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Impact of the Coffea canephora gene introgression on beverage quality of C. arabica

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Abstract

Lines of Coffea arabica derived from the Timor Hybrid (hybrid between C. arabica and C. canephora) are resistant to coffee leaf rust (Hemileia vastatrix) and to the nematode Meloidogyne exigua. The introgression of C. canephora resistance genes is suspected of causing a drop in beverage quality. Coffee samples from pure lines, compared in a Trial 1, and from F1 hybrids and parental lines from a half-diallel trial in a Trial 2, were studied for beverage quality, chemical composition and amount of introgressed genetic material. Chemical analyses (caffeine, chlorogenic acids, fat, trigonelline, sucrose) were carried out with near-infrared spectrometry by reflectance of green coffee. The number of amplified fragment length polymorphic (AFLP) markers introgressed from the Timor Hybrid varied from 1 to 37 for the lines studied. There were significant differences between lines for all of the biochemical compounds analysed and for the acidity and the overall standard of the beverage. Two lines (T17927, T17924) were significantly poorer than the controls for sucrose and beverage acidity. T17924 also had more chlorogenic acids and was poorer for the overall standard. However, two highly introgressed lines, T17934 and T17931 (25 and 30 AFLP markers, respectively), did not differ from the non-introgressed controls. There were no correlations between the number of AFLP markers and the chemical contents or beverage attributes. Significant correlations were found between the performance of the parents and their general combining ability for beverage quality. It was concluded that it should be possible to find lines with both the desired resistance genes and good beverage quality. Selection can avoid accompanying the introgression of resistance genes with a drop in beverage quality.

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References

  • Agrios GN (1997) How plants defend themselves against pathogens. In: Plant Pathology, 4th edn. Academic Press, San Diego, pp 93–114

  • Baradat P, Desprez-Loustau M (1997) Analyse diallèle et intégration de la sensibilité à la rouille courbeuse dans le programme d'amélioration du pin maritime. Ann Sci For 54:83–106

    Google Scholar 

  • Baradat P, Labbé T (1995) opep, un logiciel intégré pour l'amélioration des plantes pérennes. In: CIRAD (eds) Traitements statistiques des essais de sélection. Stratégies de sélection des plantes pérennes. Montpellier France, pp 303–330

  • Bertrand B, Aguilar G, Bompard E, Rafinon A, Anthony F (1997) Comportement agronomique et résistance aux principaux déprédateurs des lignées de Sarchimors et Catimors au Costa Rica. Plantation Rech Dev 5:312–321

    Google Scholar 

  • Bertrand B, Aguilar G, Santacreo R, Anzueto F (1999) El mejoramiento genético en América Central. In: Bertrand B, Rapidel B (eds) Desafios de la caficultura centroamericana. IICA, San José, Costa Rica, pp 407–456

  • Bertrand B, Anthony F, Lashermes P (2001) Breeding for resistance to Meloidogyne exigua in Coffea arabica by introgression of resistance genes of Coffea canephora. Plant Pathol 50:1–8

    Article  Google Scholar 

  • Bettencourt A (1973) Consideraçoes gerais sobre o 'Hibrido do Timor'. Instituto Agronomico de Campinas, Circular no. 31, Campinas, Brazil

  • Carvalho A, Eskes AB, Castillo J, Sreenivasan MS, Echeverri JH, Fernandez CE, Fazuoli C (1989) Breeding programs. In: Kushalappa AC, Eskes AB (eds) Coffee rust: epidemiology, resistance and management. CRC Press, Boca Raton, pp 293–331

    Google Scholar 

  • Castillo Z (1990) Mejoramiento genetico del café en Colombia. In: Centro Nacional de Investigaciones de Café CENICAFE (eds) 50 años de Cenicafé, 1938–1988. Conferencias conmemorativas, Chinchina, Colombia, pp 46–53

  • Charrier A, Berthaud J (1985) Botanical classification of coffee. In: Clifford MN, Wilson KC (eds) Coffee: botany, biochemistry and productions of beans and beverage. Croom Helm, London, pp 13–47

    Google Scholar 

  • Clifford MN (1985) Chemical and physical aspects of green coffee and coffee products. In: Clifford MN, Wilson KC (eds) Coffee: botany, biochemistry and productions of beans and beverage. Croom Helm, London, pp 305–374

    Google Scholar 

  • Downey G, Boussion J (1996) Authentification of coffee bean variety by near-infrared reflectance spectroscopy of dried extract. J Sci Agric 71:41–49

    Article  CAS  Google Scholar 

  • Downey G, Robert P, Bertrand D, Kelly PM (1990) Classification of commercial skim milk powders according to heat treatment using factorial discriminant analysis of NIR spectra. Appl Spectrosc 44:150–154

    CAS  Google Scholar 

  • Downey G, Boussion J, Beauchêne D (1994) Authentification of whole and ground coffee beans by near infrared reflectance spectroscopy. J Near-Infrared Reflectance Spectroscopy 2:85–92

    CAS  Google Scholar 

  • Fazuoli LC, Carvalho A, Monaco LC, Texeira AA (1977) Qualidade da bebida do café ICATU. Bragantia 36:165–172

    Google Scholar 

  • Gonçalvez W, Pereira A (1998) Resistência do cafeeiro a nematóides IV-reaçao de cafeeiros derivados do Híbrido de Timor a Meloidogyne exigua. Nematol Bras 22:39–50

