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Embryonale und adulte Stammzellen für Tissue Engineering in der Urologie

Embryonic and adult stem cells for tissue engineering in urology

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Zusammenfassung

Der Einsatz von Stammzellen eröffnet neue Therapiemöglichkeiten. Bisher unheilbare Erkrankungen wie Morbus Parkinson oder Diabetes mellitus sollen durch deren Einsatz heilbar werden. Obwohl eine Stammzelle genau definiert ist, wird der Begriff wahllos verwendet. Undifferenzierte Zellen (Stammzellen oder Progenitorzellen) werden aus pränatalen (embryonalen) und postnatalen Geweben isoliert. Im Folgenden sollen ein Überblick über embryonale und adulte Stammzellen gegeben und der mögliche Einsatz auf dem Gebiet des Tissue Engineerings in der Urologie aufgezeigt werden.

Abstract

Stem cells are considered a possible alternative treatment for degenerative diseases. Parkinson’s disease and diabetes mellitus may become curable using stem cells. The term “stem cell” is used very broadly, although it has been well defined. Undifferentiated cells (stem cells and progenitor cells) are isolated from prenatal and postnatal tissues. This review provides an overview of embryonic and adult stem cells and describes potential applications in the field of tissue engineering in urology.

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Literatur

  1. Al-Awqati Q, Oliver JA (2002) Stem cells in the kidney. Kidney Int 61: 387–395

    Article  PubMed  Google Scholar 

  2. Alison MR, Poulsom R, Forbes S, Wright NA (2002) An introduction to stem cells. J Pathol 197: 419–423

    Article  PubMed  Google Scholar 

  3. Andrews PW, Oosterhuis J, Damjanov I (1987) Teratocarcinomas and embryonic stem cells: a practical approach. IRL Press, Oxford

    Google Scholar 

  4. Bartsch G Jr, Yoo JJ, De Coppi P et al. (2002) Dermal stem cells for pelvic and bladder reconstruction. J Urol [Suppl 4] 163: 181

  5. Cannon TW, Lee JY, Somogyi G et al. (2003) Improved sphincter contractility after allogenic muscle-derived progenitor cell injection into the denervated rat urethra. Urology 62: 958–963

    Article  PubMed  Google Scholar 

  6. De Bari C, Dell’Accio F, Tylzanowski P, Luyte FP (2001) Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum 44: 1928–1942

    Article  PubMed  Google Scholar 

  7. El-Nahas AM (2003) Plasticity of kidney cells: role in kidney remodeling and scarring. Kidney Int 64: 1553–1563

    Article  CAS  PubMed  Google Scholar 

  8. First NL, Thompson J (1998) From cows stem therapies? Nat Biotechnol 16: 620–621

    Article  CAS  PubMed  Google Scholar 

  9. Frimberger D, Lakshmanan Y, Morales N, Gearhart JD, Gearhart JP (in press) Human pluripotent stem cells in bladder regeneration using a rodent model. J Urol (in press)

  10. Goldring K, Partridge T, Watt D (2002) Muscle stem cells. J Pathol 197: 457–467

    Article  PubMed  Google Scholar 

  11. Hadjantonakis AK, Nagy A (2000) FACS for the isolation of individual cells from transgenic mice harboring a fluorescent protein marker. Genetics 27: 95–98

    Google Scholar 

  12. Heim DA, Hanazono Y, Giri N et al. (2000) Introduction of a xenogenic gene via hematopoetic stem cells leads to specific tolerance in a rhesus monkey model. Mol Ther 1: 533–544

    Article  CAS  PubMed  Google Scholar 

  13. Horner PJ, Gage FH (2000) Regenerating the damaged central nervous system. Nature 407: 963–970

    Article  CAS  PubMed  Google Scholar 

  14. Huss R (2000) Isolation of primary and immortalized CD34-hematopoietic and mesenchymal stem cells from various sources. Stem Cells 18: 1–9

    Article  CAS  PubMed  Google Scholar 

  15. Itskovitz-Eldor J, Schulding M, Karsenti D, Yanuka O (2000) Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. Mol Med 6: 88–95

    CAS  PubMed  Google Scholar 

  16. Jiang Y, Jahagirdar BN, Reinhardt RL et al. (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418: 41–49

    Article  CAS  Google Scholar 

  17. Jiang Y, Henderson D, Blackstad M, Chen A, Miller RF, Verfaillie CM (2003) Neuroectodermal differentiation from mouse multipotent adult progenitor cells. Proc Natl Acad Sci USA [Suppl 1] 100: 11.854–11.860

    Google Scholar 

  18. Kerr DA, Llado J, Shamblott MJ et al. (2003) Human embryonic germ cell derivates facilitate motor recovery of rats with diffuse motor neuron injury. J Neurosci 23: 5131–5140

    CAS  PubMed  Google Scholar 

  19. Kim JH, Auerbach JM, Rodriguez-Gomez JA et al. (2001) Dopamin neurons, derived from embryonic stem cells function in an animal model of Parkinson disease. Nature 418: 50–56

    Article  Google Scholar 

  20. Klug MG, Soonpaa MH, Koh GY et al. (1996) Genetically selected cardiomyocytes from differentiating embryonic stem cells from stable intracardiac grafts. J Clin Invest 98: 216–224

    CAS  PubMed  Google Scholar 

  21. Kropp BP, Rippy MK, Badylak SF et al. (1996) Regenerative urinary bladder augmentation using small intestinal submucosa: urodynamic and histopathologic assessment in long-term canine bladder augmentations. J Urol 155: 2098–2104

