Abstract
The potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species was evaluated. A set of 25 barley and 3 wheat microsatellite markers were tested for their ability to cross-amplify DNA from four accessions of E. trachycaulus and two accessions Pseudoroegneria spicata. Thirteen barley (52%) and two (68%) wheat primer pairs successfully amplified consistent products from both E. trachycaulus and P. spicata species. Four of the 15 successful primer pairs produced visible polymorphisms among the accessions tested. A higher successful rate of cross-species amplification of barley and wheat microsatellite markers in E. trachycaulus and P. spicata was found in this study. These primer pairs are now available for use as markers in genetic analysis of E. trachycaulus complex species. Our results suggest that publicly available wheat and barley microsatellite markers are a valuable resource for the genetic characterization of wild Triticeae species.
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References
Aung T (1991) Intergeneric hybrids between Hordeum vulgare and Elymus trachycaulus resistant to Russian wheat aphid. Genome 34:954–960
Barkworth ME (1994) The Elymus trachycaulus complex in North America: more questions than answers. In: Wang RRC, Jensen KB, Jaussi C (eds) Proc 2nd Int Triticeae Symp, Logan, Utah, USA, pp189–198
Brown RW, Johnston RS, Johnson DA (1978) Rehabilitation of alpine tundra disturbances. J Soil Water Conserv 33:154–160
Clauss MJ, Cobban H, Mitchell-olds T (2002) Cross-species microsatellite markers for elucidating population genetic structure in Arabidopsis and Arabis (Brassicaeae). Mol Ecol 11:591–601
Dewey DR (1968) Synthetic Agropyron-Elymus hybrids. III. Elymus canadensis x Agropyron caninum, A. trachycaulum, and A. striatum. Am J Bot 55:1133–1139
Dewey DR (1975) Introgression between A. dasystachyum and A. trachycaulum. Bot Gaz 136:122–128
Dewey DR (1982) Genomic and phylogenetic relationships among North American perennial Triticeae. In: Estes JE (ed) Grasses and grassland. University of Oklahoma Press, Oklahoma, pp 51–88
Fitzsimmons NN, Moritz C, Moore SS (1995) Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Mol Biol Evol 12:432–440
Hernández P, Laurie DA, Martin A, Snape JW (2002) Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum. Theor Appl Genet 104:735–739
Hitchcock AS (1951) Manual of grasses of the United States, 2nd edn. USDA Misc Publ 200
Junghans H, Metzlaff M (1990) A simple and rapid method for the preparation of total plant DNA. BioTechniques 8:176
Liu ZW, Biyashez RM, Saghai Maroof MA (1996) Development of simple sequence repeat DNA makers and their integration into barley linkage map. Theor Appl Genet 93:869–876
Moore G (1995) Cereal genome evolution: pastoral pursuits with “Lego” genomes. Curr Opin Genet Dev 5:717–724
Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSR) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol Biol Evol 15:1275–1287
Röder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333
Sun GL, Salomon B, Bothmer RV (1997) Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD makers. Genome 40:806–814
Sun GL, Salomon B, Bothmer RV (1998a) Characterization and analysis of microsatellite loci in Elymus caninus (Triticeae: Poaceae). Theor Appl Genet 96:676–682
Sun GL, Salomon B, Bothmer von R (1998b) Characterization of microsatellite loci from Elymus alaskanus and length polymorphism in several Elymus species (Triticeae: Poaceae). Genome 41:455–463
Sun GL, Diaz O, Salomon B, Bothmer RV (1998c) Microsatellite variation and its comparison with allozyme and RAPD variation in Elymus fibrosus (Schrenk) Tzvel. (Poaceae). Hereditas 129:275–282
Sun GL, Díaz O, Salomon B, Bothmer RV (1999) Genetic diversity in Elymus caninus as revealed by isozyme, RAPD and microsatellite markers. Genome 42, 420–431
Sun GL, Díaz O, Salomon B, Bothmer RV (2001) Genetic diversity and structure in a natural Elymus caninus population from Denmark based on microsatellite and isozyme analysis. Plant Syst Evol 227:235–244
Sun GL, Salomon B, Bothmer RV (2002) Microsatellite polymorphism and genetic differentiation in three Norwegian populations of Elymus alaskanus (Poaceae). Plant Syst Evol 234:101–110
Treuren R van, Kuittinen H, Karkkainen K, Baena-Gonzalez E, Savolainen O (1997) Evolution of microsatellites in Arabis petraea and Arabis lyrata, outcrossing relatives of Arabidopsis thaliana. Mol Biol Evol 14:220–229
Wang RRC, Bothmer RV, Dvorak J, Fedak G, Linde-Laursen I, Muramatsu M (1994) Genome symbols in the Triticeae (Poaceae). In: Wang RRC, Jensen KB, Jaussi C (eds) Proc 2nd Int Triticeae Symp, Logan Utah, USA, pp 29–34
Acknowledgements
The authors thank the Natural Sciences and Engineering Research Council of Canada (NSERC) for funding this work. Support from a Saint Mary’s University Senate grant is gratefully acknowledged. The seeds used in this study were kindly provided by the United States Department of Agriculture (USDA).
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Communicated by H.F. Linskens
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MacRitchie, D., Sun, G. Evaluating the potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species. Theor Appl Genet 108, 720–724 (2004). https://doi.org/10.1007/s00122-003-1472-0
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DOI: https://doi.org/10.1007/s00122-003-1472-0