    Google Scholar 

  • Grandillo S, Bernacchi D, Fulton TM, Zamir D, Tanksley SD (1999) Advanced backcross QTL analysis: a method for the systematic use of exotic germplasm in the improvement of crop quality. In: Scarascia Mugnozza GT, Porceddu E, Pagnotta MA (eds) Genetics and breeding for crop quality and resistance. Kluwer, Dordrecht, pp 291–299

  • Guerrero G, Suárez M, Moreno G (2001) Chlorogenic acids as a potential criterion in coffee genotype selections. J Agric Food Chem 49:2454–2458

    Article  CAS  PubMed  Google Scholar 

  • Guyot B, Pentga E, Vincent JC (1988) Analyse qualitative d'un café Coffea canephora var. robusta en fonction de la maturité. Café Cacao Thé 32:127–140

  • Guyot B, Davrieux F, Manez JC, Vincent JC (1993) Détermination de la caféine et de la matière sèche par spectrométrie proche infrarouge. Applications aux café verts Robusta et aux cafés torréfiés. Café Cacao Thé 37:53–64

  • Herrington ME, Brown PJ, Carr AR (1983) Introgression as a source of bitterness in watermelon. HortScience 21:1237–1238

    Google Scholar 

  • Keuls M, Garretsen F (1977) A general method for the analysis of genetic variation in complete and incomplete diallels and North Carolina II designs. Part 1: procedures and general formulas for the random model. Euphytica 26:537–551

    Google Scholar 

  • Kushalappa AC, Eskes AB (1989) Coffee rust: epidemiology, resistance and management. CRC Press, Boca Raton

    Google Scholar 

  • Ky CL, Louarn J, Guyot B, Charrier A, Hamon S, Noirot M (1999) Relations between and inheritance of chlorogenic acid contents in an interspecific cross between Coffea pseudozanguebariae and Coffea liberica var. 'dewevrei'. Theor Appl Genet 98:628–637

    Article  CAS  Google Scholar 

  • Lashermes P, Cros J, Marmey P, Charrier A (1996) Use of random amplified DNA markers to analyse genetic variability and relationships of Coffea species. Genet Resources Crop Evol 40:91–99

    Google Scholar 

  • Lashermes P, Combes MC, Robert J, Trouslot P, D'Hont A, Anthony F, Charrier A (1999) Molecular characterisation and origin of Coffea arabica L. genome. Mol Gen Genet 261:259–266

    CAS  PubMed  Google Scholar 

  • Lashermes P, Andrzejewski S, Bertrand B, Combes MC, Dussert S, Graziosi G, Trouslot P, Anthony F (2000a) Molecular analysis of introgressive breeding in coffee (Coffea arabica L.). Theor Appl Genet 100:139–146

    CAS  Google Scholar 

  • Lashermes P, Combes MC, Topart P, Graziosi G, Bertrand B, Anthony F (2000b) Molecular breeding in coffee (Coffea arabica L.). In: Sera T, Soccol CR, Pandey A, Roussos S (eds) Coffee biotechnology and quality. Kluwer, Dordrecht, pp 134–146

  • Moreno G, Moreno E, Cadena G (1995) Bean characteristics and cup quality of the Colombia variety (Coffea arabica) as judged by international tasting panels. In: 16th Int. Sci Colloq Coffee. ASIC, Kyoto, pp 574–583

  • Owuor JB (1988) An assessment of the cup quality of the new disease resistant Coffea arabica cultivar RUIRU 11 in Kenya. Kenya Coffee 53:333–336

    Google Scholar 

  • Perez DP, Sanchez MT, Cano G, Garrido A (2001) Authentication of green asparagus varieties by near-infrared reflectance spectroscopy. J Food Sci 66:323–327

    CAS  Google Scholar 

  • Puerta GI (1998) Calidad en taza de las variedades de Coffea arabica L. cultivadas en Colombia. Cenicafé 49:265–278

    Google Scholar 

  • Puerta GI (2000) Calidad en taza de algunas mezclas de variedades de café de la especie Coffea arabica L. Cenicafé 51:5–19

    Google Scholar 

  • Scanlon MG, Pritchard M, Lorne RA (1999) Quality evaluation of processing potatoes by near infrared reflectance. J Sci Food Agric 79:763–771

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    CAS  PubMed  Google Scholar 

  • Williams P, Norris K (1990) Near infrared technology in the agricultural and food industries. American Association of Cereal Chemists, St Paul, Minn.

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Acknowledgements

We thank the members of the 'jury' for the organoleptic analysis at ICAFE, particularly Juan-Carlos Selva and Jose María Alpizar (ICAFE). This work was supported by the European Community through the International Scientific Co-operation Programme (INCO-DC Contract ERBIC18CT970181).

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Author notes

  1. B. Guyot

    Present address: CIRAD, BP 5035, Laboratoire d'analyses sensorielles, 34032 Montpellier, France,

  2. F. Anthony & P. Lashermes

    Present address: IRD (ex. ORSTOM), Genetrop, B.P. 5045, 34032, Montpellier Cedex, France,

Authors and Affiliations

  1. IICA/PROMECAFE, Ap. Postal 55, 2200, Coronado, Costa Rica

    B. Bertrand, B. Guyot, F. Anthony & P. Lashermes

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  1. B. Bertrand
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Correspondence to B. Bertrand.

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Communicated by H. Nybom

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Bertrand, B., Guyot, B., Anthony, F. et al. Impact of the Coffea canephora gene introgression on beverage quality of C. arabica . Theor Appl Genet 107, 387–394 (2003). https://doi.org/10.1007/s00122-003-1203-6

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  • DOI: https://doi.org/10.1007/s00122-003-1203-6

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