    Article  CAS  PubMed  Google Scholar 

  22. Lee JY, Qu-Petersen Z, Cao B et al. (2000) Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing. J Cell Biol 150: 1085–1100

    Article  CAS  PubMed  Google Scholar 

  23. Mitsui T, Kakizaki H, Tanaka H, Shibata T, Matsuoka I, Koyanagi T (2003) Immortalized neural stem cells transplanted into the injured spinal cord promote recovery of voiding function in the rat. J Urol 170: 1421–1425

    Article  PubMed  Google Scholar 

  24. Oberpenning F, Meng J, Yoo JJ, Atala A (1999) De novo reconstitution of a functional mammalian urinary bladder by tissue engineering. Nat Biotechnol 17: 149–155

    Article  CAS  PubMed  Google Scholar 

  25. Okano H (2002) Neural stem cells: progression of basic research and perspective for clinical application. Keio J Med 51: 115–128

    CAS  PubMed  Google Scholar 

  26. Orlic D, Kajstura J, Chimenti S et al. (2001) Bone marrow cells regenerate infarcted myocardium. Nature 410: 701–705

    Article  Google Scholar 

  27. Pittenger MF, Mackay AM, Beck SC et al. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143–147

    Article  CAS  PubMed  Google Scholar 

  28. Raff M (2003) Adult stem cell plasticity: fact or artifact? Annu Rev Cell Dev Biol 19: 1–22

    Article  CAS  PubMed  Google Scholar 

  29. Rohwedel J, Maltsev V, Bober E, Arnold HH, Hescheler J, Wobus AM (1994) Muscle cell differentiation of embryonic stem cells reflects myogenesis in vivo: developmentally regulated expression of myogenic termination genes and functional expression of ionic currents. Dev Biol 164: 87–101

    Article  CAS  PubMed  Google Scholar 

  30. Schmidt K, Glaser G, Wernig A, Wegner M, Rosorius O (2003) Sox8 is a specific marker for muscle satellite cells and inhibits myogenesis. J Biol Chem 278: 29.769–29.775

    Google Scholar 

  31. Shamblott M, Axelman J, Littelfield JW et al. (2001) Human embryonic germ cell derivates express a broad range of developmentally distinct markers and proliferate extensively in vitro. Proc Natl Acad Sci USA 98: 113–118

    Article  CAS  PubMed  Google Scholar 

  32. Soria B, Roche E, Berna G et al. (1978) Insulin secreting cells derived from embryonic stem cells normalize glycaemia in fed a coline deficient diet. Cancer Res 38: 1092–1098

    PubMed  Google Scholar 

  33. Stevens LC (1979) The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol 21: 364–382

    Google Scholar 

  34. Strasser H, Marksteiner R, Margreiter E, Frauscher F, Bartsch G, Hering S (2004) Stem cell therapy in treatment of urinary incontinence: first clinical results. J Urol 171: 130

    PubMed  Google Scholar 

  35. Thompson JA, Itskovitz-Eldor J, Shapiro SS et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145–1147

    Article  CAS  PubMed  Google Scholar 

  36. Tintut Y, Alfonso Z, Saini T et al. (2003) Multilineage potential of cells from the artery wall. Circulation 108: 2505–2510

    Article  PubMed  Google Scholar 

  37. Toma JG, Akhavan M, Fernandes KJ et al. (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3: 778–784

    Article  CAS  PubMed  Google Scholar 

  38. Verfaillie CM, Pera MF, Lansdorp PM (2002) Stem cells: hype and reality. Hematology 2002: 369–391

    Article  Google Scholar 

  39. Westphal CH, Lederer P (1997) Transposon-generated knock-out and knock-in in gene targeting constructs for use in mice. Curr Biol 7: 155–157

    Article  Google Scholar 

  40. Xu C, Inokuma MS, Denham J et al. (2001) Feeder free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19: 971–974

    Article  CAS  PubMed  Google Scholar 

  41. Yiou R, Lefaucheur JP, Atala A (2003) The regeneration process of the striated urethral sphincter involves activation of intrinsic satellite cells. Anat Embryol (Berl) 206: 429–435

    PubMed  Google Scholar 

  42. Yokoyama T, Huard J, Pruchnic R et al. (2001) Muscle-derived cell transplantation and differentiation into lower urinary tract smooth muscle. Urology 57: 826–831

    Article  CAS  PubMed  Google Scholar 

  43. Yoo JJ, Ashkar S, Atala A (1996) Creation of functional kidney structures with excretion of urine-like fluid in vivo. Pediatrics 98S: 605

    Google Scholar 

  44. Zammit P, Beauchamp J (2001) The skeletal muscle satellite cell: stem cell or son of stem cell? Differentiation 68: 193–204

    Article  CAS  PubMed  Google Scholar 

  45. Zhang Y, Kropp BP, Moore P et al. (2000) Coculture of bladder urothelial and smooth muscle cells on small intestinal submucosa: potential applications for tissue engineering technology. J Urol 164: 928–935

    Article  CAS  PubMed  Google Scholar 

  46. Zhang SC, Wernig M, Duncan ID et al. (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 19: 1129–1133

    Article  CAS  PubMed  Google Scholar 

  47. Zuk PA, Zhu M, Mizuno H et al. (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7: 211–228

    Article  CAS  PubMed  Google Scholar 

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Bartsch, G., Frimberger, D. Embryonale und adulte Stammzellen für Tissue Engineering in der Urologie. Urologe [A] 43, 1229–1236 (2004). https://doi.org/10.1007/s00120-004-0691-6

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  • DOI: https://doi.org/10.1007/s00120-004-0691-6

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