. Theor Appl Genet 104:261–272

    Article  Google Scholar 

  • Petrie GA (1988) Races of Albugo candida (white rust and staghead) on cultivated Cruciferae in Saskatchewan. Can J Plant Pathol 10:142–150

    Google Scholar 

  • Pidskalny RS, Rimmer SR (1985) Virulence of Albugo candida from turnip rape (Brassica campestris) and mustard (Brassica juncea) on various crucifers. Can J Plant Pathol 7:283–286

    Google Scholar 

  • Pound GS, Williams PH (1963) Biological races of Albugo candida. Phytopathology 53:1146–1149

    Google Scholar 

  • Prabhu KV, Somers DJ, Rakow G, Gugel RK (1998) Molecular markers linked to white rust resistance in mustard Brassica juncea. Theor Appl Genet 97:865–870

    Article  CAS  Google Scholar 

  • Saraste M, Sibbald PR, Wittinghofer A (1990) The P-loop—a common motif in ATP- and GTP-binding proteins. Trends Biochem Sci 15:430–434

    PubMed  Google Scholar 

  • Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516

    CAS  PubMed  Google Scholar 

  • Somers DJ, Rakow G, Rimmer SR (2002) Brassica napus DNA markers linked to white rust resistance in Brassica juncea. Theor Appl Genet 104:1121–1124

    Article  Google Scholar 

  • Speulman E, Bouchez D, Holub EB, Beynon JL (1998) Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. Plant J 14:467–474

    CAS  PubMed  Google Scholar 

  • Staskawicz BJ, Ausubel FM, Baker BJ, Ellis JG, Jones JDG (1995) Molecular genetics of plant disease resistance. Science 268:661–667

    CAS  PubMed  Google Scholar 

  • Szewc-McFadden AK, Kresovich S, Bliek SM, Mitchell SE, McFerson JR (1996) Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species. Theor Appl Genet 93:534–538

    CAS  Google Scholar 

  • Tanhuanpää P, Vilkki JP, Vilkki HJ (1993) Genetic polymorphism at RAPD loci in spring turnip rape (Brassica rapa ssp. oleifera). Agric Sci Finl 2:303–310

    Google Scholar 

  • Tanhuanpää PK, Vilkki JP, Vilkki HJ (1996) A linkage map of spring turnip rape based on RFLP and RAPD markers. Agric Food Sci Finl 5:209–217

    Google Scholar 

  • Tanhuanpää P, Vilkki J, Vihinen M (1998) Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. oleifera). Mol Breed 4:543–550

    Article  Google Scholar 

  • Teutonico RA, Osborn TC (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89:885–894

    CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) clustal w: improving the sensitivity progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight metrix choice. Nucleic Acids Res 22:4673–4680

    PubMed  Google Scholar 

  • Tiwari AS, Petrie GA, Downey RK (1988) Inheritance of resistance to Albugo candida race 2 in mustard [Brassica Juncea (L.) Czern.]. Can J Plant Sci 68:297–300

    Google Scholar 

  • Toojinda T, Broers LH, Chen XM, Hayes PM, Kleinhofs A, Korte J, Kudrna D, Leung H, Line RF, Powell W, Ramsay L, Vivar H, Waugh R (2000) Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). Theor Appl Genet 101:580–589

    CAS  Google Scholar 

  • Traut TW (1994) The functions and consensus motifs of nine types of peptide segments that form different types of nucleotide-binding sites. Eur J Biochem 222:9–19

    CAS  PubMed  Google Scholar 

  • Verma U, Bhowmik TP (1989) Inheritance of resistance to a Brassica juncea pathotype of Albugo candida in B. napus. Can J Plant Pathol 11:443–444

    Google Scholar 

  • Vicente JG, King GJ (2001) Characterisation of disease resistance gene-like sequences in Brassica oleracea L. Theor Appl Genet 102:555–563

    CAS  Google Scholar 

  • Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the Interleukin-1 receptor. Cell 78:1101–1115

    CAS  PubMed  Google Scholar 

  • Yu YG, Buss GR, Maroof MAS (1996) Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site. Proc Natl Acad Sci 93:11751–11756

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The author wishes to thank Mr. Juha Vilkki, the breeder of spring turnip rape, for providing the plant material for the study. She is also grateful to Mr. Asko Hannukkala and Ms. Mari Helminen for disease inoculations, Ms. Leena Lamminpää and Ms. Aija Viljanen for growing the plants, making the pollinations and checking the disease evaluations. She also warmly thanks Ms. Marja-Riitta Arajärvi for skillful technical assistance and Ms. Anneli Virta for DNA sequencing. Ms. Ritva Koskenoja is thanked for making the reference list and Prof. Alan Schulman for revising the manuscript.

Author information

Authors and Affiliations

  1. Crops and Biotechnology, Plant Production Research, MTT/Agrifood Research Finland, 31600, Jokioinen, Finland

    P. Tanhuanpää

Authors
  1. P. Tanhuanpää
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Tanhuanpää.

Additional information

Communicated by H.C. Becker

The nucleotide sequence data reported has been deposited in the Genbank under the accession numbers AF315081–AF315087.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanhuanpää, P. Identification and mapping of resistance gene analogs and a white rust resistance locus in Brassica rapa ssp. oleifera . Theor Appl Genet 108, 1039–1046 (2004). https://doi.org/10.1007/s00122-003-1525-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1525-4

Keywords

Search

Navigation