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Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth.

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Abstract

Leaf rust, caused by Puccinia hordei, is an important disease afflicting barley (Hordeum vulgare) in many production regions of the world. The leaf rust resistance gene Rph15 was identified in an accession of wild barley (Hordeum vulgare subsp. spontaneum) and is one of the most broadly effective resistance genes known. Using amplified fragment length polymorphism (AFLP) and simple sequence repeat markers, Rph15 was mapped to chromosome 2HS in an F2 population derived from a cross between Bowman (Rph15), a Bowman backcross-derived line carrying Rph15, and the susceptible cultivar Bowman. AFLP marker P13M40 co-segregated with Rph15 in this mapping population and two others involving Bowman (Rph15) and cultivars Proctor and Nudinka. The dominant AFLP marker P13M40 was converted to a co-dominant PCR-based marker that may be useful in breeding programs employing marker-assisted selection. The allelic relationship between Rph15 and the gene Rph16, also mapping to chromosome 2HS, was studied. The lack of segregation in F2 progeny derived from the two resistance sources indicates that Rph15 and Rph16 are alleles of the same locus.

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Acknowledgements

J.S.W. was supported by the SAREC grant for capacity building in biotechnology awarded to Prof. Eric Karunanayake, University of Colombo, Sri Lanka. Drs. J.D. Franckowiak, Andreas Graner and A.K.M.R. Islam are gratefully acknowledged for gifts of seeds. A.B. Falk was supported by the Research Council for Agriculture and Forestry (SJFR). B. Steffenson was supported in part by the American Malting Barley Association and the Lieberman-Okinow Endowment.

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  1. Jagathpriya S. Weerasena

    Present address: Department of Biochemistry and Molecular Biology, University of Colombo, Colombo, Sri Lanka

Authors and Affiliations

  1. Department of Plant Biology, Swedish University of Agricultural Sciences, PO Box 7080, 75007, Uppsala, Sweden

    Jagathpriya S. Weerasena & Anders B. Falk

  2. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108–6030, USA

    Brian J. Steffenson

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  1. Jagathpriya S. Weerasena
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Correspondence to Anders B. Falk.

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Communicated by F. Salamini

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Weerasena, J.S., Steffenson, B.J. & Falk, A.B. Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth.. Theor Appl Genet 108, 712–719 (2004). https://doi.org/10.1007/s00122-003-1470-2

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