Skip to main content
Log in

AFLP analysis of genetic relationships among aromatic grapevines (Vitis vinifera)

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Genotypic diversity has been detected among aromatic grapevines (Vitis vinifera) by molecular markers (AFLPs). The 22 primer-pairs generated a total of 1,331 bands of which 564 (40%) were polymorphic over all the genotypes. The bootstrap analysis pointed out that a large number of polymorphic bands (200–400) has to be used for a better estimation of the genetic distances among genotypes; 383 polymorphic AFLP bands were used for the cluster and the principal coordinate analyses because they did not present missing data across all the genotypes. The cluster analysis (UPGMA), based on polymorphic AFLP markers, revealed no relationship between the Moscato and Malvasia grapevines. The Malvasias, unlike the Moscatos distinguished by their distinct muscat aroma, have to be considered a more complex group because it includes muscat and non-muscat grapevines. The principal coordinate analysis (PCO) confirmed the pattern of the cluster analysis only for those varieties which presented a low coefficient of dissimilarity, while for the other varieties there was no correspondence between the two analyses. The pattern of aggregation among aromatic grapevines in the cluster and principal coordinate analyses does not support any classification that might include an aromatic grapevine group in V. vinifera. Even though some synonyms and homonyms are present among aromatic grapevines (V. vinifera), genetic diversity exists among genotypes in AFLP markers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

References

  • Bourquin JC, Sonko A, Otten L, Walter B (1993) Restriction fragment length polymorphism and molecular taxonomy in Vitis vinifera L. Theor Appl Genet 87:431–438

    CAS  Google Scholar 

  • Bowers JE, Bandman EB, Meredith CP (1993) DNA fingerprint characterization of some wine grape cultivars. Am J Enol Vitic 44:266–274

    CAS  Google Scholar 

  • Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera). Genome 39:628–633

    CAS  Google Scholar 

  • Buscher N, Zyprian E, Bachmann O, Blaich R (1994) On the origin of the grapevine variety Muller-Thurgau as investigated by the inheritance of random amplified polymorphic DNA (RAPD). Vitis 33:15–17

    Google Scholar 

  • Cervera MT, Cabezas JA, Sancha JC, Martinez-de-Toda F, Martinez-Zapater JM (1998) Application of AFLPs to the characterization of grapevine Vitis vinifera L. genetic resources: a case study with accessions from Rioja (Spain). Theor Appl Genet 97:51–59

    CAS  Google Scholar 

  • Cettolini S (1893) Elenco delle principali uve sarde. Annuario R. Scuola di viticoltura e Enologia, Cagliari, Vol. III

  • Dalmasso G, Dell'Olio G, Malfatto P, Cosmo I, Polsinelli M, Sardi F, Prosperi V, Pirovano A, Bruni B, Breviglieri N, Casini E, Del Gaudio S, Giusto D, Panzera C, Mazzei A, Zappalá A (1964) Malvasie. In: Principali vitigni ad uva da vino coltivati in Italia. M.A.F., Roma

  • Dangl GS, Mendum ML, Prins BP, Walker MA, Meridith CP, Simon CJ (2001) Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genome 44:432–438

    Article  CAS  PubMed  Google Scholar 

  • Efron B, Tibshirani R (1991) Statistical analysis with computer age. Science 253:390–395

    Google Scholar 

  • Fanizza G, Corona MG, Resta P (2000) Analysis of genetic relationships among Muscat grapevines in Apulia (South Italy) by RAPD markers. Vitis 39:159–161

    CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Google Scholar 

  • Gogorcena Y, Arulsekar S, Dandekar AM, Parfitt DE (1993) Molecular markers for grape characterization. Vitis 32:183–185

    CAS  Google Scholar 

  • Lodhi MA, Weeden NF, Reich BI (1997) Characterization of RAPD markers in Vitis. Vitis 36:133–140

    Google Scholar 

  • Molon G (1906) Ampelografia. Hoepli U, Milano, Vol II

  • Nicosia G, Bambara G (1959) Il Malvasia di Lipari. In: Luigi Scialpi (ed) Estr da 'Vini d'Italia', Roma

  • Rohlf FJ (1997) NTSYS-pc-numerical taxonomy and multivariate analysis system. Exeter Software, Setauket, New York

  • Roy-Chevrier J (1900) Ampélographie rétrospective. Coulet et Fils, Montpellier

  • Scott KD, Ablett EM, Lee LS, Henry RJ (2001) AFLP markers distinguishing an early mutant of Flame Seedless grape. Euphytica 113:245–249

    Google Scholar 

  • Sefc KM, Lopez MS, Lefort F, Botta R, Roubelakis-Agelalakis KA, Ibanez I, Pejie HW, Wagner J, Glossl H, Stein-Kellner H (2000) Microsatellite variability in grapevine cultivars from different European region and evaluation of assignment testing to assess the geographic origin of the cultivar. Theor Appl Genet 100:489–505

    Article  Google Scholar 

  • Sensi E, Vignani R, Rohde W, Biricolti S (1996) Characterization of genetic biodiversity with Vitis vinifera L. Sangiovese and Colorino genotypes by AFLP and ISTR DNA marker technology. Vitis 35:183–188

    Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy. WH Freeman and Company, San Francisco

  • Stavrakakis MN, Biniari K (1998) Genetic study of grape cultivars belonging to the muscat family by random amplified polymorphic DNA markers. Vitis 37:119–122

    Google Scholar 

  • Thomas MS, Cain P, Scott NS (1994) DNA typing of grapevines: a universal methodology and database for describing cultivars and evaluating genetic relatedness. Plant Mol Biol 25:937–949

    Google Scholar 

  • Tivang JG, Nienhuis J, Smith OS (1994) Estimation of sampling variance of molecular marker data sites using the bootstrap procedure. Theor Appl Genet 89:259–264

    Google Scholar 

  • Viala P, Vermorel V (1909) Ampélographie. Masson et C, Paris, Tome VII

  • Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    CAS  PubMed  Google Scholar 

  • Xiamping Q, Lu J, Lamikanra O (1996) Genetic diversity in Muscadine and American bunch grapes based on random amplified polymorphic DNA (RAPD) analysis. J Am Soc Hort Sci 121:1020–1023

    Google Scholar 

  • Yap IV, Nelson RJ (1996) WINBOOT. A program for performing bootstrap analysis of binary data to determine the confidence limits of the UPGMA-based dendrogram. IRRI Discussion Paper Ser No. 14, International Rice Research Institute, Los Banos, The Philippines

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to G. Fanizza.

Additional information

Communicated by H.F. Linskens

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fanizza, G., Chaabane, R., Lamaj, F. et al. AFLP analysis of genetic relationships among aromatic grapevines (Vitis vinifera). Theor Appl Genet 107, 1043–1047 (2003). https://doi.org/10.1007/s00122-003-1354-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1354-5

Keywords

Navigation