Abstract
Abscission is a universal process whereby plants shed their organs, such as flowers, fruit and leaves. In tomato, the non-allelic mutations jointless and jointless-2 have been discovered as recessive mutations that completely suppress the formation of pedicel abscission zones. A high resolution genetic map of jointless-2 was constructed using 1,122 jointless F2 plants. Restriction fragment length polymorphism (RFLP) marker RPD140 completely co-segregated with the jointless-2 locus and mapped in a 2.4 cM interval between RFLP markers CD22 and TG618. To chromosome walk to jointless-2, all three markers were used to screen a bacterial artificial chromosome (BAC) library and contigs were developed. Intensive efforts to expand and merge the BAC contigs were unsuccessful because of the highly repetitive sequence content on the distal ends of each contig. To determine the physical distance between and the orientation of the three contigs, we used high resolution pachytene fluorescence in situ hybridization (FISH) mapping. The RPD140 contig was positioned in the centromeric region of chromosome 12 between two large pericentric heterochromatin blocks, about 50 Mb from the TG618 contig on the short arm and 10 Mb from the CD22 contig on the long arm, respectively. Based on high resolution genetic and physical mapping, we conclude that the jointless-2 gene is located within or near the chromosome 12 centromere where 1 cM is approximately 25 Mb in length.
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References
Bernatzky R, Tanksley SD (1986) Methods for detection of single and low copy sequences in tomato on southern blots. Plant Mol Biol Rep 4:37–41
Budiman MA, Mao L, Wood TC, Wing RA (2000) A deep-coverage tomato BAC library and prospects toward development of an STC framework for genome sequencing. Genome Res 10:129–136
Chen M, Presting G, Barbazuk WB, Goicoechea JL, Blackmon B, Fang G, Kim H, Frisch D, Yu Y, Wing RA (2002) An integrated physical and genetic map of the rice genome. Plant Cell 14:537–545
Churchill GA, Giovannoni JJ, Tanksley SD (1993) Pooled-sampling makes high-resolution mapping practical with DNA markers. Proc Natl Acad Sci USA 90:16–20
Copenhaver GP, Nickel K, Kuromori T, Benito MI, Kaul S, Lin X, Bevan M, Murphy G, Harris B, Parnell LD (1999) Genetic definition and sequence analysis of Arabidopsis centromeres. Science 286:2468–2474
Entani T, Iwano M, Shiba H, Takayama S (1999) Centromeric localization of an S-RNase gene in Petunia hybrida Vilm. Theor Appl Genet 99:391–397
Feng Q, Zhang Y, Hao P, Wang S, Fu G, Huang Y, Li Y, Zhu J, Liu Y, Hu X (2002) Sequence and analysis of rice chromosome 4. Nature 420:316–320
Frary A, Presting GG, Tanksley SD (1996) Molecular mapping of the centromeres of tomato chromosomes 7 and 9. Mol Gen Genet 250:295–304
Ganal M, Young ND, Tanksley SD (1989) Pulsed-field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400
Hanson RE, Zwick M, Choi S, Islam-Faridi MN, McKnight TD, Wing RA, Price HJ, Stelly DM (1995) Fluorescence in situ hybridization of a bacterial artificial chromosome. Genome 38:646–657
Lapitan NLV, Broun SE, Kennard W, Stephen JL, Knudson DL (1997) FISH physical mapping with barley BAC clone. Plant J 11:149–156
Mao L, Begum D, Chuang HW, Budiman MA, Szymkowiak EJ, Irish EE, Wing RA (2000) JOINTLESS is a MADS-box gene controlling tomato flower abscission zone development. Nature 406:910–913
Mao L, Begum D, Goff SA, Wing RA (2001) Sequence and analysis of the tomato JOINTLESS locus. Plant Physiol 126:1331–1340
Marra MA, Kucaba TA, Dietrich NL, Green ED, Brownstein B, Wilson RK, McDonald KM, Hillier LW, McPherson JD, Waterston RH (1997) High throughput fingerprint analysis of large-insert clones. Genome Res 7:1072–1084
Peterson DG, Price HJ, Johnston JS, Stack SM (1996) DNA content of heterochromatin and euchromatin in tomato (Lycopersicon esculentum) pachytene chromosomes. Genome 39:77–82
Peterson DG, Pearson WR, Stack SM (1998) Characterization of the tomato (Lycopersicon esculentum) genome using in vitro and in situ DNA reassociation. Genome 41:346–356
Peterson DG, Lapitan NLV, Stack SM (1999) Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152:427–439
Ramanna MS, Prakken R (1967) Structure of and homology between pachytene and somatic metaphase chromosomes of tomato. Genetica 38:115–113
Rick CM (1956) Genetics and systematic studies on accessions of Lycopersicon from the Galapagos Islands. Ann J Bot 43:687–696
Rick CM (1980) Tomato linkage survey. Rep Tomato Genet Coop 30:2–17
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Sherman JD, Stack SM (1995) Two-dimensional spreads of synaptonemal complexes from solanaceous plants. High-resolution recombination nodule map for tomato (Lycopersicon esculentum). Genetics 141:683–708
Sherman JD, Herickhoff LA, Stack SM (1992) Silver staining two types of meiotic nodules. Genome 35:907–915
Soderlund C, Humphray S, Dunham A, French L (2000) Contigs built with fingerprints, markers, and FPC V4.7. Genome Res 10: 1772–1787
Tanksley SD, Ganal MW, Prince JP, de-Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Wing RA, Zhang H-B, Tanksley SD (1994) Map-based cloning in crop plants. Tomato as a model system. Genetic and physical mapping of jointless. Mol Gen Genet 242:681–688
Zhang H-B, Budiman MA, Wing RA (2000) Genetic mapping of jointless-2 to tomato chromosome 12 using RAPD and RFLP analysis. Theor Appl Genet 100:1183–1189
Zhong XB, Hans de Jong J, Zabel P (1996) Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH). Chromosome Res 4:24–28
Zhong X-B, Fransz PF, Wennekes-Eden J, Ramanna MS, van Kammen A, Zabel P, de Jong JH (1998) FISH studies reveal the molecular and chromosomal organization of individual telomere domains in tomato. Plant J 13:507–517
Zhong X-B, Bodeau J, Fransz PF, Williamson VM, van Kammen A, de Jong JH, Zabel P (1999) FISH to meiotic pachytene chromosomes of tomato locates the root-knot nematode resistance gene Mi-1 and the acid phosphatase gene Aps-1 near the junction of euchromatin and pericentromeric heterochromatin of chromosome arms 6S and 6L, respectively. Theor Appl Genet 98:365–370
Zwick MS, Hanson RE, McKnight TD, Islam-Faridi MN, Stelly DM, Wing RA, Price HJ (1997) A rapid procedure for the isolation of Cot-1 DNA from plants. Genome 40:138–142
Acknowledgements
This work was supported by grants from the USDA-CSREES and the NSF to RAW, Texas A&M University, the Clemson University Coker Endowed Chair, Korea Science and Engineering Foundation (KOSEF) and the University of Arizona.
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M.A. Budiman, S-B. Chang and S. Lee contributed equally to the work.
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Budiman, M.A., Chang, SB., Lee, S. et al. Localization of jointless-2 gene in the centromeric region of tomato chromosome 12 based on high resolution genetic and physical mapping. Theor Appl Genet 108, 190–196 (2004). https://doi.org/10.1007/s00122-003-1429-3
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DOI: https://doi.org/10.1007/s00122-003-1429-3