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Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector

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Abstract

 Constitutive, high-level expression of the potentially therapeutic WAF-1/CIP-1/SDI-1 gene is incompatible with cell growth. A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells. Reduced cell growth due to a greater proportion of cells being in the G0/G1 phase of the cell cycle was observed when WAF-1/CIP-1/SDI-1 expression was activated by addition of glucocorticoid hormone. This system demonstrates the potential long-term therapeutic use of WAF-1/CIP-1/SDI-1 delivered by retroviral vectors for inhibiting the growth of rapidly proliferating cells. Moreover, the conditional expression of genes such as WAF-1/CIP-1/SDI-1 from such retroviral vectors may facilitate analysis of their function.

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Authors and Affiliations

  1. Institute of Molecular Virology GSF-National Research Center for Environment and Health, Oberschleißheim, and Medizinische Klinik I, Klinikum Großhadern, Universität München, Munich, Germany, , , , , , DE

    Stefan Mrochen

  2. Institute of Molecular Virology GSF-National Research Center for Environment and Health, Oberschleißheim, and Bavarian Nordic Research Institute, Munich, Germany, , , , , , DE

    Dieter Klein

  3. Medizinische Klinik I, Klinikum Großhadern, Universität München, Munich, Germany, , , , , , DE

    Sigrid Nikol

  4. Huffington Center on Aging, Division of Molecular Virology, and Departments of Cell Biology and Medicine, Baylor College of Medicine, Houston, USA, , , , , , US

    James R. Smith

  5. Bavarian Nordic Research Institute, Munich, Germany, , , , , , DE

    Brian Salmons

  6. Institute of Virology, University of Veterinary Sciences, Josef-Baumann-Gasse 1, A-1210 Vienna, Austria and Institute of Molecular Virology GSF-National Research Center for Environment and Health, Oberschleißheim, Germany , , , , , , DE

    W. H. Günzburg

Authors
  1. Stefan Mrochen
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  2. Dieter Klein
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  3. Sigrid Nikol
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  4. James R. Smith
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  5. Brian Salmons
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  6. W. H. Günzburg
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Received: 24 March 1997 / Accepted: 5 July 1997

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Mrochen, S., Klein, D., Nikol, S. et al. Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector. J Mol Med 75, 820–828 (1997). https://doi.org/10.1007/s001090050171

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  • DOI: https://doi.org/10.1007/s001090050171

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